FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_81.3410000000ab06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_81.3410000000ab06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAAGCAGCTTGGAGAAGAGCGAGAGAGAGTAGGAATATCCATTTGAA11.694915254237288No Hit
GTATCAACGCAGAGTACGAGAGAGAAAATGAGCCAATATGATTCACCAGAA11.694915254237288No Hit
GTTTGATTGCTGAAGATGGATGATTCATATCTGAAGGATCAAAAATGAAGC11.694915254237288No Hit
GAGCTGTACTGTTCCTGAGAAGAAAAAGCTTGAAGATGAGAATAGCATTGA11.694915254237288No Hit
TACCACTGGTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCT11.694915254237288No Hit
CCGGAAGAAGAGTCTGGTGTGAAGCATAAAAGGGTCTTAATTAATGATCTA11.694915254237288No Hit
AGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCCCGT11.694915254237288No Hit
ATTGTAGAACTCGGAGATTTTGGAGAAATGTATAGTTGCGATTCTTTGATT11.694915254237288No Hit
GTGTAAACTCCTCAGAGGAACTCCATTAATCCACTCTCAACCTGTCTCTTA11.694915254237288No Hit
TCCATGACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTC11.694915254237288No Hit
ATGCAAATAGCTCAAGAGGACGCTGATATGCCAAAAGTGTTGATGTCCTGT11.694915254237288No Hit
ATGATGAGATCGACGAAAGCGCTTAGTATGAACTTGTGCTGTCTCTTATAC11.694915254237288No Hit
CTCCATAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTTCCTAT11.694915254237288No Hit
CCATAAATTCAGAACCAAGAAACATCTCTGAGAGACTAAACGAAGATGTAT11.694915254237288No Hit
ATAGTAGACCTTCCCAGAGCAGAGGACTAGACGTCTGATACCTTCTTCAAG11.694915254237288No Hit
GTCTCAAACCAAGTTCGATCTAAAAGCCTTTTCCTTTAGATTCTCTCCAAC11.694915254237288No Hit
AGTCAACGGGTGAGTTAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGAA11.694915254237288No Hit
ATTGAAGGCATCTGGAAAGGAAGTATCTGCACAAAACAACGATGTTTTGAA11.694915254237288No Hit
GATGGAAATGAGGAACTTGTTGGAACTGGAGCAAAGGCTGTCTCTTATACA11.694915254237288No Hit
CTTAAATACATTCCTCATAGGTTAGCCGGAGTAACACTAGTTGCTCCAGTG11.694915254237288No Hit
AGATTGTTCACATCTGTATCCATCATATGCCACACCATTCTCCTGTCTCTT11.694915254237288No Hit
ATCAACGCAGAGTACGGGCAATCATGTTTCAACGTTTAGAAGCTGTCTCTT11.694915254237288No Hit
CTCCAAACCACTTGATGTTCGGAATACCAGATCCTCCTTGAAGCAGCATAT11.694915254237288No Hit
GGATGGGGGCCGGCGATGCGTCCTGGTCGGATGCGGAACGGAGCAATCCGG11.694915254237288No Hit
AATAAACCACCTCATCAACAAAGAGAATTCTGAAGGTGTTGTTCTGCTTGG11.694915254237288No Hit
TGGAAGTACCGTGCTTTGTGATACACGCTCCAAGAGTCGAGTAGTTTGGGA11.694915254237288No Hit
GGAGGATTTAGATTGTAGTTATCTTCTTCTGCTCTCATCATCATCGGTCCC11.694915254237288No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT11.694915254237288No Hit
ACACAAGTCGTCGGATCTCGTCTTTACGAATAGACAAAAAGCTATTGAGAC11.694915254237288No Hit
AAATATTTCGTAGCCCTAGTTCGATTCTTGAATTTTGCTAGATGCTGTCTC11.694915254237288No Hit
ATATGTGACTGATCGGAGCCCTTCTAGTACAGATAATACTCTGTCTCTTAT11.694915254237288No Hit
AGGTAACCATCAACATATTGGTCACGCTAGTACTGTTCCTGTCTCTTATAC11.694915254237288No Hit
CTTACAGACTCCACAAGTGATATTTCATGGCAAGAACTTGTCGAGGCTAGA11.694915254237288No Hit
TTTTTGTAGCTGCTGAAAGATTTGAGATTGAAGCTTTTGCTTGTCTCGGGA11.694915254237288No Hit
CTATTACGTCTTTCATCGCCTCTGACTGCCAAGGCATCCACCACATGCACT11.694915254237288No Hit
AACAACAACACAACATGTAATCATAAGAAAGAAAGCTTTTTGAAACAAAAA11.694915254237288No Hit
GGGCTCAGGAGTGAACAATTGGATCGTTCATATGGAAGGAGGAGGATGGTG11.694915254237288No Hit
GATCTCAAGGAAGTGGCGACTCTTGATGCTCTTCAGAGTAAGGATACTGTC11.694915254237288No Hit
ACTTAGATGTTTCAGTTCGCTAAGTTTTCAAAGTCCAAAGAGCGCAGACTA11.694915254237288No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC11.694915254237288No Hit
GTTTAGAGGGAGCTTTCCTTCGATTACAAGGTCAAGATAACTCCGGATCTG11.694915254237288No Hit
ATGTATGTATGCAATACAAAATTGTGAAAGAAGGTGAACGAACTAGTCATG11.694915254237288No Hit
ACCTTAAACATTACTCCTTTCCTTCAAATGTATCACACAATGTCAAAAACT11.694915254237288No Hit
GTGCTGCAGACCACGATCACGGCAGCGACGTCTCCACAAGCGTATCAAAAG11.694915254237288No Hit
TGACCAAGGTGAGAGCTGAGTAGAAATCTTATCAGCAGATACACCCTGCCT11.694915254237288No Hit
AGAGGAGACTCCAACTTTTGCATTAGTTTCAGCAGAATCATCGGTTTTGTG11.694915254237288No Hit
ATCTATGACATCTGCAGACGTTCCCTAAGCATTGAGACTGTCTCTTATACA11.694915254237288No Hit
CTCTTCCTGCAATCGGAAAGAGATACCTCGAACGGGTCCCTTCTGGATCTG11.694915254237288No Hit
GAAGTGGATGATGCAGACATTTTTGCTTCTTGGGGCGTGGATTATCTGAAA11.694915254237288No Hit
GATGTATCTTCAGTGCTATATCAACGAGAAGGGTGAGAAGGTTTACACAAC11.694915254237288No Hit
GTCAGACACTTACCAACCAAGTCAAACACATTGTACGCTGCAATAAGCCTG11.694915254237288No Hit
GATTGATTACCTTGAGCCACTTGTAGAGAAAGTTATAAAGCCAGATGCAGA11.694915254237288No Hit
GAATAGGTTTCATCCTGAAGTCTGGCGACAAGTTATGCCCTGTCTCTTATA11.694915254237288No Hit
GGTTTCGATCCAATCGTCATCGTTATCAGGAAGACTGGTTTTGGCGGTTAC11.694915254237288No Hit
CCATTGTTCTGTTGAATCCGTCGGTAGCCGGAGCAATCATGTACAAACAGT11.694915254237288No Hit
CTTTTCCTCTCTCAGCTTCAGAACCTGCTCTAATATCTCAATCGCATCCTC11.694915254237288No Hit
ATGTGGAAGCGACCACTTACCACCCACCGCTATTGAACTTAACGCTTCACA11.694915254237288No Hit
CATTATACCTTCCTCTTCTTTACATAACGTTCCTCGCAGACTTTTTCCAGG11.694915254237288No Hit
GAGTTTTAGAGTCTTGTCTTGGCTGACTGCATGTTTGTGAAGGAATTTACT11.694915254237288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG50.045.06
GAGAAAA50.045.022
TTCACCA50.045.042
CCAATAT50.045.033
AGCCAAT50.045.031
ATGATTC50.045.038
ACGCAGA50.045.07
GAAAATG50.045.024
TGATTCA50.045.039
GTATCAA50.045.01
TATGATT50.045.037
GAGCCAA50.045.030
TCAACGC50.045.04
CAGAGTA50.045.010
CAATATG50.045.034
AGTACGA50.045.013
AGAGTAC50.045.011
ACGAGAG50.045.016
GAGTACG50.045.012
AGAAAAT50.045.023
Produced by FastQC (version 0.11.2)