FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_79.3410000000aaeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_79.3410000000aaeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC24.081632653061225No Hit
CTGCTTTCTCTATCTCATGTCTGTTTTAAGGTTTCTTCGGTCACTTAAGCT12.0408163265306123No Hit
CTTGTATGGTGAGAAGGAATGGTACTTCTTCTCTCCCAGGGACAGAAAACT12.0408163265306123No Hit
GGGGAAGACATAGCAAGGCACGATTCACAACGGATTGTATGGCTTCCTCGT12.0408163265306123No Hit
TAGCTGGATCATCCTTGGAGTTAGAGGCAACGTATCCTCTCTTAAGATCCT12.0408163265306123No Hit
ATTCTACACTTTTAGTTAGACAAGACTAAGTCTTGCTGCATGGAAGTGTAA12.0408163265306123No Hit
CCTCTTCAAATTGCGGCTGAGCGTGAAATCGCGAGGTCGTTGATCTGTCTC12.0408163265306123No Hit
ACTCAATTCGCTGCTTTACCTCTCAATCTCTGCAAATCCTGTCTCTTATAC12.0408163265306123No Hit
TCGTGAAGGAGAGCAAGTTTTTGGTGTTGTTCACATCCTGTCTCTTATACA12.0408163265306123No Hit
AATATCAAAGTTAGAGGAGGCTCTTTACAACATCCAATTTGAACAACATTG12.0408163265306123No Hit
GTGCGTCATTTAGCAAACCAGGAGGACGATTGATAACGTGAACCAAAGGAG12.0408163265306123No Hit
GTCCATTGACAACACAAGACTCAATTCACCAATGACACACTAATTGCTGTC12.0408163265306123No Hit
GGTAAATACCTTTATTCCGATGAGGTCGCAGAATTTCTTCTGTGTAATTGC12.0408163265306123No Hit
CCTCAGAGACACCAACAAGTCTCTCCTTCATCTTGTGGTACTTCTTAGGAT12.0408163265306123No Hit
AAGTACAATACTCCTAAGTATCGTTTTGTTGTCCGGTTTACCAACAAAGAC12.0408163265306123No Hit
GTGCTACTTGATCGGAAGAAGGTTTAGCCACACACCAAGTCCTGTCTCTTA12.0408163265306123No Hit
TGCTTGTTTAGCTCCTGTGAGCTCATCACTTTCCTGCTCAGTATGTTGTAT12.0408163265306123No Hit
GTGTGTTTATAATCCCAGGCTCATGGAGTCTTGATCAGTGTGTCTGAGTTG12.0408163265306123No Hit
AAACAAAAGAGAGGGAACAACACTTTTGCCATTCCCTCTCTCTCCCTGTCT12.0408163265306123No Hit
GGTATAGCATTGGAAATGTAAATGAGCTAAATACCTGTCTCTTATACACAT12.0408163265306123No Hit
TACCACCACTTCTATCAACGGTATTGGTCTCTGCAACTTCAAAAGATGGAA12.0408163265306123No Hit
CCATAACCTTAACGGTCTTAAAGCTCATAGCAGTCAAAGCGAAATAGATAG12.0408163265306123No Hit
GTATCAACGCAGAGTACGGGGCAGAAATCACATTGCGTTAGCATCCGCAGG12.0408163265306123No Hit
CGAATACTACTAAGAAGCGTGAGATAGAAGACAACTCGAGAAAATTAGGTG12.0408163265306123No Hit
TTGGAAGATTATCCAATGGTGATTGTTCAGATTCCTATGTGCAATGAAAAG12.0408163265306123No Hit
TATCAACGCAGAGTACGGGAAATTCGAAGATGGATGGGAGAAGAGATGGGT12.0408163265306123No Hit
GTATTTAATACGGCCTAATTGTGTACAACCTAAGTTACCGCCTTCACCAAT12.0408163265306123No Hit
TGTTTGGAGTCTATATTTGCTCTTTGTGTCCCAATCCTGTCGTATTGAGAG12.0408163265306123No Hit
CTGCTGCTTCAATCAGTGAGATAGAAACTGCAATGTCAAGCTACTGTCTCT12.0408163265306123No Hit
ATCACATGCAATGCCACATGAAGAAGAAGCACAACATGATTCTCAGTCACA12.0408163265306123No Hit
AGCATATCCTGATGAGAAAAAGTCTGCAATTTCGACTGAATTTGAGAAACT12.0408163265306123No Hit
CTATTGACACGAGCCTTCACAGAGGTCAAGGGGTCAAGCGCTGTCTCTTAT12.0408163265306123No Hit
GAAGAGAGTGGAACAGTGATGTATCAGACGTTTGAGAAGCTTGGTAAGTAC12.0408163265306123No Hit
AGATATACCAGTTCGAGAAAAAACAAAACATAAAACTGATCACCGGTAACG12.0408163265306123No Hit
GATCAAATATGAAGCAGGCTTGACCCAACTGTCCCCTTTTGGAGTATAGAA12.0408163265306123No Hit
GTTTCGGGTTGACCACAAAACCATACAAAATGTAGACTCCCTTTTAGCTGT12.0408163265306123No Hit
GTTATTAGCAGTCAAGCGCTTCCTGGTCTGTTAAACCTTTTGAAGAACACA12.0408163265306123No Hit
GACTGGTGTGAGGTAGAATCTTAGGGTCTTCTGATTTGCATTTTCTTGATG12.0408163265306123No Hit
ATTCTCAGCTAACAAAGAGAGGAAGTTCTTAGTGCTGTCTCTTATACACAT12.0408163265306123No Hit
ATGCTTAACTCAATACTCAGCAAAAAAAGAAGAAGAAGCAGAAAAGGCGCA12.0408163265306123No Hit
AGCTTGAGATCTCTATGTTTTGTGTTGAACGTTTAGCATTATCTCTATTTG12.0408163265306123No Hit
TAGTTATTTATTGCCCTATACAAGATACCCAATTGCTGTCTCTTATACACA12.0408163265306123No Hit
CAACATTAGTATTCAACACACGAAGAATGTGTTGAAACTCCTCATTTGCAA12.0408163265306123No Hit
ACTTCCTTGCTTCTCAAGGGGAAGAATTCAAGAAAGGATCCATGGATTCTC12.0408163265306123No Hit
GACACACATAGACACAAACACACAAATACATAGACACATGCACAAACATAC12.0408163265306123No Hit
ATGTCTTTCTTGGCTTTACAATGTATCGGAGATGTTAAGGTTAGACCATCG12.0408163265306123No Hit
CAACTGTAAGTAAGTACCTGAAGAGTGTTGGCAGATAGATAAAGAGTCTTC12.0408163265306123No Hit
CCTCCATTTTTCACCAAGTTTCCTCTGGTATACCGGAGAGGAAGAATCCTG12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)