##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_78.3410000000aade.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.62068965517241 0.0 0.0 0.0 0.0 0.0 2 37.206896551724135 0.0 0.0 0.0 0.0 0.0 3 37.41379310344828 0.0 0.0 0.0 0.0 0.0 4 36.724137931034484 0.0 0.0 0.0 0.0 0.0 5 37.13793103448276 0.0 0.0 0.0 0.0 0.0 6 38.93103448275862 0.0 0.0 0.0 0.0 0.0 7 38.86206896551724 0.0 0.0 0.0 0.0 0.0 8 38.275862068965516 0.0 0.0 0.0 0.0 0.0 9 37.93103448275862 0.0 0.0 0.0 0.0 0.0 10 38.58620689655172 0.0 0.0 0.0 0.0 0.0 11 38.172413793103445 0.0 0.0 0.0 0.0 0.0 12 37.827586206896555 0.0 0.0 0.0 0.0 0.0 13 38.06896551724138 0.0 0.0 0.0 0.0 0.0 14 37.48275862068966 0.0 0.0 0.0 0.0 0.0 15 37.51724137931034 0.0 0.0 0.0 0.0 0.0 16 37.41379310344828 0.0 0.0 0.0 0.0 0.0 17 37.96551724137931 0.0 0.0 0.0 0.0 0.0 18 38.03448275862069 0.0 0.0 0.0 0.0 0.0 19 37.86206896551724 0.0 0.0 0.0 0.0 0.0 20 37.758620689655174 0.0 0.0 0.0 0.0 0.0 21 39.3448275862069 0.0 0.0 0.0 0.0 0.0 22 38.6551724137931 0.0 0.0 0.0 0.0 0.0 23 38.172413793103445 0.0 0.0 0.0 0.0 0.0 24 38.689655172413794 0.0 0.0 0.0 0.0 0.0 25 38.41379310344828 0.0 0.0 0.0 0.0 0.0 26 38.724137931034484 0.0 0.0 0.0 0.0 0.0 27 38.96551724137931 0.0 0.0 0.0 0.0 0.0 28 38.6551724137931 0.0 0.0 0.0 0.0 0.0 29 37.793103448275865 0.0 0.0 0.0 0.0 0.0 30 38.51724137931034 0.0 0.0 0.0 0.0 0.0 31 38.03448275862069 0.0 0.0 0.0 0.0 0.0 32 38.3448275862069 0.0 0.0 0.0 0.0 0.0 33 38.275862068965516 0.0 0.0 0.0 0.0 0.0 34 37.41379310344828 0.0 0.0 0.0 0.0 0.0 35 37.96551724137931 0.0 0.0 0.0 0.0 0.0 36 38.37931034482759 0.0 0.0 0.0 0.0 0.0 37 37.793103448275865 0.0 0.0 0.0 0.0 0.0 38 38.48275862068966 0.0 0.0 0.0 0.0 0.0 39 38.0 0.0 0.0 0.0 0.0 0.0 40 38.48275862068966 0.0 0.0 0.0 0.0 0.0 41 38.89655172413793 0.0 0.0 0.0 0.0 0.0 42 39.10344827586207 0.0 0.0 0.0 0.0 0.0 43 38.62068965517241 0.0 0.0 0.0 0.0 0.0 44 38.13793103448276 0.0 0.0 0.0 0.0 0.0 45 39.10344827586207 0.0 0.0 0.0 0.0 0.0 46 39.06896551724138 0.0 0.0 0.0 0.0 0.0 47 38.86206896551724 0.0 0.0 0.0 0.0 0.0 48 38.51724137931034 0.0 0.0 0.0 0.0 0.0 49 39.48275862068966 0.0 0.0 0.0 0.0 0.0 50 38.51724137931034 0.0 0.0 0.0 0.0 0.0 51 38.58620689655172 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2114 1 0.0 2114 2 0.0 2114 3 0.0 2114 4 0.0 2114 5 0.0 2114 6 0.0 2114 7 -1.0 2114 8 0.0 2114 9 0.0 2114 10 0.0 2114 11 0.0 2114 12 0.0 2114 13 0.0 2114 14 0.0 2114 15 0.0 2114 16 0.0 2114 17 0.0 2114 18 0.0 2114 19 0.0 2114 20 0.0 2114 21 0.0 2114 22 0.0 2114 23 0.0 2114 24 0.0 2114 25 0.0 2114 26 0.0 2114 27 0.0 2114 28 0.0 2114 29 0.0 2114 30 0.0 2114 31 0.0 2114 32 0.0 2114 33 0.0 2114 34 0.0 2114 35 0.0 2114 36 0.0 2114 37 0.0 2114 38 0.0 2114 39 0.0 2114 40 0.0 2114 41 0.0 2114 42 0.0 2114 43 0.0 2114 44 0.0 2114 45 0.0 2114 46 0.0 2114 47 0.0 2114 48 1.0 2114 49 0.0 2114 50 0.0 2114 51 0.0 1204 1 0.0 1204 2 0.0 1204 3 0.0 1204 4 0.0 1204 5 0.0 1204 6 0.0 1204 7 1.0 1204 8 0.0 1204 9 0.0 1204 10 0.0 1204 11 0.0 1204 12 0.0 1204 13 0.0 1204 14 0.0 1204 15 0.0 1204 16 0.0 1204 17 0.0 1204 18 0.0 1204 19 0.0 1204 20 0.0 1204 21 0.0 1204 22 0.0 1204 23 0.0 1204 24 0.0 1204 25 0.0 1204 26 0.0 1204 27 0.0 1204 28 0.0 1204 29 0.0 1204 30 0.0 1204 31 0.0 1204 32 0.0 1204 33 0.0 1204 34 0.0 1204 35 0.0 1204 36 0.0 1204 37 0.0 1204 38 0.0 1204 39 0.0 1204 40 0.0 1204 41 0.0 1204 42 0.0 1204 43 0.0 1204 44 0.0 1204 45 0.0 1204 46 0.0 1204 47 0.0 1204 48 -1.0 1204 49 0.0 1204 50 0.0 1204 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 1.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 1.0 35 1.0 36 0.0 37 0.0 38 1.0 39 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.275862068965516 13.793103448275861 10.344827586206897 27.586206896551722 2 17.24137931034483 31.03448275862069 37.93103448275862 13.793103448275861 3 17.24137931034483 31.03448275862069 34.48275862068966 17.24137931034483 4 6.896551724137931 20.689655172413794 41.37931034482759 31.03448275862069 5 13.793103448275861 51.724137931034484 20.689655172413794 13.793103448275861 6 31.03448275862069 48.275862068965516 6.896551724137931 13.793103448275861 7 17.24137931034483 41.37931034482759 24.137931034482758 17.24137931034483 8 27.586206896551722 41.37931034482759 20.689655172413794 10.344827586206897 9 13.793103448275861 13.793103448275861 34.48275862068966 37.93103448275862 10 6.896551724137931 27.586206896551722 31.03448275862069 34.48275862068966 11 24.137931034482758 20.689655172413794 37.93103448275862 17.24137931034483 12 24.137931034482758 27.586206896551722 24.137931034482758 24.137931034482758 13 34.48275862068966 24.137931034482758 27.586206896551722 13.793103448275861 14 13.793103448275861 44.827586206896555 20.689655172413794 20.689655172413794 15 13.793103448275861 41.37931034482759 20.689655172413794 24.137931034482758 16 10.344827586206897 31.03448275862069 24.137931034482758 34.48275862068966 17 17.24137931034483 37.93103448275862 44.827586206896555 0.0 18 27.586206896551722 31.03448275862069 24.137931034482758 17.24137931034483 19 31.03448275862069 20.689655172413794 37.93103448275862 10.344827586206897 20 27.586206896551722 34.48275862068966 20.689655172413794 17.24137931034483 21 27.586206896551722 31.03448275862069 24.137931034482758 17.24137931034483 22 31.03448275862069 37.93103448275862 10.344827586206897 20.689655172413794 23 27.586206896551722 24.137931034482758 31.03448275862069 17.24137931034483 24 17.24137931034483 34.48275862068966 31.03448275862069 17.24137931034483 25 6.896551724137931 41.37931034482759 34.48275862068966 17.24137931034483 26 17.24137931034483 41.37931034482759 13.793103448275861 27.586206896551722 27 31.03448275862069 31.03448275862069 27.586206896551722 10.344827586206897 28 27.586206896551722 27.586206896551722 20.689655172413794 24.137931034482758 29 6.896551724137931 31.03448275862069 41.37931034482759 20.689655172413794 30 20.689655172413794 20.689655172413794 27.586206896551722 31.03448275862069 31 13.793103448275861 24.137931034482758 34.48275862068966 27.586206896551722 32 27.586206896551722 31.03448275862069 24.137931034482758 17.24137931034483 33 20.689655172413794 24.137931034482758 41.37931034482759 13.793103448275861 34 20.689655172413794 27.586206896551722 24.137931034482758 27.586206896551722 35 20.689655172413794 31.03448275862069 27.586206896551722 20.689655172413794 36 20.689655172413794 27.586206896551722 24.137931034482758 27.586206896551722 37 13.793103448275861 27.586206896551722 34.48275862068966 24.137931034482758 38 20.689655172413794 31.03448275862069 34.48275862068966 13.793103448275861 39 17.24137931034483 20.689655172413794 41.37931034482759 20.689655172413794 40 20.689655172413794 31.03448275862069 24.137931034482758 24.137931034482758 41 17.24137931034483 31.03448275862069 31.03448275862069 20.689655172413794 42 20.689655172413794 24.137931034482758 24.137931034482758 31.03448275862069 43 10.344827586206897 31.03448275862069 41.37931034482759 17.24137931034483 44 10.344827586206897 27.586206896551722 48.275862068965516 13.793103448275861 45 10.344827586206897 24.137931034482758 34.48275862068966 31.03448275862069 46 3.4482758620689653 24.137931034482758 48.275862068965516 24.137931034482758 47 27.586206896551722 24.137931034482758 24.137931034482758 24.137931034482758 48 17.24137931034483 27.586206896551722 34.48275862068966 20.689655172413794 49 17.24137931034483 27.586206896551722 31.03448275862069 24.137931034482758 50 10.344827586206897 34.48275862068966 34.48275862068966 20.689655172413794 51 6.896551724137931 31.03448275862069 20.689655172413794 41.37931034482759 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 2.0 33 4.0 34 3.5 35 3.0 36 3.5 37 4.0 38 6.0 39 8.0 40 5.0 41 2.0 42 2.5 43 3.0 44 2.5 45 2.0 46 1.5 47 1.0 48 0.5 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.5 57 1.0 58 0.5 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 29.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACGGGGACTTCATTTATCTCTCTCTCTCTCTATTTCTCTGTCTCTTATA 1 3.4482758620689653 No Hit AAGTACCTTCGTGCAATAGATCGAAAGCCTTATTGATTTCTCCCAAGGTCA 1 3.4482758620689653 No Hit GGATGGTTACTCTGGTGTTGAGGTTAGGGTTACTCCTATGAGGACTGAGAT 1 3.4482758620689653 No Hit TTTTCACGTAGGAGACAGGAGTGGACATTATCTGAATTACCGATAATATCC 1 3.4482758620689653 No Hit CAACCAAGGCAACGGCTCAGCCGGCAGCCCCCGCTCCAGAGCCTGCTGGTC 1 3.4482758620689653 No Hit GTCCAAAGCAAGCTTGAAGAACCAAATGTCGATAATGTCTGTCTCTTATAC 1 3.4482758620689653 No Hit CAACAACATTGTAACCGTTGCAACACTTAACAACATCACAATTTCTGTCTC 1 3.4482758620689653 No Hit AGTATGAACTGATCAATGTTGGTGGTGATCAGTGATCTTTTAGTATGTTTA 1 3.4482758620689653 No Hit ATATCAAAGTGGTTTAACCTTGGTTATCTACAAATCAGTGGATGTTGCTGT 1 3.4482758620689653 No Hit GGTTTAAGTATCGAAGTTTTGGTAGGGAACGCTGGTTCCTCTTTCTGTCTC 1 3.4482758620689653 No Hit TCCCTATGTATTCAGAATACGATGACCTCCCGTACCTGTCTCTTATACACA 1 3.4482758620689653 No Hit CTCAACAATGATAACCTGGAGCACAAGAGTTATGACTATTATTCCCATAAA 1 3.4482758620689653 No Hit CAATAGATCTTGTAACTGTAGGAATCTTTTGTCTGGATGGTAAAACGATCA 1 3.4482758620689653 No Hit CTTAACGCCAACACCCAACACATCCAACAATTGCCAATCAAAAACACAGCC 1 3.4482758620689653 No Hit GCTAGATTCTACTCTTACGGTAAATGTATATTCACCCATACCATTCCTGTC 1 3.4482758620689653 No Hit ACGTAATACATCTTCGTAATGAAAATTGAGTGGAAACATGAGTTGGCTTTT 1 3.4482758620689653 No Hit GAGTAGTTCCCAGATAAGGGAATTAGGGTTCCTATAGGGCTGTCTCTTATA 1 3.4482758620689653 No Hit GTTCAGAGAAGAGAACATGGTGATAGAGAGATGTCTAAAAACCAATAGTGC 1 3.4482758620689653 No Hit TACCGACTCCTCTGATGAGAAACTTTGAAGATTGGCCATAAAGTTTCACAC 1 3.4482758620689653 No Hit CATGTAAAGGGGAAATAAAACAGTTTGATGAGACAGAGTAAGCATTAGGAC 1 3.4482758620689653 No Hit GGAATGGCATCAGATATATATCCCCAACAGGAAGAATACTCCTTTTGTCAA 1 3.4482758620689653 No Hit CTACAGTATCTTGTCCGTTCAATTTCTGTCTGATTGATGGCCTTTTTTCGT 1 3.4482758620689653 No Hit CTTTTAAGTTTCGTACGAGATGTAACCCCTATGCCTGTCTCTTATACACAT 1 3.4482758620689653 No Hit GAATACCACCTGATCAACAGAGGTTGATATTTGCTGGTAAGCAGCTTGAAG 1 3.4482758620689653 No Hit GTTAATGCCATGACAATGACAACAAAATCAAGAAGACTAGTTAAGACACTA 1 3.4482758620689653 No Hit GTGGAAGATCCTGACTTGTTGGAACAAATCCGATTAACAATCATCAACAAT 1 3.4482758620689653 No Hit GAGTAAAATTCAGAGTTTTCATTTCACAAGCATCTGTCTCTTATACACATC 1 3.4482758620689653 No Hit GGCTCGTACCAAGCAAACCGCTCGTAAGTCCACCGGAGGTAAAGCTCCAAG 1 3.4482758620689653 No Hit GCTCATAAGTCATAACTGTTAAGAACAGCTAGTTACTGACGATATAACTCC 1 3.4482758620689653 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 3.4482758620689653 35 0.0 0.0 6.896551724137931 36 0.0 0.0 10.344827586206897 37 0.0 0.0 10.344827586206897 38 0.0 0.0 10.344827586206897 39 0.0 0.0 13.793103448275861 >>END_MODULE >>Kmer Content pass >>END_MODULE