FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_78.3410000000aade.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_78.3410000000aade.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGGACTTCATTTATCTCTCTCTCTCTCTATTTCTCTGTCTCTTATA13.4482758620689653No Hit
AAGTACCTTCGTGCAATAGATCGAAAGCCTTATTGATTTCTCCCAAGGTCA13.4482758620689653No Hit
GGATGGTTACTCTGGTGTTGAGGTTAGGGTTACTCCTATGAGGACTGAGAT13.4482758620689653No Hit
TTTTCACGTAGGAGACAGGAGTGGACATTATCTGAATTACCGATAATATCC13.4482758620689653No Hit
CAACCAAGGCAACGGCTCAGCCGGCAGCCCCCGCTCCAGAGCCTGCTGGTC13.4482758620689653No Hit
GTCCAAAGCAAGCTTGAAGAACCAAATGTCGATAATGTCTGTCTCTTATAC13.4482758620689653No Hit
CAACAACATTGTAACCGTTGCAACACTTAACAACATCACAATTTCTGTCTC13.4482758620689653No Hit
AGTATGAACTGATCAATGTTGGTGGTGATCAGTGATCTTTTAGTATGTTTA13.4482758620689653No Hit
ATATCAAAGTGGTTTAACCTTGGTTATCTACAAATCAGTGGATGTTGCTGT13.4482758620689653No Hit
GGTTTAAGTATCGAAGTTTTGGTAGGGAACGCTGGTTCCTCTTTCTGTCTC13.4482758620689653No Hit
TCCCTATGTATTCAGAATACGATGACCTCCCGTACCTGTCTCTTATACACA13.4482758620689653No Hit
CTCAACAATGATAACCTGGAGCACAAGAGTTATGACTATTATTCCCATAAA13.4482758620689653No Hit
CAATAGATCTTGTAACTGTAGGAATCTTTTGTCTGGATGGTAAAACGATCA13.4482758620689653No Hit
CTTAACGCCAACACCCAACACATCCAACAATTGCCAATCAAAAACACAGCC13.4482758620689653No Hit
GCTAGATTCTACTCTTACGGTAAATGTATATTCACCCATACCATTCCTGTC13.4482758620689653No Hit
ACGTAATACATCTTCGTAATGAAAATTGAGTGGAAACATGAGTTGGCTTTT13.4482758620689653No Hit
GAGTAGTTCCCAGATAAGGGAATTAGGGTTCCTATAGGGCTGTCTCTTATA13.4482758620689653No Hit
GTTCAGAGAAGAGAACATGGTGATAGAGAGATGTCTAAAAACCAATAGTGC13.4482758620689653No Hit
TACCGACTCCTCTGATGAGAAACTTTGAAGATTGGCCATAAAGTTTCACAC13.4482758620689653No Hit
CATGTAAAGGGGAAATAAAACAGTTTGATGAGACAGAGTAAGCATTAGGAC13.4482758620689653No Hit
GGAATGGCATCAGATATATATCCCCAACAGGAAGAATACTCCTTTTGTCAA13.4482758620689653No Hit
CTACAGTATCTTGTCCGTTCAATTTCTGTCTGATTGATGGCCTTTTTTCGT13.4482758620689653No Hit
CTTTTAAGTTTCGTACGAGATGTAACCCCTATGCCTGTCTCTTATACACAT13.4482758620689653No Hit
GAATACCACCTGATCAACAGAGGTTGATATTTGCTGGTAAGCAGCTTGAAG13.4482758620689653No Hit
GTTAATGCCATGACAATGACAACAAAATCAAGAAGACTAGTTAAGACACTA13.4482758620689653No Hit
GTGGAAGATCCTGACTTGTTGGAACAAATCCGATTAACAATCATCAACAAT13.4482758620689653No Hit
GAGTAAAATTCAGAGTTTTCATTTCACAAGCATCTGTCTCTTATACACATC13.4482758620689653No Hit
GGCTCGTACCAAGCAAACCGCTCGTAAGTCCACCGGAGGTAAAGCTCCAAG13.4482758620689653No Hit
GCTCATAAGTCATAACTGTTAAGAACAGCTAGTTACTGACGATATAACTCC13.4482758620689653No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers