FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_77.3410000000aac1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_77.3410000000aac1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC23.8461538461538463No Hit
GGTTAACGATTGGGCAGAAGAACTTGTTCCTCAGCTTGTGGAAATACCTCA11.9230769230769231No Hit
AGGTCATTCCTTCGACCATGAATCCTGGTTTGGGTCCTTTAGGCTGTCTCT11.9230769230769231No Hit
GTTTCACATTGATCTTCAGCAACTTCATTCTTCTTCTTCTTCTTCTTCTTC11.9230769230769231No Hit
GTCCCTGGATCATTTCAATATTCATGACAAGTTGATGTTACTGTCGTAAAT11.9230769230769231No Hit
GGATAGGTCTCTGTAAATTGTTGTTAGATACAAAAAGAGAGATAGGGTTTT11.9230769230769231No Hit
TCTTAGCAGAGAAGCTGTCGTCGATAGAGTACTCGATCTGTCTCTTATACA11.9230769230769231No Hit
AGAATGATCAACACAGTTCCTGGAGTGATGCTGGCTTTTAGCTTGGTAGGC11.9230769230769231No Hit
CTCTTGATTATTTCGTTATGATTTTTGTGGCAATCTTCTTGCTGTGTTTGT11.9230769230769231No Hit
GACGTCACACTGCCAAGAAAAGCTTCTAGCGTTTAAACATACTCTACCCGT11.9230769230769231No Hit
TTTGACAGAGTGAAACCCTTTCTTCAACACACCTGACAGTCTGTCTCTTAT11.9230769230769231No Hit
ACATCAAGGAACTCGCTTCTGCGCACAGAGTTGCTCCTGTCTCTTATACAC11.9230769230769231No Hit
TATCAACGCAGAGTACGGGAACTGACAATTAACAGCCCAATATCTACCTGT11.9230769230769231No Hit
TCCTAAATCCTGACTCGGGCTCCTTCCACAAGACCTGTCTCTTATACACAT11.9230769230769231No Hit
GAGTACGGGGACCTTCTTCGGTGAAGAATTAAGCAGTAGATCCTGTCTCTT11.9230769230769231No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACCC11.9230769230769231No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAACCTATCTCGTATGCCGTC11.9230769230769231No Hit
TATCAACGCAGAGTACGGGGAGGGACTTTCACTCCTGTCTCTTATACACAT11.9230769230769231No Hit
ATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACTCCTGTC11.9230769230769231No Hit
GCTTACTACATTCAGAGAGGTAGTTCCTTTTTTTTTTTGAACATGTGCAAC11.9230769230769231No Hit
NTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC11.9230769230769231No Hit
ACTCAGAACCAAATCCCAGAAATGCAAAGATAATGTGCTTTGGTAATGATG11.9230769230769231No Hit
CTACACAAGCATTTGCGAGAAAGAAGAGAATAAAGGGACTGTCTCTTATAC11.9230769230769231No Hit
TGACAAGATCATCAGTAGACATATCGAGAAGAGCATCGAGATCAACTCCTT11.9230769230769231No Hit
ATTTTCACCCTCTACGATGTCCTATTCCAAGGAACTTGTCCATAGCTGTCT11.9230769230769231No Hit
ATCATGGTCAGCTTCTAAGCAGTTACAAGCTTTAGCGAGTAACTTGGCAAC11.9230769230769231No Hit
TCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCTGTC11.9230769230769231No Hit
ACTAAAAGTCTCAGGAAAGTCTTATTGTTGATAACCAGATTGATACTGCAA11.9230769230769231No Hit
GACTCGGGTGGTGACCCAGACTGGCACGAGGAAACCGAGCACAAACGCCTG11.9230769230769231No Hit
CCTCCAATTATGTCACCAAGTAATTCAGTGTTCTCATCAAGCAGCTTGGTT11.9230769230769231No Hit
GACAGCTCACCGTCCAGGGCGCTGGAGACTTGGCTCAGGATCGCCATGCCA11.9230769230769231No Hit
GATGGAAAAGCAAATGCAGGTATAAGTAACGATAAAGGCTGTCTCTTATAC11.9230769230769231No Hit
GTGTGTCCGTGAAGGGCTACACTGCCGACTCGAAGGGCGCCCTCGACTGCA11.9230769230769231No Hit
GTACGGGGATCCATTCATTCACCTTTTCTGTGAGCAGGCACCATGGCAGGC11.9230769230769231No Hit
GTCTTAACTAGGGAATGGGAAGCTTTCACTGGCATTTAGTACCCTGTCTCT11.9230769230769231No Hit
GTGCAAGACCTGGGTCTTGCTGAAGCAACTCTCTCAGGTTGTTCTCCACCT11.9230769230769231No Hit
TTCCTTTACCATCCACGAAGCACGAAGAAGATTCAAACTGAACTACCTGTC11.9230769230769231No Hit
TAATAAGGACAATCTGTGGGTGCCAAATAAGGTGTTATCATTTTACCAGTA11.9230769230769231No Hit
GATCAATAATCTCTTACCGGCGATGAGGAATCTTAACAACGCTTTGGTTCG11.9230769230769231No Hit
CAATAAAACCTTAAACAAGTAATCAAATTCGGGTTGTCCTGACGAAGAACC11.9230769230769231No Hit
GCAGATTCCTCATAGTTTTCACTCTCTATCGAATTGATCTTAGCTTCGTTG11.9230769230769231No Hit
ATGTAAGCTTTCCCAATCGGACCAACCAAGTAATCAGTGAACGCTGTCTCT11.9230769230769231No Hit
GCAGAAGGTTTTACAGTGATATTCAACAAAGCTAGGGATGAGAAAAAGACC11.9230769230769231No Hit
CTAAAATGGCTGCAGAGAACAGGGAGAAAGTCGCTCAGCAAATGGACTGTC11.9230769230769231No Hit
CCATTACATTCGGATTGATTCTATTCAACTCCCTTACTCCTGTCTCTTATA11.9230769230769231No Hit
AAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCCTGTCTC11.9230769230769231No Hit
GTAGTAGACAATGAATAAAGTGATAACGCTAACACACACTGCATGCCCTGT11.9230769230769231No Hit
AGTTCTGGTACCTCTTGAGGAAGCTGAAGAAAGTGAAGAAAAGTAATGGCC11.9230769230769231No Hit
CTTGTTTCTTCCTCGATCTCTCGATCTTCTGCTTTTGCTTTTCCGATTAAG11.9230769230769231No Hit
CTACATAACGGATGAAATGCTGAGAGGTGTGGATTCTGTTGTGTAGCTGTC11.9230769230769231No Hit
GTACTACATCAATCCTGCTACAAGTCAGCCTAATGTGTCTGGACCTGTCTC11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATC50.045.04
GTAGACA50.045.015
GAGAAGA50.045.026
AGAAGAG50.045.027
AGTAGAC50.045.014
CAGTAGA50.045.013
CAACTCC50.045.043
AAGAGCA50.045.029
TATCGAG50.045.022
ACTCCTT50.045.045
TGACAAG50.045.01
TCAACTC50.045.042
CATCAGT50.045.010
GAAGAGC50.045.028
ATCAGTA50.045.011
GAGCATC50.045.031
GACATAT50.045.018
AACTCCT50.045.044
TCAGTAG50.045.012
ACATATC50.045.019
Produced by FastQC (version 0.11.2)