FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_76.3410000000aab4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_76.3410000000aab4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGGGAGCAGCCGCGCTTTTTGGCCGAGGAAGGATAAAGAGCTGTCT12.3255813953488373No Hit
GACGTAGACACTCATAAAGGTCAAATCATACCAACATTGCACCAGAGGCTG12.3255813953488373No Hit
CCTTTCCACTGATTGCATCTGATGTCTTTTGAAGAGAGCTAGTCCCTGTCT12.3255813953488373No Hit
GTTGAAGTGAATGAGAAAAAAGACAGAGTTACTGATGCTCTCAATGCTACA12.3255813953488373No Hit
GGACGTTAAGGCAAATGAGATCGGGCAATACACAGAACGCACACTTCCAAC12.3255813953488373No Hit
CTTCCACATAGTAGTTCTAGCAGAACAACCCCGAAACCTGTCTCTTATACA12.3255813953488373No Hit
TTTTTGCGTTGATACCACTGCCCCGTACTCTGCGTTGATACCTGTCTCTTA12.3255813953488373No Hit
GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCT12.3255813953488373No Hit
GTCTCCACATTGAACCCAATAGTGGGAATGGTGGTTACAATCTCTCCGAGC12.3255813953488373No Hit
TCGTTACGCTCCTGAGGATCCGAATCTCCCTAAGCCATGGAAAGGTCTTGT12.3255813953488373No Hit
GGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAA12.3255813953488373No Hit
GCACATGCTGCCACCTTAGCAACAGCCCGCAGGATCGCAGCTCTTACCGCT12.3255813953488373No Hit
CATTTATGGTGTGACGGCGTACCTTTTGTATAATGGGTCAGCGACTTATAT12.3255813953488373No Hit
GTACCAGAGTAGAACTGGGTACCACAACATGTACAAGGCTGTCTCTTATAC12.3255813953488373No Hit
GAGCTAAGTGGAAGAAGAAGCGTATGAGGAGATTGAAGAGGAAGAGACGAA12.3255813953488373No Hit
GAGTACGGGGGATCGGAGTAATGATTAACAGGGACAGTCGGGCTGTCTCTT12.3255813953488373No Hit
CAACATACTCAAACTCGCCAATGTAACCGTGCTTTTGCATGACGATAAGGA12.3255813953488373No Hit
CTTAATGACAGACTCAGAAGCCAAGAAAGCATGGTAAGACTTAGCAAGCTT12.3255813953488373No Hit
GTCTTAATCACATCCATTGTTTGAAGAATCAAGTAATCTGATTTCTCCGGA12.3255813953488373No Hit
CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCAC12.3255813953488373No Hit
GATTGGTTGGCTTCGATGAAGATCGTCAAGCTACGAACATTCTGAAACTTT12.3255813953488373No Hit
CTCTATGAGGGCGGTAAGAGGAAGATAATCGAAAAGGTCTGTAAAATCTGT12.3255813953488373No Hit
CAGTAGGCTAACTAAAGCAGACGATTGGAAGTGTCTGTCTAAGCAGTGAGG12.3255813953488373No Hit
GTGTACATACCTTCGGCGGCGACGAAGAGCTCCTTTTGTTTCTTGAAACGG12.3255813953488373No Hit
GCTCTGAAACTCCCTAGGAAACGTTTTGCTATAGGTGGAAAACTTCTGTTC12.3255813953488373No Hit
GTCTTCCTCTTGTCCTGATCATCAGGAACAGGTTGAAGTATGCTTTGACTT12.3255813953488373No Hit
GTTTTTAATAAACAGTTGCAGCGGCCTGGTATCTGCGACTGCCAATAGCTT12.3255813953488373No Hit
GTATAATTACTGGTTCTTTTAAGATTGACATGAGAAGCATTGATGGGCAGT12.3255813953488373No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACCTGTCTCTTATACACA12.3255813953488373No Hit
CTCACCCACACACCCAGGGCTTTCCGGCTGATAACCTCGGTCTATCAGCTG12.3255813953488373No Hit
CATCACTATTCTCCGAGACGAATCTCTCTTCGTGTAAATCGACGATCCTTC12.3255813953488373No Hit
CAAGCATAACAAGGACACAGTTGAGTCTAGCGGAGAAGCTAAACACAGCTG12.3255813953488373No Hit
CACTTGAGGTCTTCATGTGCAGCATTGTCCGTAAAATGGGTTATGGCTGTC12.3255813953488373No Hit
CATTTAACTAAAACCCCTACGCACTTATATAGAGGAGACAAGCTGTCTCTT12.3255813953488373No Hit
CTTTCTACAGAGATTGATATGATCAAGTTAGTTGGACAGTTCTACTTTGCT12.3255813953488373No Hit
GTAACACACTGCGTTTACGGCGCTATCTTTAACGACAATACGTTTATACCC12.3255813953488373No Hit
GTCTTTTATGTCTTCAACGTTTTCACTTAGGATTTTGTTCTTTGGGGTAAG12.3255813953488373No Hit
CTCCGTATCCTCATGAACTACACTTCTCCTTCTCCCCATGATTCTACTAAA12.3255813953488373No Hit
GGGTAGGATGCAAGACCCGCACCATTGGTAATAACCCATCCAAGAGCGAAA12.3255813953488373No Hit
ATGGACTACTCAATGTCGAAGATGTGAAGAAATGGAAAATGATAGATGACT12.3255813953488373No Hit
TCTCATACCTCTGATACTTCTTCACAAAGTGAAGGTAATCCCTTCGCACGA12.3255813953488373No Hit
GAACAATGTTGCTGAAGGATGTGTTGGTCGTGTTGCTGCTAAGCTTGAGAT12.3255813953488373No Hit
ATCGTCGATACTCCCTCAAGTATTGACAATCGGATTAAGCTCATTGAAGAA12.3255813953488373No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)