##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_76.3410000000aab4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 43 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.86046511627907 0.0 0.0 0.0 0.0 0.0 2 37.74418604651163 0.0 0.0 0.0 0.0 0.0 3 38.0 0.0 0.0 0.0 0.0 0.0 4 38.0 0.0 0.0 0.0 0.0 0.0 5 38.0 0.0 0.0 0.0 0.0 0.0 6 39.81395348837209 0.0 0.0 0.0 0.0 0.0 7 39.95348837209303 0.0 0.0 0.0 0.0 0.0 8 39.81395348837209 0.0 0.0 0.0 0.0 0.0 9 39.76744186046512 0.0 0.0 0.0 0.0 0.0 10 39.81395348837209 0.0 0.0 0.0 0.0 0.0 11 39.95348837209303 0.0 0.0 0.0 0.0 0.0 12 40.0 0.0 0.0 0.0 0.0 0.0 13 39.906976744186046 0.0 0.0 0.0 0.0 0.0 14 39.906976744186046 0.0 0.0 0.0 0.0 0.0 15 39.81395348837209 0.0 0.0 0.0 0.0 0.0 16 39.86046511627907 0.0 0.0 0.0 0.0 0.0 17 39.95348837209303 0.0 0.0 0.0 0.0 0.0 18 39.86046511627907 0.0 0.0 0.0 0.0 0.0 19 39.906976744186046 0.0 0.0 0.0 0.0 0.0 20 39.86046511627907 0.0 0.0 0.0 0.0 0.0 21 39.86046511627907 0.0 0.0 0.0 0.0 0.0 22 39.906976744186046 0.0 0.0 0.0 0.0 0.0 23 39.95348837209303 0.0 0.0 0.0 0.0 0.0 24 39.906976744186046 0.0 0.0 0.0 0.0 0.0 25 40.0 0.0 0.0 0.0 0.0 0.0 26 39.906976744186046 0.0 0.0 0.0 0.0 0.0 27 40.0 0.0 0.0 0.0 0.0 0.0 28 40.0 0.0 0.0 0.0 0.0 0.0 29 39.95348837209303 0.0 0.0 0.0 0.0 0.0 30 39.86046511627907 0.0 0.0 0.0 0.0 0.0 31 40.0 0.0 0.0 0.0 0.0 0.0 32 39.86046511627907 0.0 0.0 0.0 0.0 0.0 33 40.0 0.0 0.0 0.0 0.0 0.0 34 39.95348837209303 0.0 0.0 0.0 0.0 0.0 35 39.95348837209303 0.0 0.0 0.0 0.0 0.0 36 40.0 0.0 0.0 0.0 0.0 0.0 37 39.906976744186046 0.0 0.0 0.0 0.0 0.0 38 39.95348837209303 0.0 0.0 0.0 0.0 0.0 39 40.0 0.0 0.0 0.0 0.0 0.0 40 39.81395348837209 0.0 0.0 0.0 0.0 0.0 41 39.81395348837209 0.0 0.0 0.0 0.0 0.0 42 39.906976744186046 0.0 0.0 0.0 0.0 0.0 43 39.81395348837209 0.0 0.0 0.0 0.0 0.0 44 39.906976744186046 0.0 0.0 0.0 0.0 0.0 45 39.53488372093023 0.0 0.0 0.0 0.0 0.0 46 39.627906976744185 0.0 0.0 0.0 0.0 0.0 47 39.81395348837209 0.0 0.0 0.0 0.0 0.0 48 39.81395348837209 0.0 0.0 0.0 0.0 0.0 49 39.76744186046512 0.0 0.0 0.0 0.0 0.0 50 39.86046511627907 0.0 0.0 0.0 0.0 0.0 51 39.674418604651166 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2101 1 0.0 2101 2 0.0 2101 3 0.0 2101 4 0.0 2101 5 0.0 2101 6 -1.5 2101 7 0.0 2101 8 0.0 2101 9 0.5 2101 10 0.5 2101 11 0.0 2101 12 0.0 2101 13 0.0 2101 14 0.5 2101 15 0.0 2101 16 0.0 2101 17 0.0 2101 18 0.0 2101 19 0.0 2101 20 -1.5 2101 21 0.0 2101 22 0.0 2101 23 0.0 2101 24 0.0 2101 25 0.0 2101 26 0.5 2101 27 0.0 2101 28 0.0 2101 29 0.0 2101 30 0.0 2101 31 0.0 2101 32 0.0 2101 33 0.0 2101 34 0.0 2101 35 0.0 2101 36 0.0 2101 37 0.0 2101 38 0.0 2101 39 0.0 2101 40 0.0 2101 41 0.0 2101 42 0.0 2101 43 0.0 2101 44 0.0 2101 45 0.5 2101 46 0.5 2101 47 0.0 2101 48 1.0 2101 49 0.0 2101 50 0.0 2101 51 0.0 1115 1 0.0 1115 2 0.0 1115 3 0.0 1115 4 0.0 1115 5 0.0 1115 6 0.5 1115 7 0.0 1115 8 0.0 1115 9 0.5 1115 10 0.5 1115 11 0.0 1115 12 0.0 1115 13 0.0 1115 14 0.5 1115 15 0.0 1115 16 0.0 1115 17 0.0 1115 18 0.0 1115 19 0.0 1115 20 0.5 1115 21 0.0 1115 22 0.0 1115 23 0.0 1115 24 0.0 1115 25 0.0 1115 26 0.5 1115 27 0.0 1115 28 0.0 1115 29 0.0 1115 30 0.0 1115 31 0.0 1115 32 0.0 1115 33 0.0 1115 34 0.0 1115 35 0.0 1115 36 0.0 1115 37 0.0 1115 38 0.0 1115 39 0.0 1115 40 0.0 1115 41 0.0 1115 42 0.0 1115 43 0.0 1115 44 0.0 1115 45 0.5 1115 46 0.5 1115 47 0.0 1115 48 1.0 1115 49 0.0 1115 50 0.0 1115 51 0.0 2115 1 0.0 2115 2 0.0 2115 3 0.0 2115 4 0.0 2115 5 0.0 2115 6 0.5 2115 7 0.0 2115 8 0.0 2115 9 -1.5 2115 10 -1.5 2115 11 0.0 2115 12 0.0 2115 13 0.0 2115 14 -1.5 2115 15 0.0 2115 16 0.0 2115 17 0.0 2115 18 0.0 2115 19 0.0 2115 20 0.5 2115 21 0.0 2115 22 0.0 2115 23 0.0 2115 24 0.0 2115 25 0.0 2115 26 -1.5 2115 27 0.0 2115 28 0.0 2115 29 0.0 2115 30 0.0 2115 31 0.0 2115 32 0.0 2115 33 0.0 2115 34 0.0 2115 35 0.0 2115 36 0.0 2115 37 0.0 2115 38 0.0 2115 39 0.0 2115 40 0.0 2115 41 0.0 2115 42 0.0 2115 43 0.0 2115 44 0.0 2115 45 -1.5 2115 46 -1.5 2115 47 0.0 2115 48 -1.0 2115 49 0.0 2115 50 0.0 2115 51 0.0 2212 1 0.0 2212 2 0.0 2212 3 0.0 2212 4 0.0 2212 5 0.0 2212 6 0.5 2212 7 0.0 2212 8 0.0 2212 9 0.5 2212 10 0.5 2212 11 0.0 2212 12 0.0 2212 13 0.0 2212 14 0.5 2212 15 0.0 2212 16 0.0 2212 17 0.0 2212 18 0.0 2212 19 0.0 2212 20 0.5 2212 21 0.0 2212 22 0.0 2212 23 0.0 2212 24 0.0 2212 25 0.0 2212 26 0.5 2212 27 0.0 2212 28 0.0 2212 29 0.0 2212 30 0.0 2212 31 0.0 2212 32 0.0 2212 33 0.0 2212 34 0.0 2212 35 0.0 2212 36 0.0 2212 37 0.0 2212 38 0.0 2212 39 0.0 2212 40 0.0 2212 41 0.0 2212 42 0.0 2212 43 0.0 2212 44 0.0 2212 45 0.5 2212 46 0.5 2212 47 0.0 2212 48 -1.0 2212 49 0.0 2212 50 0.0 2212 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 38 1.0 39 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.162790697674424 4.651162790697675 6.976744186046512 37.2093023255814 2 6.976744186046512 30.23255813953488 48.837209302325576 13.953488372093023 3 16.27906976744186 20.930232558139537 34.883720930232556 27.906976744186046 4 11.627906976744185 13.953488372093023 44.18604651162791 30.23255813953488 5 11.627906976744185 34.883720930232556 32.55813953488372 20.930232558139537 6 18.6046511627907 32.55813953488372 25.581395348837212 23.25581395348837 7 27.906976744186046 34.883720930232556 20.930232558139537 16.27906976744186 8 20.930232558139537 39.53488372093023 18.6046511627907 20.930232558139537 9 20.930232558139537 16.27906976744186 30.23255813953488 32.55813953488372 10 20.930232558139537 32.55813953488372 30.23255813953488 16.27906976744186 11 30.23255813953488 23.25581395348837 16.27906976744186 30.23255813953488 12 13.953488372093023 32.55813953488372 25.581395348837212 27.906976744186046 13 16.27906976744186 34.883720930232556 27.906976744186046 20.930232558139537 14 20.930232558139537 25.581395348837212 18.6046511627907 34.883720930232556 15 25.581395348837212 20.930232558139537 23.25581395348837 30.23255813953488 16 16.27906976744186 39.53488372093023 23.25581395348837 20.930232558139537 17 39.53488372093023 27.906976744186046 11.627906976744185 20.930232558139537 18 27.906976744186046 27.906976744186046 20.930232558139537 23.25581395348837 19 34.883720930232556 34.883720930232556 18.6046511627907 11.627906976744185 20 27.906976744186046 23.25581395348837 23.25581395348837 25.581395348837212 21 23.25581395348837 32.55813953488372 25.581395348837212 18.6046511627907 22 13.953488372093023 30.23255813953488 30.23255813953488 25.581395348837212 23 30.23255813953488 18.6046511627907 25.581395348837212 25.581395348837212 24 18.6046511627907 39.53488372093023 20.930232558139537 20.930232558139537 25 23.25581395348837 23.25581395348837 44.18604651162791 9.30232558139535 26 23.25581395348837 16.27906976744186 34.883720930232556 25.581395348837212 27 25.581395348837212 37.2093023255814 18.6046511627907 18.6046511627907 28 18.6046511627907 39.53488372093023 20.930232558139537 20.930232558139537 29 18.6046511627907 18.6046511627907 39.53488372093023 23.25581395348837 30 16.27906976744186 32.55813953488372 30.23255813953488 20.930232558139537 31 30.23255813953488 34.883720930232556 16.27906976744186 18.6046511627907 32 27.906976744186046 30.23255813953488 27.906976744186046 13.953488372093023 33 25.581395348837212 34.883720930232556 25.581395348837212 13.953488372093023 34 34.883720930232556 32.55813953488372 25.581395348837212 6.976744186046512 35 34.883720930232556 23.25581395348837 23.25581395348837 18.6046511627907 36 9.30232558139535 41.86046511627907 25.581395348837212 23.25581395348837 37 37.2093023255814 34.883720930232556 16.27906976744186 11.627906976744185 38 27.906976744186046 27.906976744186046 18.6046511627907 25.581395348837212 39 16.27906976744186 23.25581395348837 34.883720930232556 25.581395348837212 40 23.25581395348837 20.930232558139537 32.55813953488372 23.25581395348837 41 20.930232558139537 30.23255813953488 34.883720930232556 13.953488372093023 42 18.6046511627907 25.581395348837212 18.6046511627907 37.2093023255814 43 9.30232558139535 30.23255813953488 37.2093023255814 23.25581395348837 44 20.930232558139537 20.930232558139537 37.2093023255814 20.930232558139537 45 27.906976744186046 23.25581395348837 30.23255813953488 18.6046511627907 46 25.581395348837212 23.25581395348837 34.883720930232556 16.27906976744186 47 13.953488372093023 25.581395348837212 27.906976744186046 32.55813953488372 48 30.23255813953488 20.930232558139537 20.930232558139537 27.906976744186046 49 18.6046511627907 27.906976744186046 23.25581395348837 30.23255813953488 50 20.930232558139537 25.581395348837212 30.23255813953488 23.25581395348837 51 13.953488372093023 27.906976744186046 39.53488372093023 18.6046511627907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 2.5 35 5.0 36 3.0 37 1.0 38 1.5 39 2.0 40 3.0 41 4.0 42 6.0 43 8.0 44 8.0 45 8.0 46 6.5 47 5.0 48 3.5 49 2.0 50 2.5 51 3.0 52 2.0 53 1.0 54 0.5 55 0.0 56 0.0 57 0.0 58 1.5 59 3.0 60 2.0 61 1.0 62 0.5 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 43.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACGGGGGAGCAGCCGCGCTTTTTGGCCGAGGAAGGATAAAGAGCTGTCT 1 2.3255813953488373 No Hit GACGTAGACACTCATAAAGGTCAAATCATACCAACATTGCACCAGAGGCTG 1 2.3255813953488373 No Hit CCTTTCCACTGATTGCATCTGATGTCTTTTGAAGAGAGCTAGTCCCTGTCT 1 2.3255813953488373 No Hit GTTGAAGTGAATGAGAAAAAAGACAGAGTTACTGATGCTCTCAATGCTACA 1 2.3255813953488373 No Hit GGACGTTAAGGCAAATGAGATCGGGCAATACACAGAACGCACACTTCCAAC 1 2.3255813953488373 No Hit CTTCCACATAGTAGTTCTAGCAGAACAACCCCGAAACCTGTCTCTTATACA 1 2.3255813953488373 No Hit TTTTTGCGTTGATACCACTGCCCCGTACTCTGCGTTGATACCTGTCTCTTA 1 2.3255813953488373 No Hit GACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCT 1 2.3255813953488373 No Hit GTCTCCACATTGAACCCAATAGTGGGAATGGTGGTTACAATCTCTCCGAGC 1 2.3255813953488373 No Hit TCGTTACGCTCCTGAGGATCCGAATCTCCCTAAGCCATGGAAAGGTCTTGT 1 2.3255813953488373 No Hit GGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAA 1 2.3255813953488373 No Hit GCACATGCTGCCACCTTAGCAACAGCCCGCAGGATCGCAGCTCTTACCGCT 1 2.3255813953488373 No Hit CATTTATGGTGTGACGGCGTACCTTTTGTATAATGGGTCAGCGACTTATAT 1 2.3255813953488373 No Hit GTACCAGAGTAGAACTGGGTACCACAACATGTACAAGGCTGTCTCTTATAC 1 2.3255813953488373 No Hit GAGCTAAGTGGAAGAAGAAGCGTATGAGGAGATTGAAGAGGAAGAGACGAA 1 2.3255813953488373 No Hit GAGTACGGGGGATCGGAGTAATGATTAACAGGGACAGTCGGGCTGTCTCTT 1 2.3255813953488373 No Hit CAACATACTCAAACTCGCCAATGTAACCGTGCTTTTGCATGACGATAAGGA 1 2.3255813953488373 No Hit CTTAATGACAGACTCAGAAGCCAAGAAAGCATGGTAAGACTTAGCAAGCTT 1 2.3255813953488373 No Hit GTCTTAATCACATCCATTGTTTGAAGAATCAAGTAATCTGATTTCTCCGGA 1 2.3255813953488373 No Hit CTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCAC 1 2.3255813953488373 No Hit GATTGGTTGGCTTCGATGAAGATCGTCAAGCTACGAACATTCTGAAACTTT 1 2.3255813953488373 No Hit CTCTATGAGGGCGGTAAGAGGAAGATAATCGAAAAGGTCTGTAAAATCTGT 1 2.3255813953488373 No Hit CAGTAGGCTAACTAAAGCAGACGATTGGAAGTGTCTGTCTAAGCAGTGAGG 1 2.3255813953488373 No Hit GTGTACATACCTTCGGCGGCGACGAAGAGCTCCTTTTGTTTCTTGAAACGG 1 2.3255813953488373 No Hit GCTCTGAAACTCCCTAGGAAACGTTTTGCTATAGGTGGAAAACTTCTGTTC 1 2.3255813953488373 No Hit GTCTTCCTCTTGTCCTGATCATCAGGAACAGGTTGAAGTATGCTTTGACTT 1 2.3255813953488373 No Hit GTTTTTAATAAACAGTTGCAGCGGCCTGGTATCTGCGACTGCCAATAGCTT 1 2.3255813953488373 No Hit GTATAATTACTGGTTCTTTTAAGATTGACATGAGAAGCATTGATGGGCAGT 1 2.3255813953488373 No Hit CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACCTGTCTCTTATACACA 1 2.3255813953488373 No Hit CTCACCCACACACCCAGGGCTTTCCGGCTGATAACCTCGGTCTATCAGCTG 1 2.3255813953488373 No Hit CATCACTATTCTCCGAGACGAATCTCTCTTCGTGTAAATCGACGATCCTTC 1 2.3255813953488373 No Hit CAAGCATAACAAGGACACAGTTGAGTCTAGCGGAGAAGCTAAACACAGCTG 1 2.3255813953488373 No Hit CACTTGAGGTCTTCATGTGCAGCATTGTCCGTAAAATGGGTTATGGCTGTC 1 2.3255813953488373 No Hit CATTTAACTAAAACCCCTACGCACTTATATAGAGGAGACAAGCTGTCTCTT 1 2.3255813953488373 No Hit CTTTCTACAGAGATTGATATGATCAAGTTAGTTGGACAGTTCTACTTTGCT 1 2.3255813953488373 No Hit GTAACACACTGCGTTTACGGCGCTATCTTTAACGACAATACGTTTATACCC 1 2.3255813953488373 No Hit GTCTTTTATGTCTTCAACGTTTTCACTTAGGATTTTGTTCTTTGGGGTAAG 1 2.3255813953488373 No Hit CTCCGTATCCTCATGAACTACACTTCTCCTTCTCCCCATGATTCTACTAAA 1 2.3255813953488373 No Hit GGGTAGGATGCAAGACCCGCACCATTGGTAATAACCCATCCAAGAGCGAAA 1 2.3255813953488373 No Hit ATGGACTACTCAATGTCGAAGATGTGAAGAAATGGAAAATGATAGATGACT 1 2.3255813953488373 No Hit TCTCATACCTCTGATACTTCTTCACAAAGTGAAGGTAATCCCTTCGCACGA 1 2.3255813953488373 No Hit GAACAATGTTGCTGAAGGATGTGTTGGTCGTGTTGCTGCTAAGCTTGAGAT 1 2.3255813953488373 No Hit ATCGTCGATACTCCCTCAAGTATTGACAATCGGATTAAGCTCATTGAAGAA 1 2.3255813953488373 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 2.3255813953488373 37 0.0 0.0 2.3255813953488373 38 0.0 0.0 4.651162790697675 39 0.0 0.0 6.976744186046512 >>END_MODULE >>Kmer Content pass >>END_MODULE