FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_75.3410000000aaa8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_75.3410000000aaa8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAAGAAGCCATACTCGTTCTAAGATAAGCTACATCGATACTGTCTCTTAT11.3513513513513513No Hit
GGAATGGAAAGGATTACGTGTAACCGTCAAGCTCACAGCTGTCTCTTATAC11.3513513513513513No Hit
ATTGAGGACCCCTTCGACCAAGATGACTGGGAAGCTTGGTCGGTACTGCGT11.3513513513513513No Hit
CGATATGTCCTTCTCCTCTGTTTTGCTGCTTGGTTCACTTGGATGTGAGAA11.3513513513513513No Hit
CAACGATACTCTCAAACTTGAAGTCCAGATCTTGTCCTTCTCTGTCTCTTA11.3513513513513513No Hit
CTTTCGGCTTATACCCCAAAACCGATAATATAACATCTGTCTCTTATACAC11.3513513513513513No Hit
GATTTATGGGAGTCCACCCCATCATCCGTTCCTCAGAATCGGTCTGACGCA11.3513513513513513No Hit
CACTGTCACTGCAGATGGCCAATTCTCCAAGAGGTATTTGAAGTACTTGAC11.3513513513513513No Hit
ATTTGGATACCTAGACATTGCATTAATGGGATACTACAGCTGGTTCAAGGC11.3513513513513513No Hit
TAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGT11.3513513513513513No Hit
TCTGAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCGTCGGCTGTCAGCG11.3513513513513513No Hit
GTGAAGCATCCCAGATGATCAGATATCAGAGTTATAACCATCACAATTCCA11.3513513513513513No Hit
CATCAATGGATGATCAGATGGAGAAAACTAGAGTTGTTGAGTTACAGGGGA11.3513513513513513No Hit
GCAAGATTTAGCCAAAAGGAAATATTCAGCTACAACAGGTGAGGCAAACTG11.3513513513513513No Hit
GTATCAACGCAGAGTACGGGATAACGCTTAATCCTTAAAAACCGTCCAGAT11.3513513513513513No Hit
GAAAATAGAGACAGTGGTCGCAGGGAATTACTTAGAAATGGAGAGGGAAGA11.3513513513513513No Hit
GATAGGGAGAGATTCTTGTTCCAGAGTTATTAGTCTTTGAGTCAGAGATCT11.3513513513513513No Hit
CTCGTAGACAGCGCCTGGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT11.3513513513513513No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC11.3513513513513513No Hit
GGGCTCAAGTGATCTACCGAAGCTATGGATTCAACTCGCGAAGCGAGTTGT11.3513513513513513No Hit
GTAGTACGGATATTAGAGAACCTGCAAGACCAAGCACAGATCAGAGAGATA11.3513513513513513No Hit
ACTTTGGATCTTAGCACCCCAAAAGTCTGTCTGTACAAACGAGAACGGCCT11.3513513513513513No Hit
TCTTTTTATAGTGTGACTGACAACAAACAAACCCCCCGTACTCTGCGTTGA11.3513513513513513No Hit
ATCTCAGGGTCCACTTCAAGAACACTAGAGAAACAGCGCACGCCATAAGGA11.3513513513513513No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCCA11.3513513513513513No Hit
GATAATGTCAGCCCGGATTCTGTTTCACCTTCTAAAGATCTGTCTCTTATA11.3513513513513513No Hit
GTTAGATACATTGGGTGAGGAATCATACAAGGACAGTACACTGATTATGCA11.3513513513513513No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGTACTCTGCG11.3513513513513513No Hit
CGTCCGTTTTAACCCAATCGGAACACCAAACAACCTCGGACTCGCTTCTTC11.3513513513513513No Hit
ATGGAATGTCACCCAATGTATCTTATCGTTTAGCACTACTCCTGAACCCAT11.3513513513513513No Hit
ATTCTAAGAACTCCACCGTCATCTATAAGAAGGTTTGATCCTTTAATACTG11.3513513513513513No Hit
TTGCTATACAGAACCAAAGTGGGTAACATTTTCATCGTGTTCAAGCTGTCT11.3513513513513513No Hit
GTGAAATAGTTCCTGAAACCATGCGCCTACAAGCGGTCGGAGCCTGTCTCT11.3513513513513513No Hit
GTTTTCGACATTGCTTGTCTTTTGAACAAGACTTCTATATTTAAGTTGAGT11.3513513513513513No Hit
TCTGATAACAGCCTCAGGATCATTTCTTCGAATAAGTTCTCTCAGATGTGC11.3513513513513513No Hit
CCATTCGACCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAG11.3513513513513513No Hit
CTTTACACAAACACGTTACAATGTCGTAAATAAACAAACAAACCTGAATCA11.3513513513513513No Hit
GTTGATACCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC11.3513513513513513No Hit
NTTTTACAGTATCTCAACTCATACAAGGGAACTTGTGGTTACCATCAACTG11.3513513513513513No Hit
GGTAAACGATGGAGGAGATATTTGCTCTGATAGTCTCAATGATTCTCATAG11.3513513513513513No Hit
CTTTGCATATTGAATACCCAATGCTGTTTCAACTGAGTAATGTTTCTGTTG11.3513513513513513No Hit
ACCTAGGAAGGTCTTTGTGACTATCTCAATTAATAGAAGACAGCAGAGGAG11.3513513513513513No Hit
GTGAAAGCTTCTGTAATGGTTGTGGTTATCGGTGTTGGGATTTGTACTGTG11.3513513513513513No Hit
CAGGATCACCTCGCGAAGTCGAGGTGAGGTTTGTATGCGCAGAAACAAGGG11.3513513513513513No Hit
ATCCAGTCCCATGTGTTTGAACTGGTATTGTGTGTATCTCTTGACTCTCGT11.3513513513513513No Hit
GTCCACCATCGCCCGAGTTTCCCGACCCATGGCTACCCGAGGGTACGCCGA11.3513513513513513No Hit
GTACGGGACAATTCAGATTCCAATTTTCTCAAACTCTAAAATCAATCTCTC11.3513513513513513No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTT11.3513513513513513No Hit
CTTTAGAACCTTATGGTTGTGTCAAGAAGTCTTGTGTACTTTAGCTGTCTC11.3513513513513513No Hit
CTGTATCAACGCAGAGTACGGGGATCATTCGCACCAAAGAGAAGAACAAAA11.3513513513513513No Hit
CTAGTATGCCCTCCAAAAGTGATGACTTAAATTTGCAGAGTTCATCACAGC11.3513513513513513No Hit
TTTCATCTGTCCCCTCCCCTTTGTTTTTTCTCTTTTAATTCCAAATTACAA11.3513513513513513No Hit
AATCTGGCTCACGCCGATTTGAACTCAGCTCATGTTCAATTTTAATAGTCG11.3513513513513513No Hit
ACAAGCTACATGGGAAACAAGAGATTACTCATAGACCTGTCTCTTATACAC11.3513513513513513No Hit
GGCATTCTCGATGCCGGCCAGGGTCGAGTTGAACAGCCAGCTCTCGCGCGG11.3513513513513513No Hit
CCTTAGCTGATGGTCTGGGTTGTTTCCCTCTCGGCTACGGAAGTTAGCTCT11.3513513513513513No Hit
GAATGTACCAATCGCGAGTAATCCGTGAGGCCAATCGTTAAACTCCTCTCT11.3513513513513513No Hit
TGCTACATCCGTAGCTTCGGCTCTTGTGGGTCTAGGAGATTTGATCATGGA11.3513513513513513No Hit
CATTTCGCCTTACCAACCCCAGGGAAACTCCGAATACAGGCCGTTCTCGTT11.3513513513513513No Hit
CACCAATACTACTACTACTATCGACATACTCTTCGTCATCGCCGTGGTCTT11.3513513513513513No Hit
GCAATACTTTTCGTATAGCGGTGTTGTCACAGAAGAGTGTGATCCATACCT11.3513513513513513No Hit
ATCCAGGAATAAGCTGGCAGCCTTCTTAAAATTTCTGGTGGACATGCAGTA11.3513513513513513No Hit
GTATAAACGCAGAGTACGGGATATCCACTTCCAACAAGATTCCTCAAAAGG11.3513513513513513No Hit
GTATAGATATATCAAACGATCCAATTCGGGTTCAGTTTAGAAACCGTGGAT11.3513513513513513No Hit
ATCAAAGGACAGTGTTAGTTTTATTACAATAGTTTGGTATTTGGTCGCGTG11.3513513513513513No Hit
AGCTATCTCCGCAGCGAGCTCCACACCCGACGGACCTCCACCAACAATCAG11.3513513513513513No Hit
CTTTTCCAAGGTTAAACAACTTGCGGATCTTTGATGCCCTCTTGGGACCTG11.3513513513513513No Hit
GATTTACATAGTCCTTCTGAGATTGTTAAGCAGATTACCTGTCTCTTATAC11.3513513513513513No Hit
TATCCAATCTTCAAGTCCAGCATGAAAACCATGATAGTAACGCAAAGGGTG11.3513513513513513No Hit
TGTCTCGTCTGAGGAAGATTTTTCTGGGAAACCTGGTCAGTCGACAGTTCT11.3513513513513513No Hit
GTCATCGGGCACGACCCCGCGCGCGTTCAAGCGGGGCGTGCGCGCTACCGT11.3513513513513513No Hit
CTTCTTGACACAACCATAAGGTTCTAAAGAGAAAAAAGAAAATAAAATCCA11.3513513513513513No Hit
GAGTACGGGGGACAGTATTGCAAAGGAATACACATCGTCACTTGATGGATT11.3513513513513513No Hit
GCATCAGACACCGACCTATTGGGGGATATGTACTGGAACTGTCTCTTATAC11.3513513513513513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTG50.045.041
TGTGAGA50.045.044
TCCTCTG50.045.014
TTCACTT50.045.034
TTTTGCT50.045.021
TGGTTCA50.045.031
TCCTTCT50.045.08
CGATATG50.045.01
TGTCCTT50.045.06
GATATGT50.045.02
ACTTGGA50.045.037
CTCCTCT50.045.013
TATGTCC50.045.04
TGTTTTG50.045.019
GATGTGA50.045.042
TGCTGCT50.045.024
GTTTTGC50.045.020
GGTTCAC50.045.032
GTTCACT50.045.033
CTTGGTT50.045.029