FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_74.3410000000aa9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_74.3410000000aa9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTGATGAGGGTGTTTAACAGGTTTCTTTGTCTTTGTGCAGAAGAGAGA11.0752688172043012No Hit
GTATAATAACCCTATTCGTTTGTTTATTTGTTGTGTTTTGTACCTTTGATG11.0752688172043012No Hit
CATATATACATATAGAGATCAACAAAGATGGGAAGATCACCTGTCTCTTAT11.0752688172043012No Hit
CCCTAATCTTGAGATGGCATCGGAGAAGAAGCTCTCGAACCCTATGAGGGA11.0752688172043012No Hit
GGCAAGGTCCATGGCTAACCAAGGGGTTGCCTGCGGGACAAGTCATAGAAA11.0752688172043012No Hit
TCTCATCGCTGTGTTATGCGTTTTGGTTCTCCCACTTGCAGCCATGGCGGT11.0752688172043012No Hit
AGACGAAGCTTAGCGCCGGGAACAGCCGCAAGTGGCTCAGGAATACCTCCG11.0752688172043012No Hit
GTATATTACAGACCAGAGAAGAACAGAGTAAAAAACATAAAGAAATGGCCG11.0752688172043012No Hit
CGATTTTTTCTTGATATCAGCAGAAAGTATACAATCTGTCTCTTATACACA11.0752688172043012No Hit
ATCCTGAAGAATCCAGATGACCCAGATATGGTTGATGTCTTCATGGATCAC11.0752688172043012No Hit
ACAATGGCGTAATCACCAGAAGCTCTAGCGAAAACACCACGATCACCAACA11.0752688172043012No Hit
GCTCAGCCTGAAAGTGACGAATATCAGATATATTGCAATGAGTCCAACACC11.0752688172043012No Hit
CTCTCGTACTAGGTTGAATTACTATCGCGACGCGGGCATCAGTCTGTCTCT11.0752688172043012No Hit
GCATACAAGAAGACCAGCTACTCCGGTAGTAAGGACCGGTGGTGAAAATCC11.0752688172043012No Hit
GCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGATGTCTCTTATACAC11.0752688172043012No Hit
GAATAGTGACGATGACGATGAGGTGCTTCTTGCAATGGCTGAGGAGTTAGG11.0752688172043012No Hit
CTCAATAGTTGCATGATTGTCCAAAGATGAATTGATCTAGCTGTCTCTTAT11.0752688172043012No Hit
GGCCAAGCCACGTCTCTTCGGATCCTAATGTAACCTTTTTCTCCCCAAGTT11.0752688172043012No Hit
CTCCAAACCACATTGTCAGAAGCTCGGGACCCTTGACACTGATGAAATTAG11.0752688172043012No Hit
TTTTTGTGTGATGTGTCATTTTCAGATGGCTTCGAGAGCTTGAAGAATGTC11.0752688172043012No Hit
GTGCTTCCCAGCACATTCTCCTAACAAAACTGCTATCACTAACAGGCTCTT11.0752688172043012No Hit
GCGAAATCCTCAGAAAACGCCGCCGTGTCGTCTCTGTAAGCTCGAAAACGA11.0752688172043012No Hit
ACTTAGAGGCGTTCAGTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGG11.0752688172043012No Hit
GACTACGCATTTCTGCCTCGCCTTAGGGGCCGACTCACCCTGCGCCGATGA11.0752688172043012No Hit
CCACATCCTTTTCCACTTAACATATATTTTGGGACCTTAGCTGGTGGTCTG11.0752688172043012No Hit
GTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGAGAGAGCCGTTC11.0752688172043012No Hit
TAGCAGGACAGGTCATGGTACTGGCATGAAACGTAATGGTGGAGGTCGTGG11.0752688172043012No Hit
GTTCTTAAACATGCTTATCAAGCTCGTTAACGCCAAGAACACAATGGAGAT11.0752688172043012No Hit
AAGCTCGATTACGTCTTGGCTTTGACTGTTGAGAACTTTCTTGAGCGTCGT11.0752688172043012No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCT11.0752688172043012No Hit
GCATATAGCTTCGGCAATGTGTATCCCTCATAGACGCTAGCTTCCTGAACA11.0752688172043012No Hit
TGACTACCCTTTGTATTTCTATTTGCTTCTTTTTTTCTTCTTCCGAATATC11.0752688172043012No Hit
GCCAATAGGTTAGAAAGACCACAGACTTTATGATCACAAACTTTGGGACTG11.0752688172043012No Hit
GTTTGATATCCATGAGCGTAATAAGGAGCTCCAGAATCGTAAACGCCAGGT11.0752688172043012No Hit
GCTTGACACCAAGGGTGAAAGCAAGAAGAGCGTGCTCACGGGTCTGACCAT11.0752688172043012No Hit
AAGCTATGCTTGAAGATGTTGCTTTCCCCGCTGAGATTGTTGGAAAGCGTA11.0752688172043012No Hit
GCCGCCGAGCACGAGCGGCTGGTGGCGCAGCAGCGTGCGCTGGCCGCCCCG11.0752688172043012No Hit
CCTCCAAACAGTGTAACAAGATTCTGGAGATAGCGGACAAAGAAATAGGTA11.0752688172043012No Hit
ATTATCATAAGTGTACTGTAGTTGAGCCTAGTGATGGAGTAGCTTTAGCAA11.0752688172043012No Hit
CCACTGCTGCCTCCCGTAGGAGTAAGGGCCGTGTCTCAGTCCCCTTGTGGC11.0752688172043012No Hit
AGGCCAAGCTTAAGGGAGGTTTCTATGTTGACCCTGAAGCTAAGCTCTTGT11.0752688172043012No Hit
GTGTGCTTATATTGTTGAACATTATGGTTACACACATCTGAGTCTGTCTCT11.0752688172043012No Hit
GATGTTACCGTCGATGAAACTTGGTTCTCTGATCCTGAGCTTTGCGAGGCC11.0752688172043012No Hit
CATTTTTTCTCTCTCTTAAAACTTCGAAGCGATTTTTGATTTACAGACTAT11.0752688172043012No Hit
GACAAAGACAATTGAGGAAGATAAGACAAGAATGTAAGATGATTACTCCTG11.0752688172043012No Hit
CTTCAGGAATGTCGAGAAGCTGTCGGAGGGCAAGGTGTGAAAACAGAAAAT11.0752688172043012No Hit
GGCATCGCCATGTGTCACGACGAGATCCTCGCCGTCGGGCATGCGCGCCTT11.0752688172043012No Hit
CCCAAATGTCGGTTCAGCTGTTGGACTGCAATCAATGAAAACCTGTCTCTT11.0752688172043012No Hit
CCCTCAGGGGCACCAGCTGCTGGTTGGGAGTAAAGTTGATAGCCTTTACTC11.0752688172043012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0752688172043012No Hit
GGCTTTCACTGGGTACGCTAAGCAGTATGACAGTGAGGATGGCAAGAAGGG11.0752688172043012No Hit
ATTGAACATAATGGGTGCTGATTTGATGATGGAGCCAACAAGAGATCTATC11.0752688172043012No Hit
AGTTGGAGCACGCCTGTCGGGACCCGAAAGACTGTCTCTTATACACATCTC11.0752688172043012No Hit
GTTCTGAACCCAGCTCACGTACCGCTTTAATGGGCGAACAGCCCAACCCTT11.0752688172043012No Hit
GTTACAAACAATTCAATTACACAGCACAAAATCTGGATTACAAAAGAGTTT11.0752688172043012No Hit
GATATATGTATAGTCTCTTTTTCAAGAGCTCTGCTAAGTCTTTTTGTTCTC11.0752688172043012No Hit
ATATTCCCCGGCCAGTGGATGGTGACGGATCCTTACTGTGGCTGTCTCTTA11.0752688172043012No Hit
CAATAAGCAATACACGCAAAAGTTTAACAAATCCAGAACCTGTCTCTTATA11.0752688172043012No Hit
GTAAAAGGCTTTAACTTTTAAGTAATTCTCTACACCGATGATATTAAGAGT11.0752688172043012No Hit
GACTTGGGGAGACCCAAGTTTGGTGCCCAAGCCACATCACGGACCCAATCA11.0752688172043012No Hit
AGACGAAGGAGTTCGTGTTGACCTTGGATCACACTAACTTCACCGATACCA11.0752688172043012No Hit
GAAAAAGAGTTTCTCAAGACATGGAGATTGATAAAATCGAAAGAAAAGCTG11.0752688172043012No Hit
GAAGAAGGAGCATAAGGTTTACCTTTTGCTACAAGAAAAAACTAGGAAAGG11.0752688172043012No Hit
CTTCAAAAGACTTTTCAACTTTAAGAAGGACAGAGATGGTTACTGGAAGTG11.0752688172043012No Hit
GGTGAAGTCGGAGCTTGACCAGTAACGCCGGCGATGAGGACGATACAGATC11.0752688172043012No Hit
TCTTTATATTGTCCTGTGTTTCGTCGTCGTCCCCTATTAAAATTACCTGCC11.0752688172043012No Hit
TATCATAGTATACTGATGACTTCTTTTTCCCCCCGATTTGTTCTCCATTTA11.0752688172043012No Hit
ATCTGGGACATCATCATCATCGTCCTCTTCTTGGATTGTTGCTGGGACATC11.0752688172043012No Hit
GCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGT11.0752688172043012No Hit
AAGGTACATAGAATGATCGAGTACTGGAACAAGATAACAACTGTCTCTTAT11.0752688172043012No Hit
GCATGAAAATTAGAGGGAAGGAAGCAAGTTAATAATCGCATATCATTCCTT11.0752688172043012No Hit
GGATTTTATTTCTCAAGCATCAACCTACATCTAGTAAAAATGAAGACTGTC11.0752688172043012No Hit
GTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGC11.0752688172043012No Hit
GAACCTGGGTGATCTATCTATGACCAGGATGAAGCTTGGGTGAAACTAAGT11.0752688172043012No Hit
GTTCCAATACCACCAATCTTGTAGACATCCTGAAGTGGGAGACGAAGGGGC11.0752688172043012No Hit
AGCCAGTAGCTTTTCCCTTTCTTGTTAGTCTCATCGATGTGCTTTAGATTA11.0752688172043012No Hit
GTATCAACGCAGAGTACGGGGCCGCTCACGCCCGGTCGTACTCATAACCGC11.0752688172043012No Hit
GATATACCCCATATGTATACCAGAACAAACGGTGAAAACTGTATTCCTGTC11.0752688172043012No Hit
GATGAATCTTTATTCGTGGCGGAGCAAAAGAAAGGATGGCCGAGACTTGAT11.0752688172043012No Hit
ATTTATGGAAACTCAACGAATGATGTAGGGATCTTCGGAGGGCAAAACGGT11.0752688172043012No Hit
ATTATGTATCGCTGTTGAATTTGACTCATTCGAATATCAATAATCTGTCTC11.0752688172043012No Hit
GTTTTGCATTACGTAACAATGATTCTAACACCAACAATTATCAGAAACCCT11.0752688172043012No Hit
GATGCGGAGAAAGCAGCATTTGACGAAGCTGAGAAGAAGAGAGAGGAAGAG11.0752688172043012No Hit
GTATCCGGAATATCGAGTGGGATGCAGTTGAATTACCGTCACAGTTTATGG11.0752688172043012No Hit
CGTTCAACGTGGGAGACGCCAGAGACGGTTGTGAGGGACCAATGATTGTGG11.0752688172043012No Hit
GAGTACGGGGCACAAGGCTGAGAATTATTACACCCATGCAAATGTTAAGAA11.0752688172043012No Hit
TCACTACACTGCGCAATAAAAAAATGGCGAATACCCAACTCAAATCCGATG11.0752688172043012No Hit
GTTGTGGCTTTGGCGGTGGTGGTGGAGGTCTCTTTGGTTTCACCTTATTGA11.0752688172043012No Hit
TCTCCTCCCTTTGGACTTGAAGCTGGCACAGTCTTCGAACTCAAAGAGGAA11.0752688172043012No Hit
GACTTTTGTTATGCCTTAAAGCTGATTCTAGTTCTTATAAGAACCTGTCTC11.0752688172043012No Hit
GCTATACATTGACCAAATAACCTTTTTCTAATTGCATCTGATCACAAGTGC11.0752688172043012No Hit
ATGATAAGGAGATGCCTGCTGCTGTAGTTGATGGACATGATCAAGTCACTG11.0752688172043012No Hit
TATCACCGTTGGATGCCATTTAGATCAGAGATCTGTGAAATCGTTGGATCA11.0752688172043012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATTA50.045.05
TTTTGCA50.045.02
TTGCATT50.045.04
AACAATT50.045.033
AACAATG50.045.015
AAACCCT50.045.045
CAATGAT50.045.017
CCAACAA50.045.031
GAAACCC50.045.044
ATGATTC50.045.019
TGATTCT50.045.020
TTATCAG50.045.038
AATTATC50.045.036
ACACCAA50.045.028
ACGTAAC50.045.011
GTTTTGC50.045.01
CAATTAT50.045.035
ACCAACA50.045.030
CAACAAT50.045.032
ATTACGT50.045.08