FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_73.3410000000aa71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_73.3410000000aa71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCC12.4390243902439024No Hit
TAATACTGCTTCAAATCAACGATTTTTTCCTTAGCTTTCTCGATAACCTCA12.4390243902439024No Hit
GTCCTTTGCCTCCCGTGGAGATCCAGCAAAAATAGAGCATGGGATTAAGGT12.4390243902439024No Hit
CGGGAAGTGTGTAAGAGCGTACGGTGGATGCCTTGGCACCAGGAGCCGATG12.4390243902439024No Hit
ATGTGAATCCCGATATCCTACGTGCCTTCGCTGGAACTGGTATCTCCGTCG12.4390243902439024No Hit
GAGTACGGGGTCGCGTGGTCACGATGGCGTGCCTTTTGAAGAATGAGCCAA12.4390243902439024No Hit
GGTGGAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGCCTGACTTGGGT12.4390243902439024No Hit
GTATGGGCCAGAAGACGGGAACATCAAACACCGAGAAGAATGTCATAAGAA12.4390243902439024No Hit
GATATTCGCTTGTTAAGCGGTCAACCTGTTCTGGTGTTAACCCACTGTAGC12.4390243902439024No Hit
ATTACGCACTCTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTC12.4390243902439024No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAG12.4390243902439024No Hit
GTGTCTTCACTGACAAAGACAAGGCTGCAGCTCACTTGAAGGGTGGTGCCA12.4390243902439024No Hit
GTAGTACAGTTGTCAAGCCGGAAGGAACATGTTGTTCAGGGCTTAAGACTG12.4390243902439024No Hit
ACTGTTAGGAATGCTACTTTGTATGATCCCAAAGTGCCTGCTCGTCTCCTA12.4390243902439024No Hit
GTATCAACGCAGAGTACGGGGAATGCATTGGATGGATGCCCGGGCATTGAG12.4390243902439024No Hit
GGAAAGCTACACCTCAGAAGGTGAGAGTCCTGTAGGCGAACGAGCGGTTGA12.4390243902439024No Hit
CCATCAAGCCACAGACTGACAGGTGGGTGTTCCCAGAGACCAAGGCTGGAA12.4390243902439024No Hit
ACACTAGTAACGCCTACAGCCCTGTGCAAGATGTTGCAGATTCATGCAGAA12.4390243902439024No Hit
ATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAG12.4390243902439024No Hit
GTGTTGATGATCAGGGGATTAACTTCGATACTGGAGCTAGAACTGCTTTAG12.4390243902439024No Hit
GAACCACCCTGGTCAGATTGGTAACGGTTACGCCCCAGTTCTCGATTGCCA12.4390243902439024No Hit
CGTTTCGGGGTTGTAGGACCAGTTTTTAAGATTCAACCCCTCAAGCCGAAG12.4390243902439024No Hit
CAAAAAAGTTGTTTTTCCGGTACCATTACGTCCTACAAGACCGTAATGACG12.4390243902439024No Hit
GGGTACGACATAGCAAGAGCCAAGGAAGTGACCATGGAGCTGTCTCTTATA12.4390243902439024No Hit
AGCTTTGGCTAACAGGGTTTTACCACATCCAGGAGGACCGTAGAAAAGAAC12.4390243902439024No Hit
AGCTGCACCACTTGGTGGACGACAAGATGCACGCGCGCTCTACCGGCCCCT12.4390243902439024No Hit
GGCAATGAGCACCTGAGGTCTTGAAAACGTTGCAAGAACTCTGCATTTTTC12.4390243902439024No Hit
GCTTAGCACCTTGAGCAAAGATACGACCAGCACCAAGAGCATTATGACCAA12.4390243902439024No Hit
TTCCCAAACAACCCGACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCG12.4390243902439024No Hit
TGGTACGACAACGAATGGGGTTACAGTTCCCGTGTGGTCGACTTGATCGTC12.4390243902439024No Hit
GAGCTGGAGCTTGAGCGAGGCATGAGGTAGTGAAGAGAGCAACGATGAAGA12.4390243902439024No Hit
GGCTTAAGTTCAGCGCCGACATCTCCACCGAAGAGCTTTGAGACTTTGCTG12.4390243902439024No Hit
GCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTC12.4390243902439024No Hit
CTTCGCCGGAAAGCAGTTGGAGGATGGCAGAACTCTTGCTGACTACAATAT12.4390243902439024No Hit
CTTATGGCTTTGGCACACTTGAGACTATCTGGTTTTTGTTTGGCATTGAAT12.4390243902439024No Hit
GAAATGGAGATCGGAGTTGGTGAGGAATAGAAAAGCAATCTTGAGCTTAGG12.4390243902439024No Hit
CACACACTCTTCAATCCCCTTTCCGTGCACCAGCTGGGACAGAAACCCTCC12.4390243902439024No Hit
ACCAAGACCTTTGATGAATCTAAGGAAACAATCAACAAGGAAATTGAGGAG12.4390243902439024No Hit
GTACGGGATCGGGGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGAT12.4390243902439024No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGAT12.4390243902439024No Hit
ATGCTACAGAGAATCAGTTTGAATCATTTGCACTTTTTGCTTTTTGTTTGG12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)