FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_68.3410000000aa21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_68.3410000000aa21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCATCAACCTTATATTCTCTCCGCATGCGTTCAACATAGATGTCCAAA18.333333333333332No Hit
GATGAAGAAAGGCTAGATGATGTAGGTTATGATGATGTTGGTGGTGTCAGG18.333333333333332No Hit
ATATGGTACAGTTGTAACCCTCAAGTACTTCAAACACAATTGGACAAATGG18.333333333333332No Hit
GTACAATAATGAGAAATTGTCACATTTGAAAGCAAGCGCTCAATAAACATT18.333333333333332No Hit
ATGCATGAGTGCTTTCTATGACCAGATGTTGATCCTGTCTCTATACACATC18.333333333333332No Hit
CTCCAAAGATGTTAAAAGAGGTGTAGAGATTGGGAGGATGTGGGAGAAACA18.333333333333332No Hit
CTTTAATAGAGCCTGCTTTGATAGTCGTGGAATCTTAAAGAACATATGCTA18.333333333333332No Hit
CATGCATTGGTAAGAGACTCTTTCTCAAGACCCATAGCTTCAGACAAAACC18.333333333333332No Hit
GTTGTAGATCTGCTCACCGACGAGATAAACGCCTGTCTCTTATACACATCT18.333333333333332No Hit
AAGCTATCGAATAGAGTTTCTCGACAACAGGCAGTTAAGTCTTTGGATCTC18.333333333333332No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAATC18.333333333333332No Hit
GTCGACAAGGGGACAAAAGAATCAAAACCCACAATTCTTGTCCTGTCTCTT18.333333333333332No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)