##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_3_63.3410000000a9df.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26 Sequences flagged as poor quality 0 Sequence length 51 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61538461538461 0.0 0.0 0.0 0.0 0.0 2 37.11538461538461 0.0 0.0 0.0 0.0 0.0 3 36.92307692307692 0.0 0.0 0.0 0.0 0.0 4 35.42307692307692 0.0 0.0 0.0 0.0 0.0 5 36.88461538461539 0.0 0.0 0.0 0.0 0.0 6 39.23076923076923 0.0 0.0 0.0 0.0 0.0 7 39.30769230769231 0.0 0.0 0.0 0.0 0.0 8 38.34615384615385 0.0 0.0 0.0 0.0 0.0 9 38.03846153846154 0.0 0.0 0.0 0.0 0.0 10 38.34615384615385 0.0 0.0 0.0 0.0 0.0 11 39.15384615384615 0.0 0.0 0.0 0.0 0.0 12 38.88461538461539 0.0 0.0 0.0 0.0 0.0 13 37.73076923076923 0.0 0.0 0.0 0.0 0.0 14 38.88461538461539 0.0 0.0 0.0 0.0 0.0 15 37.69230769230769 0.0 0.0 0.0 0.0 0.0 16 38.38461538461539 0.0 0.0 0.0 0.0 0.0 17 38.34615384615385 0.0 0.0 0.0 0.0 0.0 18 38.34615384615385 0.0 0.0 0.0 0.0 0.0 19 38.5 0.0 0.0 0.0 0.0 0.0 20 38.34615384615385 0.0 0.0 0.0 0.0 0.0 21 39.26923076923077 0.0 0.0 0.0 0.0 0.0 22 37.61538461538461 0.0 0.0 0.0 0.0 0.0 23 38.46153846153846 0.0 0.0 0.0 0.0 0.0 24 38.88461538461539 0.0 0.0 0.0 0.0 0.0 25 38.42307692307692 0.0 0.0 0.0 0.0 0.0 26 38.73076923076923 0.0 0.0 0.0 0.0 0.0 27 38.23076923076923 0.0 0.0 0.0 0.0 0.0 28 37.88461538461539 0.0 0.0 0.0 0.0 0.0 29 38.46153846153846 0.0 0.0 0.0 0.0 0.0 30 39.11538461538461 0.0 0.0 0.0 0.0 0.0 31 39.38461538461539 0.0 0.0 0.0 0.0 0.0 32 38.92307692307692 0.0 0.0 0.0 0.0 0.0 33 37.76923076923077 0.0 0.0 0.0 0.0 0.0 34 37.15384615384615 0.0 0.0 0.0 0.0 0.0 35 38.19230769230769 0.0 0.0 0.0 0.0 0.0 36 39.11538461538461 0.0 0.0 0.0 0.0 0.0 37 38.88461538461539 0.0 0.0 0.0 0.0 0.0 38 38.19230769230769 0.0 0.0 0.0 0.0 0.0 39 38.57692307692308 0.0 0.0 0.0 0.0 0.0 40 39.03846153846154 0.0 0.0 0.0 0.0 0.0 41 39.30769230769231 0.0 0.0 0.0 0.0 0.0 42 37.69230769230769 0.0 0.0 0.0 0.0 0.0 43 39.03846153846154 0.0 0.0 0.0 0.0 0.0 44 39.15384615384615 0.0 0.0 0.0 0.0 0.0 45 38.84615384615385 0.0 0.0 0.0 0.0 0.0 46 38.5 0.0 0.0 0.0 0.0 0.0 47 39.30769230769231 0.0 0.0 0.0 0.0 0.0 48 38.0 0.0 0.0 0.0 0.0 0.0 49 38.76923076923077 0.0 0.0 0.0 0.0 0.0 50 38.5 0.0 0.0 0.0 0.0 0.0 51 37.5 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2205 1 0.0 2205 2 0.0 2205 3 3.0 2205 4 0.0 2205 5 3.0 2205 6 1.0 2205 7 1.0 2205 8 0.0 2205 9 0.0 2205 10 1.0 2205 11 1.0 2205 12 1.0 2205 13 -1.0 2205 14 -1.0 2205 15 0.0 2205 16 0.0 2205 17 0.0 2205 18 1.0 2205 19 1.0 2205 20 0.0 2205 21 0.0 2205 22 1.0 2205 23 0.0 2205 24 1.0 2205 25 0.0 2205 26 0.0 2205 27 0.0 2205 28 0.0 2205 29 0.0 2205 30 0.0 2205 31 1.0 2205 32 0.0 2205 33 0.0 2205 34 0.0 2205 35 1.0 2205 36 1.0 2205 37 4.0 2205 38 1.0 2205 39 1.0 2205 40 0.0 2205 41 4.0 2205 42 1.0 2205 43 1.0 2205 44 0.0 2205 45 -1.0 2205 46 -1.0 2205 47 1.0 2205 48 0.0 2205 49 -1.0 2205 50 0.0 2205 51 3.0 1216 1 0.0 1216 2 0.0 1216 3 -3.0 1216 4 0.0 1216 5 -3.0 1216 6 -1.0 1216 7 -1.0 1216 8 0.0 1216 9 0.0 1216 10 -1.0 1216 11 -1.0 1216 12 -1.0 1216 13 1.0 1216 14 1.0 1216 15 0.0 1216 16 0.0 1216 17 0.0 1216 18 -1.0 1216 19 -1.0 1216 20 0.0 1216 21 0.0 1216 22 -1.0 1216 23 0.0 1216 24 -1.0 1216 25 0.0 1216 26 0.0 1216 27 0.0 1216 28 0.0 1216 29 0.0 1216 30 0.0 1216 31 -1.0 1216 32 0.0 1216 33 0.0 1216 34 0.0 1216 35 -1.0 1216 36 -1.0 1216 37 -4.0 1216 38 -1.0 1216 39 -1.0 1216 40 0.0 1216 41 -4.0 1216 42 -1.0 1216 43 -1.0 1216 44 0.0 1216 45 1.0 1216 46 1.0 1216 47 -1.0 1216 48 0.0 1216 49 1.0 1216 50 0.0 1216 51 -3.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 2.0 33 0.0 34 0.0 35 1.0 36 0.0 37 1.0 38 1.0 39 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.923076923076923 23.076923076923077 15.384615384615385 34.61538461538461 2 11.538461538461538 34.61538461538461 34.61538461538461 19.230769230769234 3 11.538461538461538 30.76923076923077 38.46153846153847 19.230769230769234 4 7.6923076923076925 15.384615384615385 42.30769230769231 34.61538461538461 5 3.8461538461538463 46.15384615384615 42.30769230769231 7.6923076923076925 6 19.230769230769234 53.84615384615385 15.384615384615385 11.538461538461538 7 19.230769230769234 46.15384615384615 19.230769230769234 15.384615384615385 8 23.076923076923077 38.46153846153847 26.923076923076923 11.538461538461538 9 23.076923076923077 11.538461538461538 26.923076923076923 38.46153846153847 10 19.230769230769234 23.076923076923077 46.15384615384615 11.538461538461538 11 30.76923076923077 19.230769230769234 26.923076923076923 23.076923076923077 12 3.8461538461538463 30.76923076923077 46.15384615384615 19.230769230769234 13 23.076923076923077 30.76923076923077 19.230769230769234 26.923076923076923 14 15.384615384615385 26.923076923076923 30.76923076923077 26.923076923076923 15 19.230769230769234 23.076923076923077 26.923076923076923 30.76923076923077 16 19.230769230769234 34.61538461538461 23.076923076923077 23.076923076923077 17 19.230769230769234 38.46153846153847 23.076923076923077 19.230769230769234 18 23.076923076923077 30.76923076923077 26.923076923076923 19.230769230769234 19 23.076923076923077 23.076923076923077 42.30769230769231 11.538461538461538 20 19.230769230769234 26.923076923076923 34.61538461538461 19.230769230769234 21 26.923076923076923 26.923076923076923 23.076923076923077 23.076923076923077 22 11.538461538461538 26.923076923076923 50.0 11.538461538461538 23 7.6923076923076925 34.61538461538461 34.61538461538461 23.076923076923077 24 23.076923076923077 19.230769230769234 26.923076923076923 30.76923076923077 25 30.76923076923077 34.61538461538461 26.923076923076923 7.6923076923076925 26 30.76923076923077 30.76923076923077 26.923076923076923 11.538461538461538 27 11.538461538461538 46.15384615384615 23.076923076923077 19.230769230769234 28 26.923076923076923 15.384615384615385 34.61538461538461 23.076923076923077 29 23.076923076923077 38.46153846153847 23.076923076923077 15.384615384615385 30 23.076923076923077 23.076923076923077 30.76923076923077 23.076923076923077 31 26.923076923076923 30.76923076923077 34.61538461538461 7.6923076923076925 32 15.384615384615385 26.923076923076923 38.46153846153847 19.230769230769234 33 15.384615384615385 34.61538461538461 26.923076923076923 23.076923076923077 34 3.8461538461538463 53.84615384615385 34.61538461538461 7.6923076923076925 35 38.46153846153847 23.076923076923077 23.076923076923077 15.384615384615385 36 19.230769230769234 23.076923076923077 30.76923076923077 26.923076923076923 37 23.076923076923077 34.61538461538461 23.076923076923077 19.230769230769234 38 26.923076923076923 19.230769230769234 42.30769230769231 11.538461538461538 39 23.076923076923077 15.384615384615385 42.30769230769231 19.230769230769234 40 11.538461538461538 19.230769230769234 46.15384615384615 23.076923076923077 41 19.230769230769234 30.76923076923077 42.30769230769231 7.6923076923076925 42 7.6923076923076925 38.46153846153847 23.076923076923077 30.76923076923077 43 19.230769230769234 23.076923076923077 38.46153846153847 19.230769230769234 44 23.076923076923077 11.538461538461538 38.46153846153847 26.923076923076923 45 7.6923076923076925 19.230769230769234 46.15384615384615 26.923076923076923 46 11.538461538461538 11.538461538461538 42.30769230769231 34.61538461538461 47 30.76923076923077 23.076923076923077 34.61538461538461 11.538461538461538 48 23.076923076923077 34.61538461538461 19.230769230769234 23.076923076923077 49 23.076923076923077 26.923076923076923 34.61538461538461 15.384615384615385 50 30.76923076923077 30.76923076923077 23.076923076923077 15.384615384615385 51 15.384615384615385 38.46153846153847 30.76923076923077 15.384615384615385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 1.0 28 1.0 29 1.0 30 1.0 31 1.0 32 1.5 33 2.0 34 2.0 35 2.0 36 2.0 37 2.0 38 3.5 39 5.0 40 4.0 41 3.0 42 4.5 43 6.0 44 3.5 45 1.0 46 0.5 47 0.0 48 1.0 49 2.0 50 1.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 26.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCACATGTACCATGACATGTACATGCGTGTTAAGGGTATTGCTGTCTCT 1 3.8461538461538463 No Hit TTGCATAACCATGCCAAGATGCTGGTTGTTTAATAAAAGTACCTTCACTGG 1 3.8461538461538463 No Hit CCCTTGTTATCAAGCTGAGTGTCGTGAATGCAAGTTTTGCAAATCTGGGAA 1 3.8461538461538463 No Hit GCTTTGAGTTTACTAAAGTGTCTCTAATGAATGTGGCCTGTCTCTTATACA 1 3.8461538461538463 No Hit AACATACCCAGAGAACAAACATGTGAAGTTTGTAGCTGATGGGTCTGGAGA 1 3.8461538461538463 No Hit CGATAAGGATGCGAATCAGTGGAAGGAGAGAGGAGCTGGTACTGTGAAGTT 1 3.8461538461538463 No Hit CAAAAAAACTTTGAGTTTTGCAAACAAGGTTTCAAACTTGAATAATGTCTG 1 3.8461538461538463 No Hit TCCCATAACTGTTTTTCCAAAACTAAAGCCATTACCTTGTGAAACAGAGAT 1 3.8461538461538463 No Hit GATAAGAGGAACAGCAGCGTTTCCTTGTAGTTTATTCTGTTTAGCCAAAGC 1 3.8461538461538463 No Hit ATGGTACTCAGGACCATTGGTAAGTGAGAATGTAACATACCCTTTCGATGA 1 3.8461538461538463 No Hit GTTCAAAAGACAAGCAATGTCGAAAAACACACTACTGAAAAAGCAACAGAT 1 3.8461538461538463 No Hit ATCTTATTGTCTGTGGGAAACTTTAGAAGTGTCTGTTCTCTTGTGTCCTGT 1 3.8461538461538463 No Hit CGTCTAAGCCTACCAAGTTACAAGAACCACCAACTAAACCTAGAACTTGTT 1 3.8461538461538463 No Hit GTTTTCGTTCTTCTCATCTTATTTTTTTGCTGCTGTATTTGCTGTCTCTTA 1 3.8461538461538463 No Hit CATCAAAGGATCATGCTTCAGTTCAGCTTAACATTGGTCATCTTGATGCTA 1 3.8461538461538463 No Hit AGATTCTTCTATTAACAATCATCAGCTTCAGTATTCGGTCTGTCTCTTATA 1 3.8461538461538463 No Hit GTACAAGATGGTGGTCAAGAGAGAAAAGGGACCAACCTTAGAAGATGGCGG 1 3.8461538461538463 No Hit TTCGTAGCTGCTTTTAAGTCCTTTGGATAAGAGAGTCTTTACTGTTGCTGT 1 3.8461538461538463 No Hit GCTTTTGCCTCAACCGTTTCTTTGTCTTTCTCAACTACTTCACTTCCACAG 1 3.8461538461538463 No Hit TATCTGAAAGGACCATCCTAAAAGACCCCTTTTTGTTGATTACCTCAGACT 1 3.8461538461538463 No Hit CATTAATGGGATCAGAAGATTTCTCTTTCTACCAACAAGCAATCCCGGGAC 1 3.8461538461538463 No Hit CTAACATTTTCTTCTTCTTTTGTCTTTCAAAAGACAGTTTTTTTCTTCACA 1 3.8461538461538463 No Hit GAATGGAATGGAATTGAATGGAATGGGAACGAATGGAGTGTAATTTTATGC 1 3.8461538461538463 No Hit ACATCACACTTTCTGGTGTCACACGACCTGTTCTGAGACTGTCTCTTATAC 1 3.8461538461538463 No Hit CAACAACACTTTACTCCACGATTCGTAAATGAAATAAGCAACAGATTTAAA 1 3.8461538461538463 No Hit CTTTATAATAGCTACCGGCACTCCAGCTAGGTACGGATCTTTCCTGAATAA 1 3.8461538461538463 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 3.8461538461538463 39 0.0 0.0 7.6923076923076925 >>END_MODULE >>Kmer Content pass >>END_MODULE