FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_63.3410000000a9df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_63.3410000000a9df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCACATGTACCATGACATGTACATGCGTGTTAAGGGTATTGCTGTCTCT13.8461538461538463No Hit
TTGCATAACCATGCCAAGATGCTGGTTGTTTAATAAAAGTACCTTCACTGG13.8461538461538463No Hit
CCCTTGTTATCAAGCTGAGTGTCGTGAATGCAAGTTTTGCAAATCTGGGAA13.8461538461538463No Hit
GCTTTGAGTTTACTAAAGTGTCTCTAATGAATGTGGCCTGTCTCTTATACA13.8461538461538463No Hit
AACATACCCAGAGAACAAACATGTGAAGTTTGTAGCTGATGGGTCTGGAGA13.8461538461538463No Hit
CGATAAGGATGCGAATCAGTGGAAGGAGAGAGGAGCTGGTACTGTGAAGTT13.8461538461538463No Hit
CAAAAAAACTTTGAGTTTTGCAAACAAGGTTTCAAACTTGAATAATGTCTG13.8461538461538463No Hit
TCCCATAACTGTTTTTCCAAAACTAAAGCCATTACCTTGTGAAACAGAGAT13.8461538461538463No Hit
GATAAGAGGAACAGCAGCGTTTCCTTGTAGTTTATTCTGTTTAGCCAAAGC13.8461538461538463No Hit
ATGGTACTCAGGACCATTGGTAAGTGAGAATGTAACATACCCTTTCGATGA13.8461538461538463No Hit
GTTCAAAAGACAAGCAATGTCGAAAAACACACTACTGAAAAAGCAACAGAT13.8461538461538463No Hit
ATCTTATTGTCTGTGGGAAACTTTAGAAGTGTCTGTTCTCTTGTGTCCTGT13.8461538461538463No Hit
CGTCTAAGCCTACCAAGTTACAAGAACCACCAACTAAACCTAGAACTTGTT13.8461538461538463No Hit
GTTTTCGTTCTTCTCATCTTATTTTTTTGCTGCTGTATTTGCTGTCTCTTA13.8461538461538463No Hit
CATCAAAGGATCATGCTTCAGTTCAGCTTAACATTGGTCATCTTGATGCTA13.8461538461538463No Hit
AGATTCTTCTATTAACAATCATCAGCTTCAGTATTCGGTCTGTCTCTTATA13.8461538461538463No Hit
GTACAAGATGGTGGTCAAGAGAGAAAAGGGACCAACCTTAGAAGATGGCGG13.8461538461538463No Hit
TTCGTAGCTGCTTTTAAGTCCTTTGGATAAGAGAGTCTTTACTGTTGCTGT13.8461538461538463No Hit
GCTTTTGCCTCAACCGTTTCTTTGTCTTTCTCAACTACTTCACTTCCACAG13.8461538461538463No Hit
TATCTGAAAGGACCATCCTAAAAGACCCCTTTTTGTTGATTACCTCAGACT13.8461538461538463No Hit
CATTAATGGGATCAGAAGATTTCTCTTTCTACCAACAAGCAATCCCGGGAC13.8461538461538463No Hit
CTAACATTTTCTTCTTCTTTTGTCTTTCAAAAGACAGTTTTTTTCTTCACA13.8461538461538463No Hit
GAATGGAATGGAATTGAATGGAATGGGAACGAATGGAGTGTAATTTTATGC13.8461538461538463No Hit
ACATCACACTTTCTGGTGTCACACGACCTGTTCTGAGACTGTCTCTTATAC13.8461538461538463No Hit
CAACAACACTTTACTCCACGATTCGTAAATGAAATAAGCAACAGATTTAAA13.8461538461538463No Hit
CTTTATAATAGCTACCGGCACTCCAGCTAGGTACGGATCTTTCCTGAATAA13.8461538461538463No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)