FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_6.3410000000a5fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_6.3410000000a5fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC42.73972602739726No Hit
GAACAGATGGTTCTTTACCAAGCTCAGGTTCTGAAGAAATTTTCTATTTCG10.684931506849315No Hit
TCGGTAAGCACCGTAAGCATCCCGGAGGTCGTGGTAACGCTGGAGGTATGC10.684931506849315No Hit
CTATGACTCTTCTTGCTTTTGGTTTTTTAATCTTCTTAAGTACTATTTTCA10.684931506849315No Hit
TTCCAAGGCTTTATTGGTCACTCGCGTACACACATTTCTACTCCTGTCTCT10.684931506849315No Hit
GTATCAACGCAGAGTACGGGATCACAACTACTATCACACCAAACTCTGTCT10.684931506849315No Hit
GACTTTCACCAGTAAGTAGAAACTGGTCAAAGTAAAACAAAGCCTGCCAAA10.684931506849315No Hit
CTCGAATTGGAACCTCCCTCGTGGTTTTTCATCGTGTACTCGATGACAGAG10.684931506849315No Hit
GGAATGACCATTCTCTTGACCTTGTCATAAGGAGGTGGTACCTGTCTCTTA10.684931506849315No Hit
CTTTAGAATGTTAGGTCCTTAGATATATTGGGCTGTCATAATACTGTCTCT10.684931506849315No Hit
AGTTGATGCAGAGTTTCAAATCTAAGGAATATGTGAGAGAGACCTTTGCTT10.684931506849315No Hit
GTAACACACCAATTTTCTTCAGCATACTTCCATTCTTCTTCTTTGGTGATG10.684931506849315No Hit
TCTCTTGCCTTCATACATGTTCAAAACAGCTTGCTCATTCGGCGGTTCTCT10.684931506849315No Hit
TGATTCCGTTGTGCGAAGGACTTTAAGAGGTTTTGCAGATCGAAATCGGAA10.684931506849315No Hit
CTGTATGCCCTAAGGGAATGGGTCCTTCTGTGAGGAGGCTTTACGTCCAAG10.684931506849315No Hit
TCACCACAGCGATTAATGATGGTCCAGGGCTGTCTCTTATAAACATCTCCG10.684931506849315No Hit
GAACAACAAGAGAAAATGATTACGGAACTCTCGTTTCTCTGGAGCCTGTCT10.684931506849315No Hit
GGAAAAATATATAGACAATAAGGAGAGGAGAAAAATCTGTCTCTTATACCA10.684931506849315No Hit
TCCCATAATCATCCTCTTAAGACCACCAAGGCCTCTGTTACCCATCACAAG10.684931506849315No Hit
GTGAAGGAGGAGACGGCGTCGCTGAGAAGGCCAATTTGGAGTCTTTAAAAT10.684931506849315No Hit
GTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCT10.684931506849315No Hit
ATCATTTCCTATGGTTTATTGTTTTCTCTACCTGGAACTTGAGTTTACACT10.684931506849315No Hit
GATATGTGTTAGACAAACATAACAATCAAAAATGTCTGTCTCTTATACACA10.684931506849315No Hit
TCTTAGATGTATCAGAAGATCTCGAATTGATTGTTAATTTCCAAAGAGGGA10.684931506849315No Hit
TCTTGTAACTGATGATGATGAAACAAGTCTTGTCTCTGTCTCTTATACACA10.684931506849315No Hit
TTCCTGTACTGCTCTAAGATTTACAGAAGAGAGTATCCAGAGACCAATGAA10.684931506849315No Hit
GTATTTACTGAGTTTCTTTCGGTAATTGTTTTGCATTTTGCATATATTCTG10.684931506849315No Hit
GAATTCTTCTACTTGAATCTTGATGATCCAAATGAAATCATAGAGCTTGTT10.684931506849315No Hit
TATCATTACAGTCTGATTTGAGCTAAGTTCTCTCATCATAAACTCTCCTTG10.684931506849315No Hit
CTATTCATCATGGGTTTGAAGGAGGAATTTGAGGAGCACGCTGAGAAAGTG10.684931506849315No Hit
CGCTTAAACGCTATCAGACGGGTTTCCCCCGACTCTTAGGATCGACTAACC10.684931506849315No Hit
ATGCCATTATGCTTTCTGCTATTTCATTAATGGCAACCCTCTGGGTCAGGT10.684931506849315No Hit
GGCTAGAAGAGCATGAATCCAGTCTAAACAAGGAAGAATTCGATCGTTATT10.684931506849315No Hit
CTATCTTCTTCCGCTGATGTTTTCTACACTATAATAGGTGGCTTTTCTGTG10.684931506849315No Hit
CTGTGAAAGGATCAGGTCATTCTGTTCCTTTGGACCAACCTGTACCTGTCT10.684931506849315No Hit
CCTTGTACCAGTCAAGGTTGGTTGACCTCTCAATCATGTTGTCTCCCTCGA10.684931506849315No Hit
GCGGTAGAGGCTCTCCGACCTCAGATTCTCTGCATACTCTGTCTCCCACCT10.684931506849315No Hit
CCTCACATACAACGCCTCTTGCGCTGCCTTGACACAGTCTCTTATACACAT10.684931506849315No Hit
CTTGGGGCCTATGTTTCTTTGTCATCCACTATCAAATTGCTTGGGAAGTGG10.684931506849315No Hit
GGCAGGAGCTGGGGTTGCAGCAGGTGGTGGCGTGGCTGGGGCAGGGCTGTC10.684931506849315No Hit
GCTCTCTGCTCTAAGTGTAACGGAAAGGGATCAAAATCTGGAGCCTCCTTG10.684931506849315No Hit
CTCTAGAACCGAGTTCCTTGATCAATGCAAAAGTTATAAGAAATCTGTCTC10.684931506849315No Hit
ACTCTGTCTCATTTGTTGTCTCTGGTTTTCTTGTATTCTGTAACCTGTCTC10.684931506849315No Hit
ATTGTGCACCTTCTGTTTTCATCTCCTCTTCCAAACTACTTTATTTTTGCT10.684931506849315No Hit
TAATTTATTTGCTACTTTAAGTACTTTGTGTCTTGTTATGTTTTTGAGTCT10.684931506849315No Hit
CATATATACCAGTGAAATCATACTCAAAACGTTTAGCAGGGTCCATCAGCA10.684931506849315No Hit
CAATTCGTATTCCATATTTCTGGAATGGATTCTCTGACATCAGATTATGAC10.684931506849315No Hit
GTGTGTGAGTGTTATGTGATGCTTTGGTCCCGTACTCTGCGTTGATACCTG10.684931506849315No Hit
ACAAAGCTCTCTTCATACTCCCCATAGATGAACTAAACCCTCTTTTATAAC10.684931506849315No Hit
TACCAACACCTTATAGATACAATTTTCATCAACCCACTTTTGGGTTTTTTC10.684931506849315No Hit
CTTTTATCCCTTAGTGAACCCTGTGTGATTCCTGTCTCTTATACACATCTC10.684931506849315No Hit
TACTGAAACTGAGGAACAAAGGCGTCATTTTGCAACAAACCAATGGAACTA10.684931506849315No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT10.684931506849315No Hit
TCCGTAGATCGTCGATAATCGGACGGTGTACGATGGAAGTAGAAGTTTACG10.684931506849315No Hit
GCGATACATAATAGAGAGATAAAAGACATCAAACATAACCAACATGAAATC10.684931506849315No Hit
TACAGAAGCCAAAACTTGATTTCCAATAGAAAGAAATATGCCATAACACAG10.684931506849315No Hit
AAGTTGTAGTGTTGGCAGAGAGAGGCAAGGGTTAGATTACTGAGAGAGAGA10.684931506849315No Hit
TTTCAAGACAGCAACTGAGGATGATGTCCCATCACCATTCATCATCATATT10.684931506849315No Hit
GTTGACAGGAGTGGGGCTTACATCGTTAGGCTGTCTCTTATACACATCTCC10.684931506849315No Hit
GGCTTAGTGTTACTGTGTGGCACACCAGGAGCAGGTCCTGTCTCTTATACA10.684931506849315No Hit
TCTTGGCTCAGCGTATTGTAAGCAGCTTCTTGCTCCTGTCTCTTATACACA10.684931506849315No Hit
ATATATAAGTCATGAATCATTCACAGTTAATGTATCGACCTGTCTCTTATA10.684931506849315No Hit
CTCCAATGCAAAGGGGATTGTAAGAATCCCTTATTATGGATTAGGGCTGTC10.684931506849315No Hit
CATCAAAGAGGAATATCAGCGAAGAAACAGCATTCCTTTGGACCTGTCTCT10.684931506849315No Hit
CTGGGAACTCGTTCTTAAGCGCCTCGACAAACTTGCTGCGGATTTGATGTT10.684931506849315No Hit
ATTCAATTCCATTCCGCACCATTCCATTCCATTCCATTCCATTCCGTTCCA10.684931506849315No Hit
GTCACATACATACGTCCTCCACCAGATGCAATCTGAGCATCCACACCATTC10.684931506849315No Hit
GACATAACCACTACGGCGATCGAGATTGAGATTTAGATTCTTAATCTCCCC10.684931506849315No Hit
CGCTATGATGCAACAGACTCTGAAACTTTAAGTGAACCCTTGTTAGGAAGT10.684931506849315No Hit
GGCGAAGATGGCGTCGACGCGAAAGCTTCAGATTTCATCAACAAATTCAAA10.684931506849315No Hit
GCTTAAAGAACACAACATTCTTAGATTGCAGACTTCTTACTACTCTTACTA10.684931506849315No Hit
TCTCCATACTCTTCAAGCACCTCTCGACTGCATAATCAGCATAGCTACCAC10.684931506849315No Hit
GGGTGTGGAGAAGCAAGTCATCAGACCCGGCAACGGTCCCAAACCCGCTCC10.684931506849315No Hit
CATTCGACCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGT10.684931506849315No Hit
CCTTTACATCATTTCGGTTAAAGGAATCAAGGGTCGTCTTAACCGTTTGCC10.684931506849315No Hit
CTTCAATCCTTGATGTTTCGAGAAATCTGATTTCCTCGGTGAAAGAATGAC10.684931506849315No Hit
TCTGTGTCCTTCTCGGTAACAGCAACAGTACTGTCTATTATACACATCTCC10.684931506849315No Hit
TCAAAGATCCTTCTTGGACTCTTCTCATCAAGAGTCAAAAGATCTCTAGCA10.684931506849315No Hit
AGTCCATTAATTAAAATAAACACATGATCCAGATCTCTCAAATATTTTCAA10.684931506849315No Hit
ATGATGCTCCTGGAAATGAGAATGGATTTTCTGGAGGATACAGGAGACCTT10.684931506849315No Hit
GAGTACGGGGATTTAAAAGAGATTCTTGAGAGAAAAACTAATTTGACTCGT10.684931506849315No Hit
TCTCAAAACCGTACTCGTCTTCTTCATTTAGAACCGATCTTCGGACTGTCT10.684931506849315No Hit
TAGATAAACTACGGATATACTCGAACTCTATCCATTTACGGTATTGATTTG10.684931506849315No Hit
TATTTGTATCGTCTGGTAGAGACACGATCTTTTGGAGTGATGAAAGCATCT10.684931506849315No Hit
AATCAGAGTGCTGGCAAGACAGAAACAAAGATAAGCAGCTGTCTCTTATAC10.684931506849315No Hit
TGTAAACACTAGGCATACAATGACAAGTGTATTTGTGAGAAGAGTTTAGCC10.684931506849315No Hit
AAATCATCTTATCCTGATTGTACTTACGAGCAAGCATCATCAAGGAAGGTT10.684931506849315No Hit
CTTTTACTTCTATAGTAGCTGATGTTTCTGCAGTTGGGACAGTAGACACTC10.684931506849315No Hit
GCCTCAAGAAGAGACTCGTGGTGCATCTCAACAGACTTAACCTCAGTGGTC10.684931506849315No Hit
TTCCTTCACTTTGTCTCTGCTGATAACCGGACCAGTGGGTGCAGGAGCGAG10.684931506849315No Hit
ATACTCATCAAAGAGACCAAAGAGGTTATAAAACAGTGTTTGGAGATCTTT10.684931506849315No Hit
GGTGAATATATGTATCTTTAGAGGTTTCCCAAATAGGAGAGAATTGTTGTT10.684931506849315No Hit
GAATATATGATGATGTTTTGTTCTTCTCTTTGGTGCCCCCGTACTCTGCGT10.684931506849315No Hit
TCTCTTCTCTTCTGGGCATCAGATAATCATAAAAGTTAGCATCACATAGAA10.684931506849315No Hit
CACAAAGGATGAGGCAAGACAAGTGGACAAGTTAGGGAGATATGCTCATGT10.684931506849315No Hit
ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC10.684931506849315TruSeq Adapter, Index 27 (95% over 22bp)
GTTTATGCGCTGTTGTTGCTTCTTTTTGGCCTCGCGGATGCTACAGTTCAA10.684931506849315No Hit
CAACTACACCGCCTTCAACCATAGTTAGCAAGACTATGAATCCCATATACC10.684931506849315No Hit
GTAGAAAACTTTCATGTGATAATCTGAGTGAGGGATAGCTCAGGATCTGTC10.684931506849315No Hit
CGCTGAGATCACTCCTGCAGCAGAAGCTAGAGAAGGGAGAAGATGAAGAAG10.684931506849315No Hit
AGATGAAGAAGCTCCGGTTGAAACTCCGGTGGTTGTGGAGGATGAGAGCAA10.684931506849315No Hit
CATAACAACAGACACCTGATAGGTCAGAAAGTATTGCAAAGTGCTTCTTCC10.684931506849315No Hit
GTAGAGAATAGAGGCAAATCCCTATTCTTAATATCGAGATGTGATGGGGAG10.684931506849315No Hit
GTGAAAAGACAATGCAACGATTTTTTAGCCATCAAGTATCATCAACTCCTC10.684931506849315No Hit
GTGTTTCTGGTGTAAATTACTTGGGCTCTGTGCTTTGAAACCAGCTTGATA10.684931506849315No Hit
GTTTTACCCTACTGATGACCGCGTCGCGATAGTAATTCAACCTAGTACGAG10.684931506849315No Hit
GCATTTGGAAGATCTTGCTTGTTAGCAAAAACGAGAAGCACAGCATCTCTC10.684931506849315No Hit
GAGCGTGCTCACGGGTCTGACCATCCTTAGAGATACCAGCCTCAAAACCTC10.684931506849315No Hit
TCCTAGCATTGAACCATTCACTGCAGCAGTCTGCTGCTCATCATTCCGATT10.684931506849315No Hit
TCTCTGTTATCACCAAGAGGTTTCTTACAGAGGAAACACGAGTCCAAGAAA10.684931506849315No Hit
CGTGGATGGTGGTTTTATTGGTTAACTTCTACCTGTCTCTTATACACATCT10.684931506849315No Hit
GGATGATATCGCTATCATTCTTTTAAGCCAATATATCGCCAATATGATCCG10.684931506849315No Hit
CATTCATTTGAGCCGACAGTTCCATTCTTTTCTCTCTATCAAACTCTGTTT10.684931506849315No Hit
GGCTTGGTTGGAGTCATCTTCACCATACCAGCATCACCATTCTTCAAGAAC10.684931506849315No Hit
GAACAAAAGGGTAAAAGCTCGTTTGATTCTGATTTTCAGTACGAATACTGT10.684931506849315No Hit
ACACAGTAAAGTGGCAATAAACAGCAGCATCAACAACCTGTCTCTTATACA10.684931506849315No Hit
CTTTTTTCCCCTTTCCCTATGTACTAAGAAAAATATTATTTGTTGGCAAGT10.684931506849315No Hit
ATCCAGAGCAGCTCATTTCTGGGAAAGAAGATGCTGCTAACAACTTCGCTA10.684931506849315No Hit
GTCTCTGCTTCATTCAACTTGTTTTTCCCGGGGAAACATTTTCGTTGCTTC10.684931506849315No Hit
AGAATGGAGAGTCTTGTCCATCACCAGAGTCTTGTCTCTGCAAAGATCTAA10.684931506849315No Hit
ACATATATTCAAATGGCCACCATCGAGGTTGAACAAGTGACTCCAGTAGCA10.684931506849315No Hit
GTCCTAGTACTGCCCCTGCTGTACTCTGTTTTCATGTTCTGAATTCTATTT10.684931506849315No Hit
CTTTCAAGATGCAAAACGAAGAGGGTCAGGTCACTGAGCTTTACATTCCTA10.684931506849315No Hit
GTATCAACGCAGAGTACGGGATTACAAAGCAGAAGAAACACCTGTCTCTTA10.684931506849315No Hit
GTCTTGTAACGGAGAAATCTTTGAAATGGAGATATGAATATTCTTACTTCT10.684931506849315No Hit
ATATACAGCTCTTTATGTTTCTGATCCTTATTGGTACACATCTTACAAAAC10.684931506849315No Hit
ATTACAAATTGCGTTAACAAACATAATCATCTTCCCATGGTTTTTACATAA10.684931506849315No Hit
ATCAACGCAGAGTACGGGGACCCAACAAAGACAAAACGTAACTGTCTTCTT10.684931506849315No Hit
ATCTCATACAAGCCGGTCAAGCACGGTAGGCCTGGTGTTGGTGCTACCCAC10.684931506849315No Hit
TACACACCTCTAATATAGCGCCAGAATAGGTAACCTTGCTCATGGAATCCT10.684931506849315No Hit
CTGTTTCTCTATGTATCTATGTGTATTATCAATTCATCACTGTTTATGAGC10.684931506849315No Hit
GCAATGAACAAGTCTAGATCTGATTGCTTAACATCGAACACCTGTCTCTTA10.684931506849315No Hit
CCTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCTGTCTCTTATA10.684931506849315No Hit
CTTGTAGCCTCAACGTCTAACCATTGCTCTACTTCTGTCTCTTATACACAT10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.684931506849315TruSeq Adapter, Index 12 (95% over 21bp)
AAGTCCTTCTCCTTTGCTTTGCGGCCTGGTTCACTTGGATGTGTGATATGA10.684931506849315No Hit
CTGTCCATTACTCCTTGATTCTCTTCTTCCACTGTTTTTACATGATCTCCT10.684931506849315No Hit
CCTTTTGCATCATCGATTCCATCTCAATCTTCAACTTATCTACATGTACTC10.684931506849315No Hit
CAATTAGTCTCTGGGATTGTTGGTGTTCGTTTAGTGGAGGACTTTGGAAGC10.684931506849315No Hit
GCTCATATCCATCCCCTTCCTTCAAGACTTTCTTCACAACCTGTCTCTTAT10.684931506849315No Hit
ATTAAGGGTAACACAAGTGAGCACAAATAGACTGTCTATGTTATAGCCTGT10.684931506849315No Hit
GTACAAGCACCTCACATTTTGCCAGTTTTTCTTCAAGAGAGAAACGAGGAA10.684931506849315No Hit
GATTGAATTTGTACAAGTCAATCTCTGCGATAACCGGAACGTTAATCGGTT10.684931506849315No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers