FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_51.3410000000a908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_51.3410000000a908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718440
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC47770.6649128667668839No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5050.042.3253645
ATGCCGT5650.037.43241544
CCGAGCC5850.036.53727714
CGAGCCC5850.036.53727715
GCCCACG5900.036.2276418
AGCCCAC5950.035.92320317
TATGCCG5900.035.84629443
CCCACGA6150.035.12080819
CCACGAG6200.034.83757820
CGTATGC6250.034.55887641
CACGAGA6450.033.8361121
GTATGCC6350.033.66032442
TCCGAGC6450.033.1384613
ACGAGAC6550.032.9760322
GAGCCCA6500.032.88354516
TATCTCG6450.032.7896336
CTCGTAT6700.032.2377639
CGAGACT6600.032.04441523
CTCCGAG6700.031.90194912
TCTCGTA6850.031.5318238
Produced by FastQC (version 0.11.2)