FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_50.3410000000a8fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_50.3410000000a8fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968511
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC46630.4814607165019293TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT10980.11336990493654693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4950.041.36197345
ATGCCGT6000.034.49861544
TATGCCG6200.033.02286543
GCCCACG6300.032.49869518
CCCACGA6300.032.49869519
CGTATGC6550.031.60178641
GTATGCC6550.031.25828742
CGAGACT6550.031.25828723
CCACGAG6550.031.25828720
CACGAGA6700.030.55847421
CTCGTAT6700.030.22266639
TCGTATG6850.029.8893140
AGCCCAC7100.028.8368717
ACGAGAC7200.028.43635722
CGAGCCC7250.028.24024415
AGACTAC6950.028.16433725
GACTACT7000.027.64174726
TCTCGTA7400.027.36376838
GGGGCAG507.159213E-526.99891518
CCGAGCC7700.026.5898414
Produced by FastQC (version 0.11.2)