FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_49.3410000000a8d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_49.3410000000a8d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582053
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC8190.14070883579330407RNA PCR Primer, Index 27 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA1550.034.8381119
GCCCACG1550.033.38652418
TATGCCG1600.032.34319743
ATGCCGT1750.029.5709244
CGTATGC1800.028.74950641
GTATGCC1800.028.74950642
CGAGACG1850.026.75629623
CTCGTAT1950.026.53800639
TCGTATG1950.026.53800640
TATCTCG1900.026.05218536
CCGAGCC2000.025.87455614
CGAGCCC2000.025.87455615
ATTGCTA2350.025.85062231
TGCCGTC2100.025.71384445
AGCCCAC2350.024.8931917
GACGAAT2200.024.54503326
CTAAGGC651.5222749E-524.2303524
ACGAATT2050.024.14592627
CACGAGA2200.023.52232421
ACGAGAC2300.023.47785822
Produced by FastQC (version 0.11.2)