FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_39.3410000000a830.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_39.3410000000a830.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367652
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC250.002107643335.99862310
ATACGCG250.002107643335.99862324
TGCCGTC2850.032.36718445
GCCCACG2850.031.57774218
TATGCCG2850.030.78829843
ATGCCGT2900.030.25746344
CCCACGA3100.029.03114919
CCACGAG3200.028.12392420
CCGAGCC3300.027.27168714
CGAGCCC3300.027.27168715
CGTATGC3250.026.99896841
TCCGAGC3400.026.46957813
AGCCCAC3450.026.08595817
GTATGCC3500.025.0704742
CGCTAGT450.001226947124.99904614
TCGTATG3600.024.3740740
TTAGCGT602.468479E-422.4991424
CGTGTGA602.468479E-422.49914210
GCGCAGT500.00226533122.4991434
GAGCCCA4000.022.4991416
Produced by FastQC (version 0.11.2)