FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_38.3410000000a823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_38.3410000000a823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747333
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC14120.18893853208676722TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1900.031.9726418
CGTATGC1900.031.9726441
GCGCGCT651.4026227E-831.1528311
TATGCCG2050.028.53565243
CCGAGCC2150.028.2548914
GGTATCA4450.027.3427261
TCGTATG2300.026.4121840
CTCGTAT2350.025.8502239
TACCCGG801.0530857E-725.3116726
CCCACGA2400.025.31167219
GTATGCC2400.025.31167242
CGCGCGC551.3678812E-424.54465110
CGAGCCC2600.024.22997915
CGTCGGG751.6968042E-623.99921614
GCCAGGC751.6968042E-623.99921627
ATGCCGT2450.023.8767744
GACTACT2350.022.97797426
CGAGACT2750.022.90834223
ACGAGAC2850.022.8939922
GCGTATA500.002245003422.5324331
Produced by FastQC (version 0.11.2)