FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_37.3410000000a809.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_37.3410000000a809.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205929
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC10580.5137693088394544RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.1296563378640211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC250.002103381335.99902727
TATGCCG1300.034.61445243
CGGGGGC401.5546644E-533.7490917
CTCGTAT1350.033.33243639
ATGCCGT1350.033.33243644
CCACGAG1450.032.58532720
GCCCACG1400.032.14198718
CCCACGA1400.032.14198719
TATCTCG1400.032.14198736
TCTCGTA1400.032.14198738
TCGTATG1400.032.14198740
CGAATTG1350.031.66581328
CGTATGC1450.031.03364641
CTCCGAG1450.031.03364612
CACGAGA1450.031.03364621
CCGAGCC1450.031.03364614
TGCCGTC1450.031.03364645
ACGAATT1400.030.5348927
GCGCATA300.00510779730.0356581
CTGCTCG300.005138243529.9991919
Produced by FastQC (version 0.11.2)