FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_3.3410000000a5c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_3.3410000000a5c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518850
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC13530.26076900838392597No Hit
GTACTTAAGAAGATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAA7710.14859786065336802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.12257877999421798No Hit
ACATAATAGAGAGATAAAAGACATCAAACATAACCAACATGAAATCAACTA5920.11409848703864316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA1600.037.966819
GCCCACG1650.035.45272418
CGAGCCC1650.035.45272415
CGAGACT1650.035.45272423
CGTATGC1650.034.08915741
CCACGAG1800.032.49833320
TATGCCG1750.032.14430643
CTCGTAT1800.031.24839639
AGCCCAC1900.030.7878917
GTATGCC1850.030.40384342
GACTAGA1900.029.60373926
TCCGAGC2000.029.24849513
TATCTCG1850.029.18768936
AGACTAG1950.028.8446725
TCGTATG1950.028.8446740
GAGACTA2100.027.85571124
GAGCCCA2200.027.61221516
TCCTATC2050.027.43761333
CCGAGCC2150.027.20790314
ACGAGAC2150.027.20790322
Produced by FastQC (version 0.11.2)