FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_27.3410000000a767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_27.3410000000a767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612707
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC29930.18558857870648543No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTCG250.002107616735.99910745
TGCCGTC3550.033.59071445
TACCCGC352.821289E-432.1420635
TATGCCG4300.027.73186943
GCCCACG4350.027.41311318
CCGAGCC4400.027.101614
CGAGCCC4450.026.79708915
ATGCCGT4450.026.79708944
CGTATGC4800.024.84313641
GAGCCCA5050.024.0588116
CCCACGA5000.023.84940719
CCACGAG5100.023.82293920
AGCCCAC5050.023.61327617
GTATGCC5200.022.93212342
TCCGAGC5450.022.70585813
CCGCGAG500.002265329722.4994419
ACCCGCT500.002265329722.4994416
CCCGCTG500.002265329722.4994417
TACCGCG500.002265329722.4994417
CTCCGAG5450.022.29302412
Produced by FastQC (version 0.11.2)