FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_26.3410000000a75b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_26.3410000000a75b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862265
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC22250.2580413214035128TruSeq Adapter, Index 12 (95% over 21bp)
GTACTTAAGAAGATTAAAAAACCAAAAGCAAGAAGAGTCATAGACTCAAAA10840.1257154123152395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3100.039.92007444
TATGCCG3300.037.50067543
CGTATGC3350.036.93882441
GCCCACG3400.036.39560718
GTATGCC3400.036.39560742
GCGCGTC250.002107230535.9985613
CGAGCCC3550.034.8577615
CCCACGA3600.034.37362719
CCGAGCC3850.033.31035614
TCTCGTA3850.032.1415738
CGAGACT3850.031.5571823
TGCCGTC3950.030.7600545
CTCGTAT4100.030.1817239
CAGGCGT300.00514755829.99880236
AGCCCAC4150.029.81808717
CCACGAG4200.029.46310820
AGACTAC4000.029.2488325
TCCGAGC4250.029.11648613
TCGTATG4350.028.44713840
GACTACT4250.027.52831326
Produced by FastQC (version 0.11.2)