FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_25.3410000000a731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_25.3410000000a731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453442
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC8460.18657292443134954RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGC301.1390292E-437.49889811
GACCGGG300.005144750229.9991177
AGGCCAC300.005144750229.99911710
CGTATGC901.1874363E-824.99926441
GTATTAA651.5059253E-524.2621651
GTATCAA7650.022.97091
GACAGTA500.002262897822.4993387
TATGCCG1003.6332494E-822.49933843
CGTAATC500.002262897822.4993383
GTATGCC1056.089249E-821.42794242
TATAACG654.2336178E-420.7686214
GTCCCAT550.003903527320.4810471
TAGGTCG550.00393358820.4539455
GCTATCT1109.953874E-820.45394534
TATACAG1009.032665E-720.2494055
GGTATCA3350.020.175361
GCCCACG1151.5905243E-719.56464418
CCCACGA1151.5905243E-719.56464419
TATCTCG1151.5905243E-719.56464436
CGAGACG1151.5905243E-719.56464423
Produced by FastQC (version 0.11.2)