FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_24.3410000000a724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_24.3410000000a724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC42.9197080291970803TruSeq Adapter, Index 12 (95% over 21bp)
CTTCTATAGGGAACCCTATCTTCATCGAGATTTCTCATGTCCTCAAACCTA10.7299270072992701No Hit
GAATAGGACAAATGAAGAAGTTCTACATGCGACTATATCGTAAGTTCATAA10.7299270072992701No Hit
GTAAGCGTAGTTCTCGAGAGCGTTCTTGGCTTCAACCTTCTTCTTGTGCTC10.7299270072992701No Hit
GTATCTGCGCAAGGCCTTGGTTGCATGGGCCTCTCCGCTTTCTACGGTGCT10.7299270072992701No Hit
GCAAGATACTCTCCACTACTCTGCCAGTACATCTTGCAGTCGCTCACACTG10.7299270072992701No Hit
GCTTTACTCGGTCGTATCCCGTCTGCTGTGGGATACCAGCCAACTCTGGCT10.7299270072992701No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTAAACACCCCC10.7299270072992701No Hit
CTCGTGTGCTATCCATTCTCACGTTGTGAGCTGTCTCTTATACACATCTCC10.7299270072992701No Hit
CTGCTTACCTCATACAAACAAGTCAAAATCGGCAATCCTCTTGAGAAAGGG10.7299270072992701No Hit
GCAGAAGGCTTCTCAAAACCATAAGCATAAATACCTGTCTCTTATACACAT10.7299270072992701No Hit
CCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCC10.7299270072992701No Hit
CTCCTTGGTCCGTGTTACAAGACGGGTCGAATGGGGAGCCCACAGGCCGAC10.7299270072992701No Hit
GACAAGAAGAATTCAATTTTCATTGTCAGTGGAAGAGGGAGGGAAAGTTTA10.7299270072992701No Hit
GAAGAGCAGTGAACATTCATATGTTCTGTGGTCGTAGACCGGGTCTGTTGC10.7299270072992701No Hit
TTCTAGAAGCTGTCACTGAAGAGCAAATGGCAATAAGGAAAGATAAAAAAG10.7299270072992701No Hit
CTCTGTGAGAGAAGCTGATCAGAAAGGTTTACCTGATTCAATTAGTGTTTA10.7299270072992701No Hit
CGTAGAACTGCCATGAGAGATCAAAGGAGCGAGAGTAGAAGAATGGAAGAT10.7299270072992701No Hit
CTTACCGGGTGGTCCCGGCAGATTCACAGCAGATTCCACGAGCCCGCTGCT10.7299270072992701No Hit
CTCTCAAGTCTCCGTCCGCCACTTCACATTCTCATGCGCCGGTGAGAAAGC10.7299270072992701No Hit
ACGGTGGACATGGCTAATCCAGAGGAGCTTAAGAAAGTTTTCGACCAGTTT10.7299270072992701No Hit
CGCTAAACCTCGAGGTCTCGGTTCCTCTCTCGGGTTACTTGATCGTCGTCC10.7299270072992701No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTCGCCCCGAAAATG10.7299270072992701No Hit
ATGTAATATCAGTCGATAAAAGTACTCAGGTTACATTATTCATCCAGGCAA10.7299270072992701No Hit
GGGTTGCAGCCTGCTCGTCGGTCAGTTCCCATTTGCCAAACAGATTGAGGA10.7299270072992701No Hit
GTATCAACGCAGAGTACGGGTCCATTGAAACATCAGCAGAAAGACATGAGG10.7299270072992701No Hit
GAGAAGCAGCATCAAGGGTAGAAGTCTGTTGGCATCTTTTCACTGACAGTA10.7299270072992701No Hit
GTCTCACGACGTTCTAAACCCAACTAACGTACCACTTGAATCGGCGAACAA10.7299270072992701No Hit
TGGATAGCGTCTGCAAGGTGAGGTACAGTCTTCGAACTCAGACCAGCCATG10.7299270072992701No Hit
CATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAA10.7299270072992701No Hit
CCATGGTGTTTGGTATGCTAACCAAACCACTGATTAGATACCTAATGCCAC10.7299270072992701No Hit
GCTCCCATCGATATGTGAAGGAAGCGATTTATAAACCCAGGAGCTCAGATA10.7299270072992701No Hit
AGTTGGCACAATGGCGAATCCCAAGTATTCTTCCTCACACTTCTTTTGGCA10.7299270072992701No Hit
GCTCTGTTTTCCATTTTTTTTTTTTTGGTTTCATTGTTACTGAAAATCATT10.7299270072992701No Hit
GTGGAAGAATGTATTAAACCTAAACTCGGTGGAGAAAAAATGGTAGTTTTT10.7299270072992701No Hit
CTTCGACGTGCTGGGCATCACCAAGCATGCAAATCTTCATCTTTGGCCTAG10.7299270072992701No Hit
GAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGT10.7299270072992701No Hit
GTACCGAAGAAGAAGTCTAAGAAGTGAAGAGGATATATAGTGTGAAGCTAT10.7299270072992701No Hit
CGTTTTATGCGGAAGAGAAGTTACTTAAATCTGAGGCAGTGGCGATGCGTG10.7299270072992701No Hit
TAGTAAGACTTAGCCTTGGGGTTAATCCCGGTACTGATGATAACAGCAATG10.7299270072992701No Hit
GATAAATATTGGATAGATACAAACGCGCATAGGATCAGCCGAAGAAAGAAA10.7299270072992701No Hit
AACTATACATATGCACATTCTGGATTCAGTAATGGAGACAGATAGAAGAAG10.7299270072992701No Hit
GTGAAGATGTATTCGGGTCAGAACAAGATCCACAAGGATAAGGGTGTTGCA10.7299270072992701No Hit
GAGTATTATTGAGGCCATGAAGACGGACAGCGCTTTTAGCCTGCCTATAGG10.7299270072992701No Hit
CCCGTGAAGCTAGGTTGCCAACAAAGGTACTGTGGATGCGTAGGATGCGTG10.7299270072992701No Hit
GCCTTCCACCACATAGCCAGCCACCTCAGCGGCTGTCTCTTATACACATCT10.7299270072992701No Hit
GCCATACATTCAAGTCAAGATCAAGGATGGTGAGACTAAGGTTTTCAGTCC10.7299270072992701No Hit
CCATCAAGCCACAGACTGACAGGTGGGTGTTCCCAGAGACCAAGGCTGGAA10.7299270072992701No Hit
ATCCTGAAGAGCGACCCAGTTTTTGATAGCCCCGAGATAGTTGCCTAAGTG10.7299270072992701No Hit
GATCTTCCCTCGTCCAACTTCTGGACCTCTCCGCCCTGTTGTGCATGGTCA10.7299270072992701No Hit
GATTGAGAGTGTTCCAAATCTGCAAGTTATCAAGTTGATGCAGAGTTTCAA10.7299270072992701No Hit
TCTTTCTTCTTAGCCGAGTACTTCGGTAGATTAGTTGGAACGATTGATGAT10.7299270072992701No Hit
GCTAGAGGCTATGTGGAGGCTAGGACAAAGTGGATTAATCTCAAGAACGGT10.7299270072992701No Hit
ATCACAAACAAGAGCTCTTTGTTGCTTCAATTGAATCCGGTTCATAAGATG10.7299270072992701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT10.7299270072992701No Hit
ATACACAAGTTATGGAGCAAAATAAAAGAGTGTTTGTTCTGTCTCTTATAC10.7299270072992701No Hit
GAGATCTGTTTCTCCTTCGCCTCAGATGGCTGTTCCCGATGTGTTTAAGGA10.7299270072992701No Hit
ATCTTCCTCTTTAGAGCTCGAAACCCTGCTGCTGATATACCCGAGTATTTT10.7299270072992701No Hit
CAATTAGGGTCTGCATCCATATCTCTAGTAACCTGAATCTTAGCTCCAGAC10.7299270072992701No Hit
CCATGAGGCCTAGTGACTTTGCCCCAAATGCAACGGTAGTGAGAACCGTTC10.7299270072992701No Hit
CAAAGAGGCTGAGAACTGTGACTGCCTTCAAGGATTTCAGGTGTGCCATTC10.7299270072992701No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATG10.7299270072992701No Hit
GTTCAAAGACGTGAAGATCTACTTTGTTTCCCCTGAAATTGTGAAAATGAA10.7299270072992701No Hit
CTTCAGTGGTGTTTACACCTCCACAGACTCCAAACACAGCAACTGGCTTAT10.7299270072992701No Hit
TACATACTCTTCCATGAATACGGTATCTGCCATTTCCCACCTTCTTCCCCA10.7299270072992701No Hit
TTCTCACTCAGATCTAAGAAAGCTGACCACAATCAGTGATGGTAACGACTT10.7299270072992701No Hit
GTTCAACTGACACTCTTCCATTCGCCACAATGGGTTCGGGTTCTCTTGCTG10.7299270072992701No Hit
ATCTAGTGATGACCACCGGGAAGCTTCTCTTTAATCTTATCCATCGTACTT10.7299270072992701No Hit
ATGCCACGGTGGAGGCCGGAGATGAAAAGAAAGAGTCACCGATTTTTCGAC10.7299270072992701No Hit
TCACTAAGATTATTGTTTGCATTAAAGTACCACCAATCATCCCAGTCCATA10.7299270072992701No Hit
TTGTATCAGTATAATCAAAGCAATAAACTCTGTCTCTTATACACATCTCCG10.7299270072992701No Hit
TGATGAGATAGATTTTGAGTTTCTTGGTAACATTAGAGAAAAAGAATGGAG10.7299270072992701No Hit
GGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGCCCCCGCCGACGTCTCC10.7299270072992701No Hit
GGTTAGTAGAGAGACACAGTGGTTTTGTTCCTTGTGGCATGTCTTCTCGAA10.7299270072992701No Hit
CTCACAAAATCACCAGCCAAGTATTCGTTCTTAGAAAGCTGAGCTTCATAG10.7299270072992701No Hit
GAATTACTTCCAAGGTAGCACCAACAATGGACCTTGAGTATGGCTTCTTGG10.7299270072992701No Hit
GTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTCATTTCT10.7299270072992701No Hit
CCCTATATCCTTGTGGTTTGTGGAGAACAGCCATCGAACCAGATTTTTCAT10.7299270072992701No Hit
GCTCAATCCTCCTCGTTCTGCTCCCCAGACTCCACACCTGTCTCTTATACA10.7299270072992701No Hit
GAGCACAGAGTCGTGGTAGATGGAAATGTCATAACAAGCAGAGCTCCAGGG10.7299270072992701No Hit
GTCCAATGTTTAACATGCTTGTTGGACTTGGATCGGGCCTTGGTGATGCAA10.7299270072992701No Hit
TCTCTGAACCGGAGTTAGCTGCGGAGCACGAGGAAGTGAAGGAGAACAAGA10.7299270072992701No Hit
GTATCAACGCAGAGTACGGGTGCGACCCCAGGTCAGGCGGGGTTACCCGCT10.7299270072992701No Hit
ATCTGGGAGTTTAAGAGAGTTCTTGTTCTTCAAACCCTTTGCTACCTCAGT10.7299270072992701No Hit
ATGTAGAAGAGAAAACAAACAACAAACAAACAAACAAAAAACTTAAATACA10.7299270072992701No Hit
CAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAA10.7299270072992701No Hit
GTCTTATGCAATGTTATTTTGATGCTTAAGATAAAGTTACTGCAGATGAGG10.7299270072992701No Hit
GCCGAAAGGCGATGCTGAAACTCGGAATGAAACCTGTTACTGATGTTAGCA10.7299270072992701No Hit
CAATGGTACTGAGCATACCAAGAGCAGCAACAGCAACACCATACATCTGTC10.7299270072992701No Hit
CCTATTTCCTTTCTTAGTTCTTGACCCTGTTCTGGGAAGATTGTGCAAGCA10.7299270072992701No Hit
CCTTGTCAGTTTATTCATCATCACCCACACACTTGATGCTCGTGTTAAGGT10.7299270072992701No Hit
CCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCA10.7299270072992701No Hit
GTACTAATGAAGAGATTGTTCAGTTTGCTTGTACTCGTTCTGTCTCTTATA10.7299270072992701No Hit
GTACATGACCAGAATCTGCCCAAGAGACACAGATATGAGAGTTTTGTGGCA10.7299270072992701No Hit
GTATTGTTGTGCTTCATCAGGCTGATATTGGAGAGCTGTCTCTTATACACA10.7299270072992701No Hit
GATATAAACCAAACAAGCTTTGATTTCACACCTAGAAGTCTCGACAGATAT10.7299270072992701No Hit
TAATTACATTGATGCCACCGTGGATAACGCGTTTGGAGGAGGAGGGAGTAG10.7299270072992701No Hit
ATCAAGGCCAACTTCAATGACAAGTACGAGGAGGACAGGAAGAAATGGGGA10.7299270072992701No Hit
CTCCAGCAAAGGGGACTCAAATATGTATCCCAACGGAATCCAAGAAAGATA10.7299270072992701No Hit
GCTTCAGGGGAGCTCATGCTCCATCCACCCCACATACCAGTAGATGGGTAT10.7299270072992701No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT10.7299270072992701No Hit
ATGCTTAACAGAGAGATATTGAAGGAGACTGATGAATACAAGAGAGCACAA10.7299270072992701No Hit
TCTTAATATAGTGACAATACAATTGGATTCGTAGCTTGTTTTTGAAAACAT10.7299270072992701No Hit
CTCCAGGTTCATGTGATTCCAATCACCACCAAACTTGTGCATTTTAAGAAG10.7299270072992701No Hit
ATTCTAAGGAGAATGCTGAACAGATAATGATTAATAGCTGTCTCTTATACA10.7299270072992701No Hit
GAGGGTTCTTCTTCTCTTCTTCCTAGACGATCTCTCAATCAAGAGTCTAAT10.7299270072992701No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC10.7299270072992701RNA PCR Primer, Index 27 (95% over 21bp)
ATAAAAGTTATTCCACAAACGCATTCGATAAAACATTCAAAACCTTCTTCA10.7299270072992701No Hit
GGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGATCCCACCTCT10.7299270072992701No Hit
ACCATGAACAGATGCACAAGCTGCCTCCCCAGCAGCCATTAGCCCAGGAAT10.7299270072992701No Hit
TTATGATTCTAGGCGAGGGATCTGGAACTAGGAGATGGAGATGTTTAACGA10.7299270072992701No Hit
GGTCAACCCTGTGGGAGCAAAGGTAACAACCATACCAGGCTTGATCATACC10.7299270072992701No Hit
CTCCACGGCCGCTCGTTTTCACACACACGAGGTTGGTCCTAATCAGTGTTG10.7299270072992701No Hit
GAAGTATACTTGTGTAAGATAACAGATAACAGGAAAAAAGCAAAACCCAAA10.7299270072992701No Hit
CGGATACCGTGTTCGAAGTACTTTTGAACATTGACAAACATCAAAGATACG10.7299270072992701No Hit
CCTGTATTGGTATTGCTGGGGCACAGGCGGGTGAAGAGATTACTGTTCTCT10.7299270072992701No Hit
CGCCCCGAATCGATCGGCGGACCGGATTGCTCCGTTCCGCATCCGACCAGG10.7299270072992701No Hit
CCTCCAAAACTTTCACCAAGTACAATGCCGGGATCAGTGTGACAAAACCAG10.7299270072992701No Hit
CTCCGGGCGTCGGCCTGTGGGCTCCCCATTCGACCCGTCTTGAAACACGGA10.7299270072992701No Hit
CATCAAAGCTTGAAGGGCACAGATGGGATCAATCTCAGTCACAATGACTCT10.7299270072992701No Hit
ACATGGTACTGAAAGCATAAACAGGAATGATGTCATTATTGTCTAGATCAT10.7299270072992701No Hit
CTCCGGATTTTCAAGGGCCGCCGGGGGCGCACCGGACACCACGCGACGTGC10.7299270072992701No Hit
CGTTTGTCTTTACCAACCTTCAAAAGCTCAGTGATTCTCTTCTCATAGGGA10.7299270072992701No Hit
GTAGTACAGTGAGTAAAAGATCGGCGTAACAAACTTGGTAGCCATAGCCAA10.7299270072992701No Hit
GTCCCTAAGCAATCACTTAGTGGAAAAGGAAGTGATCGAGCGATGACAACC10.7299270072992701No Hit
GAGTACGGGAATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGC10.7299270072992701No Hit
CCATAATGTGAAGCAAGTTTATGCATGGCATGCTTTAGCTGGAATGGAACA10.7299270072992701No Hit
GTCGGTGAGTCTGTGCGCGGCGGAGAGAGTGTGAATTTGTTGTTCCGATTT10.7299270072992701No Hit
ATCCTACACAAACAAAACCGAACACCAATGCCAAGCTATAGTAAAGGTCCC10.7299270072992701No Hit
CCCACAAAGATGATGATGACTTCAAGTCTCCCATTTGCCAATGGCTACACT10.7299270072992701No Hit
AGTGAGAAGATGGTACTCCATCAATTCAAACACAACCCAAGCAGCTGTAGC10.7299270072992701No Hit
ATGATCATCTTTGTGTTTTTTTTTCCTTCTGTATTACCATTTTGGGCCTTT10.7299270072992701No Hit
GAACTCGTCTTTCGTCATCACAAACTCTTCGAGGTTTACATCTTCCGAGAG10.7299270072992701No Hit
GTGCAGGTAACCAGACCACCAACTCGATAGGGTCAACATCGTGTGCAATGA10.7299270072992701No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers