FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_2.3410000000a5b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_2.3410000000a5b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533063
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC13230.24818830044478793TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCCG250.002106506835.99857345
GCCCACG2050.030.73049218
CCGAGCC2150.030.34763514
GACTACT2350.027.76485826
CGAGCCC2400.027.18642215
CGTATGC2350.026.8074541
TCCGAGC2600.025.96051213
TATGCCG2450.025.71326643
TCGTATG2650.025.47068840
CTCGTAT2500.025.19900139
ATGCCGT2500.025.19900144
TGCCGTC2500.025.19900145
AGACTAC2600.025.09516125
CCCACGA2550.024.70490519
GTATGCC2550.024.70490542
ACTACTT2650.024.62166427
AGCCCAC2600.024.2298117
CGAGACT2950.022.1177723
CTCCGAG2950.021.35508712
TCTCGTA3100.021.04755238
Produced by FastQC (version 0.11.2)