FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_14.3410000000a682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_14.3410000000a682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828079
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC18380.22195949903330478TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2650.029.71606441
GCCCACG2650.028.86703318
TATGCCG2700.028.3324643
ATGCCGT2850.027.63072644
AGCCCAC3000.025.49921217
CCCACGA3050.025.08119419
CGTCGGG1001.3442332E-924.74923714
CTCGTAT3150.024.28496639
CCGAGCC3150.024.28496614
TCGTATG3150.024.28496640
CGAGACT3300.023.86289823
CGCGCGC851.8928768E-723.82279610
GTATGCC3300.023.181142
GGTATCA4400.022.5305961
CTCCGAG3400.022.49930812
TCGGGCG1257.512426E-1021.59933537
CACGAGA3550.021.5486321
TGCCGTC3800.021.31513245
ACTACTT3700.021.28312727
ATTATAC1401.364242E-1020.8922123
Produced by FastQC (version 0.11.2)