FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_13.3410000000a668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_13.3410000000a668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319386
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC4310.1349464284596069RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGG207.0265913E-444.99874531
GCCCACG453.4836987E-529.99916618
GGGTCCG300.00514245829.99916629
AGCCCGC300.00514245829.99916629
GACGGGG300.00514245829.99916641
GCCCGCA300.00514245829.99916630
GGTAGGG300.00514245829.99916623
GTATCAA4200.029.4865471
TAGACAG702.8732757E-828.9277675
TACACAG651.5196061E-524.2300935
GGTATCA1353.6379788E-1223.3509641
GCATTAT702.6794169E-522.5171
GGGGCTC500.002259365322.502944
TACGGGC500.002261422622.49937415
CGGGGCA500.002261422622.49937417
TATCAAC5350.022.28912
TCAACGC5300.021.6503414
CAACGCA5450.021.054465
AACGCAG5500.020.8630546
TATGCCG654.2253284E-420.77190443
Produced by FastQC (version 0.11.2)