FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_12.3410000000a65c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_12.3410000000a65c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC64.411764705882353No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21.4705882352941175TruSeq Adapter, Index 12 (95% over 21bp)
TTCTAAAAGTTTATCCTTTATGTTGTTAAAATCAAAATTCTCTATCAGAAA10.7352941176470588No Hit
CATCAGCTTAACATCTTCCTTGTATTTGTGATACCCATCGGATGTTATATC10.7352941176470588No Hit
AATATATTCTTCTTGATTCATTTAAGTTCCTGATAGATTCTGGTTATTCTG10.7352941176470588No Hit
GTATCTCCAGTCATCTTCATAGTTTTCTCGCTCATTATACGTATTAGATCC10.7352941176470588No Hit
GAAATATATCCAGGAAGATGACAAACGTCACAATTCAACCCGTGGACGATG10.7352941176470588No Hit
CGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAG10.7352941176470588No Hit
ATTTAAAATTCACCGGACCAATCACCGACCGAAACAAAAAATTAAAGTTCA10.7352941176470588No Hit
AGGTATCCACAGCTTCGGTAAATTGTTTTAGCCCCGGTACATTTTCGGCGC10.7352941176470588No Hit
GCTTCAACGCCTCAGGGTGTGGAGAATGTATTCAGCATAGAGGTCCATTGA10.7352941176470588No Hit
TGATAAGAGTGAATAATAATGATGTGGGAGTGGGACTTATGTTGTTTTTTG10.7352941176470588No Hit
GAACTAGAGGAACACAAGGAGAGGATAGGCTCAGCTCTCATGAGATTGTGG10.7352941176470588No Hit
CAATAATAGCATCAAGTCTCAATAACTGATTCATCAACATCTCTATCAGAT10.7352941176470588No Hit
TGTCTGAAGAATCCAAGTGACTCTGATCCACATCATTGGGCCAACCATAGT10.7352941176470588No Hit
CTCTTATGATTCCATAGACCAAGTATATGGTTCCTGTCTCTTATACACATC10.7352941176470588No Hit
CCGTTAACCGTCGTATTCCACCGCCGTTAGATTCTCCGTTAACGGCGTCTA10.7352941176470588No Hit
CAAGGGAATCGTCAAAGATTTCCATATGCAAGATGAAGATGCAGGCAGACC10.7352941176470588No Hit
ATCACGAATAGAGAAATATTGATAAACCAAATGTTATTGCTGTCTCTTATA10.7352941176470588No Hit
GTCCAATTCCAGGAACCTCCAAGATCCAAAACCTCAACCAAAACATTGGAG10.7352941176470588No Hit
GTCTAAGAGTGGAATCATGTGGCCATGAGCCATGAAGGGGAAGAACAAAAT10.7352941176470588No Hit
CATATGAACATACAAGAAGCCTACAGAACTCCAAATAGACTGGACCAGAAA10.7352941176470588No Hit
AATGAGTACAGCCACTTTCGTAGAGATTATTCTTGCTATCATCTTGCCTCC10.7352941176470588No Hit
GCCGTACATCACCACTGGTGCTGCCGGGCCTCTCCACCTTGACGAGAAGCT10.7352941176470588No Hit
GAATCTGGGCTAAACTTTTCAAGTCTTCCCCAGGGTCAATTATTTCCCTTC10.7352941176470588No Hit
TTAGTGAACCATCGAACAATAAAACTGAGTCATTTAGTTGTCTTTGGAACT10.7352941176470588No Hit
GCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGAT10.7352941176470588No Hit
CTGTTAACACTCCGCCATCCAAAATCAGAAAATGGAACATGCTTCCTTTTT10.7352941176470588No Hit
TCCACCGGGTTATTACCAGAGCTGGCATATTGGAGTTCGAGCCAAGACTTC10.7352941176470588No Hit
GTATGGAAATGTAAAATTGAAGTTTTCACTTGGGGAACTCTACATTATTTA10.7352941176470588No Hit
CTTATACACATCTACGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.7352941176470588TruSeq Adapter, Index 12 (95% over 21bp)
AATATCAACCTGTTATCCATCGCCTACGCCTGTCGGCCTCAGCTTAGGACC10.7352941176470588No Hit
AAAAAAAGACTGTTTTTCGTTTGCAAGATAATTTACGAAACTTGTAATATT10.7352941176470588No Hit
AGATCGTATATTAAACTAGTTAAGTCCCGCCTATCTAGATGGTTGTAATAC10.7352941176470588No Hit
TTGCTATTCACAGTGACGCTTGGTTACTGTCTGTCTCGTTTTACTTTGGCT10.7352941176470588No Hit
GTGCGATACTAGGAGGAAACCCGGTCGTACAAGCGCAGAACAACGGATGGG10.7352941176470588No Hit
TCGTCACTTTCGTGTACCCATCGGACGGCAGCCCTTTCGGGGGTTCCTTAG10.7352941176470588No Hit
GAGGAGAATGAGAGATTATTGGATTTGTTCACAGAAGAGCCACCGTGTATA10.7352941176470588No Hit
GATGATTGTTAATCGGATTTGTTCCAACAAGTCAGGATCTTCCACTTTCCG10.7352941176470588No Hit
GAGTACGGGGGACAAAAGCTGACTCTACGCTCTCTCAAAAGTCTGTCTCTT10.7352941176470588No Hit
GGCATTGGAGGACCGAACCCACGTATGTGGCAAAATACGGGGATGACTTGT10.7352941176470588No Hit
GACTTAGCGATTCTTGTGGAGATACAATAAGCCGTAGACATGACTGTGATC10.7352941176470588No Hit
CTTCTGAGCATCCGCCGCTTCCGTCTACAGAATGGATTCTCAAATCAAACA10.7352941176470588No Hit
GTCCACGCGGTTAGACTCGGAAACTTGGGCACCATCTCTCAGAACGTGAGG10.7352941176470588No Hit
GTAATGAATATGATCTTCATAATTCCTAGTAGCTTGTGGCTGTCTCTTATA10.7352941176470588No Hit
ACAATATGTATGTCGGTTCAACAAACAAGTAGTTCCAAGGTTACTGTCTCT10.7352941176470588No Hit
ATAATAAACACCATCATCTTCATCTTCATTGCCTCCATTAATTCCTGTCTC10.7352941176470588No Hit
TAACACGACATCATATTATTTATCCACTTCTAGAATTCCAGTGATAATGAA10.7352941176470588No Hit
TCGTAGGAGCTCCCGCTTGTGGTGATGTGATGAAGCTTCAAATCAAGGTCG10.7352941176470588No Hit
ATTTTAGGGTCACTCCCATGAAGCTCGCGGCTGTCAAAGATACGTTGATAA10.7352941176470588No Hit
GCTCCATCAGACTGGTGGCAGCGGTGGTGGTGCTACGGCCTGACACTTCGT10.7352941176470588No Hit
TTCCTATAGTCATGGCGTTTTCTCAAGGTTGAAGTCCAATCGGAGCCTACT10.7352941176470588No Hit
ATCATACATCCAACGATCCTTTTGTTACAATTAGAGGAAACCAAACGCTTC10.7352941176470588No Hit
ATCCTTGTTAGTTTCTTTTCTTCCGCTTATTGATATGCTTAAACTCAGCGG10.7352941176470588No Hit
CATTGATTGTTTCTTCATGTTCTGCCATGGCTACTTCTTCTCTGGATATCA10.7352941176470588No Hit
GAACCTTCCTCACCTCGTTTGCCAGCTCTATAACATAGTCCTCCTCATGCC10.7352941176470588No Hit
TTGCTAAGCTGTAGCAGCTGGAGAGGGCTGGGAGGTAGAAATTATTGAGTA10.7352941176470588No Hit
GATTGGTAACGGTTACGCCCCAGTTCTCGATTGCCACACCTCTCACATTGC10.7352941176470588No Hit
GAATGACACCGATTCCACCTTTCTCAAGATCCTCGACTTCGTCCTTGATGG10.7352941176470588No Hit
GGTAGGAGCTGGAGTTGGCTCGTTAACTGGAACAGGCGGTGAAGCAATTGG10.7352941176470588No Hit
GTCTACGAGTCGGGTTGTTTTGGGAATGCAGCCCCAATCGGGCGGTAAATT10.7352941176470588No Hit
CCATTGAACCCACAAAGAAGTAGACGAGAGCATCCTCCAAATGAGTTTTAA10.7352941176470588No Hit
GTCCGGGCCTATGTCGCTCCAATATACCGAAATCGCTGTCTCTTATACACA10.7352941176470588No Hit
GTGAGAGAAGCCGTGACGGATTCAGCGGTGCTTGTAACGGATTCTTTGGCT10.7352941176470588No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATCTGTCTC10.7352941176470588No Hit
GCCATAGACCTCGATGGGGACACCGATATCATCTCCAAGGGCTCGCATTTT10.7352941176470588No Hit
GATTTGGGATACTGCTGGACAAGAGAGATTCCAGAGTCTTGGTGCTGCTTT10.7352941176470588No Hit
CCGCTGTCCAAGAGCTCGAAGGTAAGCTCCAGAAAGTCTTGGACTGTCTCT10.7352941176470588No Hit
AGGTGATGGGGGATAAACTCCAAGCTAATCAAACAGAGAGGACTGGATACG10.7352941176470588No Hit
TGGAAGGACTTTGTTCGTGTAGCGAAGAGAATTCGAATCTTGCCGGTCAAG10.7352941176470588No Hit
CAACATAACCATACAAACATTGCATGATCAAGAACTAAACACTGTCTCTTA10.7352941176470588No Hit
CCCCGGAAATCACGCCTTTCCTCGATGCCGCGCAGCGCAAGCTGCTGACCG10.7352941176470588No Hit
GAATACTTCTGGCTCCTTTGAAGCTCAAGTTGACGCACACGACTGTCATAT10.7352941176470588No Hit
GGCATGAAGTGACCAACTGTTTCAGACTCTTCTCCAACTGTTTCAGACTGT10.7352941176470588No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTTAACCGG10.7352941176470588No Hit
ATGTAAAGTATAGTGTGAAATGTGTGTGTGTGGGGGGATTAAGTCCAAGTT10.7352941176470588No Hit
TTGCAACCAATTATTACCCTCATTCCCTTGCAACGTACAAGACTACAACAG10.7352941176470588No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT10.7352941176470588No Hit
CCCACCATCTTCACAGTTCCAGAGAATCCCGGAATCTGGAATCGGCAAATT10.7352941176470588No Hit
CATATAGTCTCTTCGTGCTGTCTCAAGAACCACCTAGAGATAACCCAATGG10.7352941176470588No Hit
CGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGC10.7352941176470588No Hit
ATCAGACATGAATTAGGCACACCTCATTCATTATTTGATGGAAAAACAGGT10.7352941176470588No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC10.7352941176470588No Hit
CTCAGAGACTTTCAAATAGGTCAAACTCTCTCTACTCCAAACCTGTCTCTT10.7352941176470588No Hit
CTTCACAGCACAGTCCAGGTATCCAGCTGCTTTTAAGAAGATATCGATTGA10.7352941176470588No Hit
ATCCTGACATCTCCAAGGTAGCTGGTTCTTACGCGGTTTGTCTTATACCGG10.7352941176470588No Hit
CTCCAAGCATCAGTCCTCTTCCCCGGACATCCCCGATAATCTCGTGTTTTT10.7352941176470588No Hit
CTTGGTATCTCAGATCCTAAGTATTTATAGAAATCATCAGTAAAAGCTGTC10.7352941176470588No Hit
TAACTAATGTGACCCTTGGTGTGACAAGGAGCTGTCTCTTATACACATCTC10.7352941176470588No Hit
TCTGTACACTTTAAAACGAAATCAAGAACTCAAATCAGACTTAAAGGAACA10.7352941176470588No Hit
TCTCAGCGACGGTGACGAGGGAGAAGAACTCTTCCTTGGCATCTTCATCAT10.7352941176470588No Hit
GGAAAGTACATGGCTTGCTGTTTGATGTACCGTGGTGATGTAGTCCCCAAG10.7352941176470588No Hit
AAATAGTACTTAAGAAGATTAAAAAACCAAAAGCAAGAAGAGTCATAGCTG10.7352941176470588No Hit
TATTAAAAATGTCTAGGTGTGGAAGAAGTGGTGATGGATATGAACTGTCTC10.7352941176470588No Hit
CTACCATCCCGGTACCAGTGTCGGAGAGTGTGGTGGAGCATGACCATCCCG10.7352941176470588No Hit
ACTCCGAGTTTGACGAGCTTCAGCCGGGCCATACCCGAGCCATCGCCATCC10.7352941176470588No Hit
GACTTATGTTGTTTTTTGTTTTTTGTTGATCATTGTCTCTTTTATTTTGTC10.7352941176470588No Hit
GTTCATAATGCTTTGTCGAATGAAGCTTTCTGATAGCTTGCCAATTGTCAA10.7352941176470588No Hit
TTTCATCGGTACGCTCAATACATGAATTTCTCACATCCTTTGATCAAAGAG10.7352941176470588No Hit
ATTCAAGATGCACTACAATGAAGAAAGTGAAAGGTCAAGACTTTTAATTGA10.7352941176470588No Hit
GAATAACATTGTTTCTCCTTATGTTTGTAAACAAAGATACAGAGAGATTAA10.7352941176470588No Hit
AGTGAGAACCGTTCTTCTTTGTCTTTGCCTTGTAGATGTAAGCCAAACGCT10.7352941176470588No Hit
CTTCAGGGATTGTTCCTACTCTTCAAAATATTGTCTCAACAGTGAACTTAG10.7352941176470588No Hit
GTAGAAAAAGAGCTTGGACTGTTGTTCCCTAGTAGGATCAGCAACCAGACA10.7352941176470588No Hit
CCCTCGTCCTTTGTACTCCAGAACTTCTGGGATAGATCACCATCTTTCCCG10.7352941176470588No Hit
CCTTCATCGGCTCAGCATGCCAAGGCATCCACCATGCGCCCTTAATAACCC10.7352941176470588No Hit
CTTCCGCTTAGAGACATCATCTCCTGATGCTTATATACCTGTCTCTTATAC10.7352941176470588No Hit
GCATTAATCCATCCGCAAACCTCGTCGAGAAGCGCACAATCTTGTCCAACA10.7352941176470588No Hit
CAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGGTAACAAC10.7352941176470588No Hit
GGCTTTGACCAAGCTGCTGAGACTATGATTAAAAAAGTGAAGCAAGCATTG10.7352941176470588No Hit
GTCTTTTATCATATTTTCAAACCAAACCCAAGAAGATTTTCCGTACAATTT10.7352941176470588No Hit
TACTAGTAGTAATCACTTCTGTCTCTCTTTTCTGTGTCTCTACTAAAGACA10.7352941176470588No Hit
GTCAAGGTGTGCTGAGGAGTTTAAACTCAGAAAGAAGCAGAACGATGTGCT10.7352941176470588No Hit
GGCAAGGACTTAGCCACGCTCTCCCTCTGCAAACACCTGTCTCTTATACAC10.7352941176470588No Hit
GCCGCAGCCTGCCAAGGGCGTCCCCGCCCCGGTGCTGGGAAGCCCCGGCCG10.7352941176470588No Hit
CATATCTAGCGTTGGAACAGCTGAAGATCGGTCCAAGTTTCCGCACGACGG10.7352941176470588No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT10.7352941176470588No Hit
GTTCTCTTCTGATCAAGAACTCTTAACCTATGTCACAGATGCTGTCTCTTA10.7352941176470588No Hit
CTATATGGCAGAGAGAGTGAGATCTTCTTAAGAAAACAAATCATGTTTAGT10.7352941176470588No Hit
GTACATTGACGAGGTCTGGTCTCACAAGAACCCTATCCTTCCTTCTGATCC10.7352941176470588No Hit
CTGTTATTGTTCTAGCTGGTGAAATGCTTCACGTTGCAGAAGCGTTTCTTG10.7352941176470588No Hit
CGAATATCGTGCAGAACAATCTCAGATCTTCTTTTCAATACATAGCTCCTC10.7352941176470588No Hit
GTATCAACGCAGAGTACGGGGAGTCAGAGAAGAGAGAAAAAAGTTCCATTT10.7352941176470588No Hit
GTAATACTGATCGTACAGATCATCGTACCTCAGACCGTATCTGCGAAGGCG10.7352941176470588No Hit
CCTTGGAAGCAGCAACTTCCAAGAGTTGCTTGACCTTAGTGATATAGTCAT10.7352941176470588No Hit
CTATTCTCACTCTTGCCCCGCGGATATATTGGGTGGCGATTGTCTCGAATC10.7352941176470588No Hit
GAAGAAGAAATTAAGACCTGTCTCTTTCTTTCACCATCTCTCGTACGTAGG10.7352941176470588No Hit
AGATTGTTCTTGACGGTAACGACATCGAGCTTGTTTCAAGGCTGTCTCTTA10.7352941176470588No Hit
AGGATGACCCGAGCACAAATGCTGGTAGAGGCTCTAATCTGACAGAAGATG10.7352941176470588No Hit
GAGTACGGGAGATAATCTTGCATTAACAAATGATGAATTTGATAAGATTAT10.7352941176470588No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers