FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_3_1.3410000000a590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_3_1.3410000000a590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC74.2682926829268295No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC53.048780487804878TruSeq Adapter, Index 12 (95% over 21bp)
GACCAAAGACAAAGACAAAGACCAAACTGATAAAATGAAGCTGTTCAGGTT10.6097560975609756No Hit
TTCCAGAGCTTCTTTCAATGGGGTAACGAACCGCTGGAACTCCATCTCTTC10.6097560975609756No Hit
ATCCAGACCTTCATAGAGCCCTTCACCGCTAGTTGCGCATGTGCTTTGGAT10.6097560975609756No Hit
ATCCTAAATACCACAAAACATAAACAAAAGCTCTCTTTCACTATCCTGATG10.6097560975609756No Hit
GTCTTGAAGCTCTTGAAAATCCGGTTAATACCGATCGAAATCTCCGGTTTA10.6097560975609756No Hit
ATGTCGAGACATATTTCTCCTGTCTTGAAATGAACATTAGGATGGAATATC10.6097560975609756No Hit
ATATTAAGCTCTACGAATGTTACCATTAGAGCTGTCTCTTAACACATCTCC10.6097560975609756No Hit
CCTTCTGAGGGTGTAGTTTTTCCGTCTTGTACGGGTGTATTTTTGTCAGTG10.6097560975609756No Hit
CTTATCCACATTATTCTATCAACTAGAGGCTGTTAACCTAGGAGACTGTCT10.6097560975609756No Hit
TTCTTTCTCCTCCTCCTCCTCTTACGAAGAAGAGCAAGTCGCCATGAAAGA10.6097560975609756No Hit
GAATGGCGTAACGATGGCCACACTGTCTCCTCCCGAGACTCAGCGAAGTTG10.6097560975609756No Hit
CGGATCAGCTCACCGACGATCAGATCTCTGAGTTTAAGGAAGCTTTTCAGC10.6097560975609756No Hit
CCATGACACTCGGACTGTTGTGCTTTATTCATTAACCAATGAGTAATTTAT10.6097560975609756No Hit
TATTGGTGCTATTACATCCAGGATTTTGGCGCGGATATTTTAAAAAGAAAG10.6097560975609756No Hit
NGTCACCAACTTCCTTAACCTTCCGGCACTGGGCAGGCGTCAGCCCCCCAT10.6097560975609756No Hit
GTGTAAGCACCACCTGCCTTGACCTTGCCACAATCCTTGCAACCCCAGATA10.6097560975609756No Hit
GTATACAATAATGCAAATACAACAATGAGAATACAATCTGTCTCTTATACA10.6097560975609756No Hit
CTGTTGGATTGTATCCTCTTCCAATCGGCCGGACATCAAAACTTGGTCCGA10.6097560975609756No Hit
CTCCAGTAGCAGCTGAGAACATCGAGGTGCCACCACCAAAGGCTGTGGAGT10.6097560975609756No Hit
ATGACAATCTTGGTGTGAAATTTTCTGGTTGTTAACATTAACATACACCTC10.6097560975609756No Hit
CCCTGGAGACATAGTTATTACAGAAGACCAAGCCAATGCCACCAACGAAGA10.6097560975609756No Hit
GCATAGTTTCGGGTATAAGGTTGTTCGGCACCATGAGTGCACCTGTCTCTT10.6097560975609756No Hit
GAACATCAAGAATGTGTTTGCGCAGTAAGAGTTGAAACTCATGAACACATG10.6097560975609756No Hit
CTCCTTAGTTGTTGCCTCTCCGCATCCATTGCTTTAAGTGCTTCATTCGCC10.6097560975609756No Hit
GCCCTATGTCACTTACGGATTCCCTAACTTGAAGAGTGTCAAGGAATTGAT10.6097560975609756No Hit
CTATGACTCCCGCAAAACGTGCTACCGCATTTGCACTTAAACCCCATTACA10.6097560975609756No Hit
ATACTCAGATACGGTAGGAGTGGTTGATCTTGGAGGGGGGTCTGTTCAAAT10.6097560975609756No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC10.6097560975609756TruSeq Adapter, Index 12 (95% over 24bp)
GTTGATGCTCGTAACCAGCGTCTCAAGCGTGCCATGGACCTCTCCATGAAC10.6097560975609756No Hit
TTTTTACTTTTGTTGCTGTGTTTCAATTAAAAGTCTTGACCTTTCACTTTC10.6097560975609756No Hit
TGGTATATGTGTGCGTAAACTCGGAATAGTCGTTTAATGATTGTCTTCACC10.6097560975609756No Hit
CTGTAATATATCCTGAATTTATGGGTAATTATAACTAATTTTAAACTTTCT10.6097560975609756No Hit
GAAGATATAGGAACCAATGGGACTAGCCACGGATATGGTATTGAAGATGGT10.6097560975609756No Hit
CTATATGACCGACCTTTTTCGATCTCATATCGTTTTCTGTTTATTTTATTT10.6097560975609756No Hit
TAATAACACATCTTATGTGGTTTGGGATCGTTATTGGAGATTTGAATGTGA10.6097560975609756No Hit
GACCACGAACATTGTTATTCCCCTTGGTAACCATGATGCTTCTGGTGACTT10.6097560975609756No Hit
CCCATCATCTGATCCAGAGAAGAGGACTCTCGGGTCTACAGGGGAGAACAC10.6097560975609756No Hit
ATGACGGGAGTGCAATAGAGAACCTGAGATCCGATGCGAACAATCAGTCGA10.6097560975609756No Hit
TTCTAAGCCAACCTCCTGGTTGTCTTCGCGACTTCACATCCTTTTCCACTT10.6097560975609756No Hit
CCCATACACATTCTTGAATGTGTCCGAGAGCATATAGTCAGTCACCTCAGG10.6097560975609756No Hit
ATGTTTTGGTGGTGCAATCAGCGACTTCAAACTCGACTGAGCATTTGAGTC10.6097560975609756No Hit
CTATAAACCCGAAAAAGCCTCGAGTTGATGAAACCCAAACCATGTCAATAG10.6097560975609756No Hit
GTTTCAAATTCACAAGAAAAATCACTTGAGTTTCATACAAGAGAGAGAGAC10.6097560975609756No Hit
GTATTCCTCCTTGCTCGTTTCCTGTCCACAAAACTCGTAAATCACGGCGTC10.6097560975609756No Hit
GTCTTGCTTCGATCATAGCTAAAGAGTTGCTCAATGGACAGAGTGTTGTTG10.6097560975609756No Hit
GTGGAGAGCTCTGACACCATTGACAACGTCAAGGCCAAGATCCAAGATAAG10.6097560975609756No Hit
AGAGAATGAAGAAAGATGAGTCAAAGTGTCTTTTTTTATCTCAAAAGAGTT10.6097560975609756No Hit
GTACAAAGTGGTCATGAAGGGATTCAGTACGTCAAGTTTGATTATGTTAAG10.6097560975609756No Hit
CTTTCACATTGTAACTTAAAAACATAACTCAATCTGCAAAACCATCTGATC10.6097560975609756No Hit
ATCAGGAACCCTGAAGGTATCGACATCAACGCTCTCATAAAACACAAGGAC10.6097560975609756No Hit
GACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGG10.6097560975609756No Hit
GAGGTACAGGATCATCCAACAAGGTTGGTCCTTGAGGTGTCTCATCACTTG10.6097560975609756No Hit
GTCTAGATAACAACAAAACTTGATCATGTTCCAAACAAATCTCAAGAGCTG10.6097560975609756No Hit
CCTCTCCGTTGATCGTTCTATCTCTTTCTCAGAGCTGATGAAGAAACTCTA10.6097560975609756No Hit
GCATAGAGAGCATCTCCTTTCTCAGGATGATCAAACTCAGACTGAGGCAAC10.6097560975609756No Hit
CCCGTAGATGACAGTCTATGAAGTTACATCTCAAGCAAAGCCCGTACTCTG10.6097560975609756No Hit
GTTCCTTTGTTTTTCCTGGGGAGCAGATTCAAATCCTTGCATGCTTATCTG10.6097560975609756No Hit
CCACTTACCTCAGAACCTTCAAGGTCGGCGATTACGTCGATGTCAAGGTTA10.6097560975609756No Hit
CTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCG10.6097560975609756No Hit
GAACAAGTAGTAGCAAGATCCCAAGAACCACCAGTTTTATCTTCATGTTCT10.6097560975609756No Hit
GTTTTTCAGTCTGAGCTTCTTGTTGTGAGGAAAAAAAATAGACGTTACTGC10.6097560975609756No Hit
ATTGAAGATCGAACAATAAAAGAGAAGGCACTACGATGTACCGAGTGAGAA10.6097560975609756No Hit
GCCCCGGACAACCATCGCCGGGGACGAGCTACCTCACTGTGTCCCTCCGCA10.6097560975609756No Hit
GTCAACAAACTGAATAGTTCGCTTGGTCTTGATGGTGCCAACCGCTGCGTT10.6097560975609756No Hit
GATCCTTCTCTATCGTGACAGGAGAAATGCTGGATGTTGGGTGTTCATTTT10.6097560975609756No Hit
TCCAAACACTTCTCCAATCACTTTCATTATGCTTTTCCTACAATCTGATCC10.6097560975609756No Hit
CAATCAAAGAAAATGGAAAATGCAAAAAGATCCTAACTCAAAATATCCAGG10.6097560975609756No Hit
TGGTGATGGTGTTACTCATGTGGTTCCGGTGGTTGACGGCTACTCGTTCCC10.6097560975609756No Hit
CCACATCTCCAGGAAATCGAGGTTACGGATCGTGGGCTGTCTCTTATACAC10.6097560975609756No Hit
GTGTGCAATATGTTACCATCAAAATGCCAACTAGTAAGTGTTGTTGCAAAA10.6097560975609756No Hit
GTTTTGTCTCTCTCCAATTTATCTTTCCAGTTGCTTTGAGAACATTATAAG10.6097560975609756No Hit
TTTTTATACCTTCAAGATCCTCAAATGTTGACTTCTTCCTGATAGAAGGCA10.6097560975609756No Hit
TCTCTGTATTTCTTCAACAGACGCCTAAGAACACGCATCCTACGCATCCAC10.6097560975609756No Hit
GATTTGGGGAAAGCTCTAGAAGGCGCTGACCTCGTCATTATTCCAGCTGGT10.6097560975609756No Hit
GTTTAACTAACACTCTCTCGCCCTCTCTCTCTCTCTCCCTCTCTCAAATTC10.6097560975609756No Hit
ATCCTTAGCGATACGGAGGACAGTACCGCCGGCGAAGCAACCTTGCTGGTA10.6097560975609756No Hit
GTACAACATGGTGAACTTGCGAGACATAGGCAAGAATCTCAGTTCCTGCAA10.6097560975609756No Hit
CTCCCAGCACCTTGTCGACGACCTTGGCTTGCTCGCTGGGGGAGAGTGCGG10.6097560975609756No Hit
ACAGAAATGGGTCTTGATGTTTTCAAAAGCCGCACCAACTAAGTAAAACAA10.6097560975609756No Hit
CAAGATGATCTATGATTTGGTACTCCTTTACTGGGAGAGAATGAAGATAGA10.6097560975609756No Hit
ACTTATGACCGATCTTTTCGTCAACGAATCCAGCAAAGGTTGGGTTACCGA10.6097560975609756No Hit
TCGTCAACACTGTGGAGATCAGAGGCATGCTCAGCCTCTTCGCGACCTTTC10.6097560975609756No Hit
TCCCCAATGCTCGTCGCGCCGTTACTCCCGCCACAGCCACCACCCCCGTAC10.6097560975609756No Hit
GTGTGTGGTTTAACTTTATTGAGAATATGTGTTTTGTCTGTCTCTTATAAC10.6097560975609756No Hit
CATCTGATAAAGCCTCTTAACACCAGTAGTAGTCTCCTCAGAGACACCAAC10.6097560975609756No Hit
GTCACGCTGTTCCTTCAACCTCGAGGCGAGAAGCTTCTGGTAATCAGCAGC10.6097560975609756No Hit
GAGAAGCACCCTTCTGCCATGTATTGTGATTTGATTGTTGTTGTGCATCAA10.6097560975609756No Hit
TCTCCACATGGGACCTTTTGAGGGTTTCTCAGGCGTTGAAGGGATAGTTGC10.6097560975609756No Hit
CTGGTGTTGAGATTCATCCGATTGACGAGGCGGAAGACTGTCTCTTATACA10.6097560975609756No Hit
CTACCGGCAAACTCGTCAGCCTCAGCGAACAACAGCTCGTCGACTGTGATC10.6097560975609756No Hit
CGTCCACACAGTGAACCCAACCACATCCTTTGGTGCAGAACTGATCCGTCG10.6097560975609756No Hit
GGATAACGCCAAGGCATCTGCAGTTGGCGATTAGAGGAGATGAGGAGCTGG10.6097560975609756No Hit
ATTGTGAACTAATAAAAGAAGAATCATCTTCTTCTCGTAAATAATGTATCT10.6097560975609756No Hit
CCCACAAGTAGTGTTCCTGCTTTAGACATTCTTGTATAAGATTGTAACCAA10.6097560975609756No Hit
GTCTAGAGCTGAGTGAAAGAAGTGGAGACTTATTGTTATTATCATCATGCT10.6097560975609756No Hit
ATCTCCATTCTATCTGATGCTGGTCTTGGAATGGCAATGTTTAGTTTGGGG10.6097560975609756No Hit
GCTGAAGCATACCTCGTTGGATTGTTTGAAGACACCAATCTTTGCGCGATT10.6097560975609756No Hit
CTAGCTATCAGCTCCACAGTCTCGATACGGTCGAGTTACCGCCGAACCTGA10.6097560975609756No Hit
CATATATAAGGATACAACATTAGTCAAACATGTAATCTGAAATGGTATGAT10.6097560975609756No Hit
CCCTTAGTCATCCGGCGGATAGGATTCTCACCTATCAATTCGTTACTCATG10.6097560975609756No Hit
AGATACGTTTCATGAGCTTGCGGCGAATGAGATGATGCGTTGTGCAGAGCA10.6097560975609756No Hit
GGGTTACATGGAGGAGCGGTGGCATCAATTGCAGAGAGAGTAGCGATGGCT10.6097560975609756No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGAA10.6097560975609756No Hit
GATCATTGAGACTGCCTCTTCTATGCCTGTTCCGGAAAACACCTCTTGATT10.6097560975609756No Hit
CTGATACTGGAACAGAACAAAAATAGAGTAAAGGCTCGAGATTCAAGCCAA10.6097560975609756No Hit
GAGAATACATATGCGGCTAAAGTGAGCGAGAAACAAGACTTCTCGGAGAGC10.6097560975609756No Hit
CCTATGAGCAGTCTTCAAAACATTTTGCCTTACATGGAAGCAAAAAACTAG10.6097560975609756No Hit
GCATCACGCATGAGTGAAAGGTTTCGGAGCAAGGGGAATGTGGATCAAAAA10.6097560975609756No Hit
ATACTGATACAACATCGAAAATCACTTCATGAAAAACATTGAAACCAAAAA10.6097560975609756No Hit
CTTCAAAGAGTGCGTAATAGCTCACTGGTCGAGTGACGATGCGCCGAAAAT10.6097560975609756No Hit
GTTGTCTGGTTTTGCCTTCACTGCAAACGGATGGGTCAAATCTTATGGATC10.6097560975609756No Hit
ATCGTGGGGTATTTGTACCTAGCGTACGACTGTATCTCTTCAGCACTTTCT10.6097560975609756No Hit
GAGTGAACTTAGAGACCAGACTCGATAAGGTAATCGCCGAGCCTCTCCACG10.6097560975609756No Hit
ACACCGGAGTGATGGTCATCGAGTTAAAGAAATGGCGAGAAGGAGATTACA10.6097560975609756No Hit
GATTGGATGATGGAAAAACCAGCAAGCCCCTCTTCTTTCAGTCGAGTTACT10.6097560975609756No Hit
CTCTCTGTGATATTGATCGCCGGAGAAGTTACAAGACGGCCTTGTTTCAAG10.6097560975609756No Hit
CAATATTACAACATTGCGAATGAAATTCCAAAGAAGTAGTTACATCTGTCT10.6097560975609756No Hit
CTATATCTACATACCAAATCCAGATCAAAACACAGACAATCAAAAGAAACT10.6097560975609756No Hit
TACCACGACTGTCGAAGATCTGTCTAGCCTTAACAACGGTGATAGTCTGTC10.6097560975609756No Hit
AGACAAGACCAAAATGGTAGATCTAAATGTTTCTAGAGAGATACCACATGT10.6097560975609756No Hit
GTGCAGAAAGTGTAAGAAACAATAATAATGATACTTTACGAGTTTAGTATC10.6097560975609756No Hit
GAATAATATGTTCTCAAAACTTGTATCTAGTTGATGTAACACCTTCTACGT10.6097560975609756No Hit
GTTCCTCTCTGATTGTTTTGTGCGTGCTGAACAGAAAACCACAAAGAGTAT10.6097560975609756No Hit
GTGTGACAAAGAGCAGTGTAGAGGATCTTGATCCGAACCTCACCAGCTTGA10.6097560975609756No Hit
CTTCATTCGAGAGTCTTTGACGGGGAACATGGCGCCGTACTAGACAGTGGT10.6097560975609756No Hit
TACGTGGGTTCGGTCCTCCAAGGCCTGTTAGAGCTCTCTTCAACCTGCTCA10.6097560975609756No Hit
GCTGATTATGATGAATCTGTTACTGAAACTTTGGAGAATGATTGCTTCTGT10.6097560975609756No Hit
CTGTATGCTCCCTGTTGGTGATGGAATCACTATCTGCCGTCGGATCAGTTG10.6097560975609756No Hit
ATCCATGGCCTGGTCGATAGGATCATGGAAGTGATGGCAATACCATTTCTA10.6097560975609756No Hit
CACTTAGATTATAACTATAAGCCACCACAAAAAAAGGTAAAACCTTATAGA10.6097560975609756No Hit
GAGTATTGCTTGGCACATAACCCATATTCGCAACAACAGGCGTCATACCGG10.6097560975609756No Hit
TCTCTTATGCTTGTTGTCTTCTGGTTCAATGCTAAAGAAGATGACCATGAC10.6097560975609756No Hit
CCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTG10.6097560975609756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAC10.6097560975609756No Hit
CCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCACGCCGTTAGGCGC10.6097560975609756No Hit
TAACAGAATATGCCTTGTAATTCGAAATGATATCCATAGCATTTTTGTACT10.6097560975609756No Hit
TCTTCGTGGTGGTCATTGGTGGTGGTGGTGGTGGAGGATTTGCTGCCGGAG10.6097560975609756No Hit
GGTATCAACGCAGAGTACGGGAATGCATTGGATGGATGCCCGGGCATTGAG10.6097560975609756No Hit
ATTAGTTCCTCCCTGTATTTCCTTGGAGGAAGATGATAGCAGCAGCGATGA10.6097560975609756No Hit
CTTTAGTGGCGATGACTTTGGCTATGTAGACATTGCGTTGATTGGATTCTA10.6097560975609756No Hit
ATTTGATCACCACCTTTGAAGGAGAAAAGGATTTGGCCAAGTACTCATTGT10.6097560975609756No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGA10.6097560975609756No Hit
GAAGAGTATGGCTCAAGACTTTTCTTGTCTTCATAATACGAACAATGGCTG10.6097560975609756No Hit
GTTTGGCACCCTAAAGCGGAACTCTTCTGTGCATACCGAAACCTCAAAGCC10.6097560975609756No Hit
CTGGAAGGATGGTCTGAGCGGCAGACACCATCTCAAAGATGGGTTTCTCTC10.6097560975609756No Hit
GTTTAGGATTGTGTTTTGATCACCGATTCAAACATAAACTGGATACAAGAC10.6097560975609756No Hit
AGGTAGCCCACACATGTACAGGTACATGCTTAGTCAGCGAAAGAGAGCTCT10.6097560975609756No Hit
TAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTC10.6097560975609756No Hit
GATAGAGAGAAGAGAATTTGGGGGTTTCGCCCCCGTACTCTGCGTTGATAC10.6097560975609756No Hit
CAGTGATAGCGTTCTCTACAGTTCACCAGACGGTTGCAGCGGTTGACGCTC10.6097560975609756No Hit
TAATAACACTTCAACCATGCAGTTTCAGGTTCTGGAAACATAATACTGTTA10.6097560975609756No Hit
CAAGTGTTTCCTGGCTCTTATTGCAATCTTTTTCTTTTTCAAAATCATAGA10.6097560975609756No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers