##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_96.3410000000a583.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.714285714285715 0.0 0.0 0.0 0.0 0.0 2 34.714285714285715 0.0 0.0 0.0 0.0 0.0 3 33.57142857142857 0.0 0.0 0.0 0.0 0.0 4 35.57142857142857 0.0 0.0 0.0 0.0 0.0 5 35.42857142857143 0.0 0.0 0.0 0.0 0.0 6 36.857142857142854 0.0 0.0 0.0 0.0 0.0 7 38.57142857142857 0.0 0.0 0.0 0.0 0.0 8 31.142857142857142 0.0 0.0 0.0 0.0 0.0 9 29.714285714285715 0.0 0.0 0.0 0.0 0.0 10 31.0 0.0 0.0 0.0 0.0 0.0 11 31.428571428571427 0.0 0.0 0.0 0.0 0.0 12 31.714285714285715 0.0 0.0 0.0 0.0 0.0 13 35.57142857142857 0.0 0.0 0.0 0.0 0.0 14 37.0 0.0 0.0 0.0 0.0 0.0 15 35.0 0.0 0.0 0.0 0.0 0.0 16 32.857142857142854 0.0 0.0 0.0 0.0 0.0 17 31.0 0.0 0.0 0.0 0.0 0.0 18 33.0 0.0 0.0 0.0 0.0 0.0 19 35.285714285714285 0.0 0.0 0.0 0.0 0.0 20 26.142857142857142 0.0 0.0 0.0 0.0 0.0 21 29.714285714285715 0.0 0.0 0.0 0.0 0.0 22 29.857142857142858 0.0 0.0 0.0 0.0 0.0 23 35.0 0.0 0.0 0.0 0.0 0.0 24 32.57142857142857 0.0 0.0 0.0 0.0 0.0 25 31.714285714285715 0.0 0.0 0.0 0.0 0.0 26 38.0 0.0 0.0 0.0 0.0 0.0 27 35.285714285714285 0.0 0.0 0.0 0.0 0.0 28 37.285714285714285 0.0 0.0 0.0 0.0 0.0 29 37.714285714285715 0.0 0.0 0.0 0.0 0.0 30 37.714285714285715 0.0 0.0 0.0 0.0 0.0 31 33.285714285714285 0.0 0.0 0.0 0.0 0.0 32 37.714285714285715 0.0 0.0 0.0 0.0 0.0 33 31.428571428571427 0.0 0.0 0.0 0.0 0.0 34 29.857142857142858 0.0 0.0 0.0 0.0 0.0 35 34.42857142857143 0.0 0.0 0.0 0.0 0.0 36 33.42857142857143 0.0 0.0 0.0 0.0 0.0 37 35.0 0.0 0.0 0.0 0.0 0.0 38 28.571428571428573 0.0 0.0 0.0 0.0 0.0 39 37.0 0.0 0.0 0.0 0.0 0.0 40 36.714285714285715 0.0 0.0 0.0 0.0 0.0 41 27.0 0.0 0.0 0.0 0.0 0.0 42 30.571428571428573 0.0 0.0 0.0 0.0 0.0 43 31.857142857142858 0.0 0.0 0.0 0.0 0.0 44 34.142857142857146 0.0 0.0 0.0 0.0 0.0 45 32.285714285714285 0.0 0.0 0.0 0.0 0.0 46 34.285714285714285 0.0 0.0 0.0 0.0 0.0 47 33.42857142857143 0.0 0.0 0.0 0.0 0.0 48 32.57142857142857 0.0 0.0 0.0 0.0 0.0 49 32.57142857142857 0.0 0.0 0.0 0.0 0.0 50 24.285714285714285 0.0 0.0 0.0 0.0 0.0 51 27.285714285714285 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores warn #Quality Count 25 1.0 26 0.0 27 1.0 28 0.0 29 0.0 30 0.0 31 0.0 32 1.0 33 0.0 34 1.0 35 1.0 36 1.0 37 0.0 38 0.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.857142857142854 14.285714285714285 14.285714285714285 28.57142857142857 2 14.285714285714285 0.0 42.857142857142854 42.857142857142854 3 28.57142857142857 0.0 28.57142857142857 42.857142857142854 4 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 5 14.285714285714285 57.14285714285714 28.57142857142857 0.0 6 57.14285714285714 14.285714285714285 14.285714285714285 14.285714285714285 7 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 8 42.857142857142854 28.57142857142857 0.0 28.57142857142857 9 42.857142857142854 28.57142857142857 28.57142857142857 0.0 10 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 11 42.857142857142854 28.57142857142857 0.0 28.57142857142857 12 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 13 42.857142857142854 14.285714285714285 14.285714285714285 28.57142857142857 14 0.0 14.285714285714285 71.42857142857143 14.285714285714285 15 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 16 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 17 28.57142857142857 14.285714285714285 28.57142857142857 28.57142857142857 18 0.0 28.57142857142857 42.857142857142854 28.57142857142857 19 14.285714285714285 14.285714285714285 57.14285714285714 14.285714285714285 20 28.57142857142857 42.857142857142854 28.57142857142857 0.0 21 42.857142857142854 0.0 28.57142857142857 28.57142857142857 22 0.0 42.857142857142854 28.57142857142857 28.57142857142857 23 14.285714285714285 0.0 57.14285714285714 28.57142857142857 24 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 25 14.285714285714285 28.57142857142857 42.857142857142854 14.285714285714285 26 14.285714285714285 0.0 28.57142857142857 57.14285714285714 27 42.857142857142854 28.57142857142857 28.57142857142857 0.0 28 0.0 28.57142857142857 42.857142857142854 28.57142857142857 29 0.0 28.57142857142857 28.57142857142857 42.857142857142854 30 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 31 28.57142857142857 28.57142857142857 14.285714285714285 28.57142857142857 32 14.285714285714285 14.285714285714285 57.14285714285714 14.285714285714285 33 14.285714285714285 14.285714285714285 57.14285714285714 14.285714285714285 34 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 35 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 36 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 37 14.285714285714285 14.285714285714285 57.14285714285714 14.285714285714285 38 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 39 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 40 28.57142857142857 14.285714285714285 57.14285714285714 0.0 41 42.857142857142854 0.0 28.57142857142857 28.57142857142857 42 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 43 0.0 28.57142857142857 57.14285714285714 14.285714285714285 44 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 45 0.0 28.57142857142857 42.857142857142854 28.57142857142857 46 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 47 28.57142857142857 42.857142857142854 28.57142857142857 0.0 48 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 49 28.57142857142857 28.57142857142857 14.285714285714285 28.57142857142857 50 14.285714285714285 57.14285714285714 28.57142857142857 0.0 51 14.285714285714285 57.14285714285714 14.285714285714285 14.285714285714285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.5 29 1.0 30 0.5 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.5 41 1.0 42 0.5 43 0.0 44 0.5 45 1.0 46 0.5 47 0.0 48 0.5 49 1.0 50 0.5 51 0.0 52 0.5 53 1.0 54 0.5 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.5 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 7.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCCCTTCCTCCCGTAAGATTCATATGGTTGCTTTCATTATTTTGACGACAT 1 14.285714285714285 No Hit GTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGC 1 14.285714285714285 No Hit GTGATGAGGAAGGTTGGTTGGTTCACGCAAACCTGTTGATCCACTTATGAA 1 14.285714285714285 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 1 14.285714285714285 No Hit TCTAAGAAGAGGAAGATTGAGATTCTTAAAGATATAAGTGGCATTATCAAA 1 14.285714285714285 No Hit CTCTAGGAATGTCTGACACATCCGGCATCTCTGCAGTCGAGCCACAACAAG 1 14.285714285714285 No Hit CGTTGCCCATATCTATCCTACAGGTCAACAATTGACGAGGCGTGAGAGCTA 1 14.285714285714285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE