##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_93.3410000000a55d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.25 0.0 0.0 0.0 0.0 0.0 2 35.95 0.0 0.0 0.0 0.0 0.0 3 35.55 0.0 0.0 0.0 0.0 0.0 4 36.85 0.0 0.0 0.0 0.0 0.0 5 36.5 0.0 0.0 0.0 0.0 0.0 6 38.6 0.0 0.0 0.0 0.0 0.0 7 39.1 0.0 0.0 0.0 0.0 0.0 8 39.0 0.0 0.0 0.0 0.0 0.0 9 39.1 0.0 0.0 0.0 0.0 0.0 10 37.0 0.0 0.0 0.0 0.0 0.0 11 37.35 0.0 0.0 0.0 0.0 0.0 12 39.0 0.0 0.0 0.0 0.0 0.0 13 37.85 0.0 0.0 0.0 0.0 0.0 14 36.4 0.0 0.0 0.0 0.0 0.0 15 38.4 0.0 0.0 0.0 0.0 0.0 16 38.15 0.0 0.0 0.0 0.0 0.0 17 39.2 0.0 0.0 0.0 0.0 0.0 18 39.1 0.0 0.0 0.0 0.0 0.0 19 39.1 0.0 0.0 0.0 0.0 0.0 20 38.8 0.0 0.0 0.0 0.0 0.0 21 38.7 0.0 0.0 0.0 0.0 0.0 22 37.65 0.0 0.0 0.0 0.0 0.0 23 38.3 0.0 0.0 0.0 0.0 0.0 24 39.3 0.0 0.0 0.0 0.0 0.0 25 38.7 0.0 0.0 0.0 0.0 0.0 26 38.8 0.0 0.0 0.0 0.0 0.0 27 37.45 0.0 0.0 0.0 0.0 0.0 28 37.45 0.0 0.0 0.0 0.0 0.0 29 38.15 0.0 0.0 0.0 0.0 0.0 30 37.1 0.0 0.0 0.0 0.0 0.0 31 37.85 0.0 0.0 0.0 0.0 0.0 32 37.45 0.0 0.0 0.0 0.0 0.0 33 36.55 0.0 0.0 0.0 0.0 0.0 34 38.35 0.0 0.0 0.0 0.0 0.0 35 38.05 0.0 0.0 0.0 0.0 0.0 36 38.35 0.0 0.0 0.0 0.0 0.0 37 39.2 0.0 0.0 0.0 0.0 0.0 38 38.7 0.0 0.0 0.0 0.0 0.0 39 37.5 0.0 0.0 0.0 0.0 0.0 40 38.25 0.0 0.0 0.0 0.0 0.0 41 38.55 0.0 0.0 0.0 0.0 0.0 42 37.9 0.0 0.0 0.0 0.0 0.0 43 38.2 0.0 0.0 0.0 0.0 0.0 44 38.5 0.0 0.0 0.0 0.0 0.0 45 37.8 0.0 0.0 0.0 0.0 0.0 46 37.15 0.0 0.0 0.0 0.0 0.0 47 38.0 0.0 0.0 0.0 0.0 0.0 48 38.1 0.0 0.0 0.0 0.0 0.0 49 36.75 0.0 0.0 0.0 0.0 0.0 50 36.7 0.0 0.0 0.0 0.0 0.0 51 35.8 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1206 1 0.0 1206 2 3.0 1206 3 0.0 1206 4 0.0 1206 5 -3.0 1206 6 0.0 1206 7 0.0 1206 8 1.0 1206 9 1.0 1206 10 0.0 1206 11 0.0 1206 12 -1.0 1206 13 -1.0 1206 14 0.0 1206 15 4.0 1206 16 1.0 1206 17 1.0 1206 18 0.0 1206 19 0.0 1206 20 -1.0 1206 21 -1.0 1206 22 0.0 1206 23 -1.0 1206 24 0.0 1206 25 -1.0 1206 26 0.0 1206 27 0.0 1206 28 4.0 1206 29 NaN 1206 30 NaN 1206 31 1.0 1206 32 1.0 1206 33 3.0 1206 34 0.0 1206 35 1.0 1206 36 1.0 1206 37 1.0 1206 38 3.0 1206 39 3.0 1206 40 4.0 1206 41 1.0 1206 42 4.0 1206 43 4.0 1206 44 0.0 1206 45 1.0 1206 46 1.0 1206 47 0.0 1206 48 0.0 1206 49 0.0 1206 50 0.0 1206 51 -1.0 2214 1 0.0 2214 2 -3.0 2214 3 0.0 2214 4 0.0 2214 5 3.0 2214 6 0.0 2214 7 0.0 2214 8 -1.0 2214 9 -1.0 2214 10 0.0 2214 11 0.0 2214 12 1.0 2214 13 1.0 2214 14 0.0 2214 15 -4.0 2214 16 -1.0 2214 17 -1.0 2214 18 0.0 2214 19 0.0 2214 20 1.0 2214 21 1.0 2214 22 0.0 2214 23 1.0 2214 24 0.0 2214 25 1.0 2214 26 0.0 2214 27 0.0 2214 28 -4.0 2214 29 NaN 2214 30 NaN 2214 31 -1.0 2214 32 -1.0 2214 33 -3.0 2214 34 0.0 2214 35 -1.0 2214 36 -1.0 2214 37 -1.0 2214 38 -3.0 2214 39 -3.0 2214 40 -4.0 2214 41 -1.0 2214 42 -4.0 2214 43 -4.0 2214 44 0.0 2214 45 -1.0 2214 46 -1.0 2214 47 0.0 2214 48 0.0 2214 49 0.0 2214 50 0.0 2214 51 1.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 31 1.0 32 1.0 33 0.0 34 0.0 35 1.0 36 2.0 37 2.0 38 3.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.0 25.0 25.0 30.0 2 15.0 35.0 20.0 30.0 3 10.0 35.0 25.0 30.0 4 5.0 15.0 55.00000000000001 25.0 5 10.0 35.0 45.0 10.0 6 20.0 45.0 25.0 10.0 7 15.0 45.0 20.0 20.0 8 15.0 50.0 25.0 10.0 9 30.0 15.0 20.0 35.0 10 10.0 35.0 45.0 10.0 11 35.0 20.0 20.0 25.0 12 10.0 40.0 15.0 35.0 13 15.0 20.0 40.0 25.0 14 20.0 25.0 25.0 30.0 15 10.0 55.00000000000001 20.0 15.0 16 25.0 30.0 25.0 20.0 17 30.0 25.0 25.0 20.0 18 15.0 30.0 20.0 35.0 19 15.0 20.0 45.0 20.0 20 30.0 30.0 25.0 15.0 21 15.0 30.0 25.0 30.0 22 20.0 40.0 15.0 25.0 23 15.0 25.0 35.0 25.0 24 10.0 20.0 40.0 30.0 25 25.0 35.0 25.0 15.0 26 30.0 50.0 20.0 0.0 27 30.0 35.0 20.0 15.0 28 15.0 25.0 45.0 15.0 29 20.0 20.0 20.0 40.0 30 10.0 30.0 30.0 30.0 31 20.0 30.0 30.0 20.0 32 35.0 25.0 15.0 25.0 33 15.0 25.0 35.0 25.0 34 20.0 20.0 35.0 25.0 35 15.0 30.0 35.0 20.0 36 10.0 25.0 20.0 45.0 37 10.0 45.0 25.0 20.0 38 20.0 20.0 40.0 20.0 39 5.0 40.0 25.0 30.0 40 5.0 10.0 55.00000000000001 30.0 41 10.0 30.0 20.0 40.0 42 30.0 35.0 15.0 20.0 43 10.0 25.0 40.0 25.0 44 15.0 25.0 15.0 45.0 45 25.0 20.0 25.0 30.0 46 15.0 25.0 25.0 35.0 47 25.0 25.0 20.0 30.0 48 15.0 15.0 60.0 10.0 49 20.0 25.0 10.0 45.0 50 5.0 25.0 45.0 25.0 51 15.0 25.0 40.0 20.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 1.0 29 2.0 30 1.0 31 0.0 32 0.5 33 1.0 34 1.0 35 1.0 36 1.0 37 1.0 38 1.5 39 2.0 40 2.5 41 3.0 42 3.0 43 3.0 44 2.5 45 2.0 46 1.5 47 1.0 48 1.5 49 2.0 50 1.0 51 0.0 52 0.5 53 1.0 54 0.5 55 0.0 56 0.5 57 1.0 58 0.5 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 20.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAATGTACCTCTCGATGATAATCAGACTATCACTGACGATACCAGCTGTCT 1 5.0 No Hit TCTTTAGACAAACCAGCTTTCAAAATAGCAGGAGTCTTCTCAACCTGTCTC 1 5.0 No Hit CAATCGCAGAGAATGAGTCTAATCATGGCAAGAACCAAACACCTGTCTCTT 1 5.0 No Hit GGAGATTGTTCATGACTCTGGATTCAGTAATTCCCCTTTGCAGCCTGTCTC 1 5.0 No Hit CGCTAATAGAGCAAGAACAACATTGGTCAATCTACAAAGCTGTCTCTTATA 1 5.0 No Hit ACCTAACGTTGAGTTAAGGCGCCCAAATGCACGCTCATCAGATACCATAAA 1 5.0 No Hit ACCCTCGAGAACTAATGTTGTCACCATAACAGCTGTCTCTTATACACATCT 1 5.0 No Hit GCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG 1 5.0 No Hit TTATTACATTAGCAAAAACGAAAAAAATCAAAACCCAAACCACTATCTCTG 1 5.0 No Hit TACTTGTGCTGTTACACAAGTCTGTTAAGTTTTTAAGGACTCTTCAATGAT 1 5.0 No Hit CCTATTATAGGCACAGTTGGTGGTTACTGGGGTAATCGCTCAAAGGCAATA 1 5.0 No Hit GAGCTGAATTTGTTACCGAATGATAAAACCAAATGATCATGGTCACACCGG 1 5.0 No Hit AGACAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAATCCGCGGCAT 1 5.0 No Hit GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACT 1 5.0 No Hit ATCTTAATGTGATTCTTCCAGCGAAGCCTCCCTGTCTCTTATACACATCTC 1 5.0 No Hit TTTCATACCTCAAGAGACTAAAACTAGTTTGAGGAGATTTAGAGGAGTGAT 1 5.0 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 1 5.0 No Hit ACATATTAGATATGAAGCGACTTTTAGTTGCCTTTTATATCTTGTGTTCAA 1 5.0 No Hit CAACAAGTCTCTCCTTCATCTTGTGGTACTTCTTAGGATCTGTCTCTTATA 1 5.0 No Hit TACTCCAAGGACGAACGCTTCGCTGTGGCTTTGATCCCTCCGGCCAAAGCT 1 5.0 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 5.0 31 0.0 0.0 5.0 32 0.0 0.0 10.0 33 0.0 0.0 15.0 34 0.0 0.0 15.0 35 0.0 0.0 15.0 36 0.0 0.0 15.0 37 0.0 0.0 15.0 38 0.0 0.0 15.0 39 0.0 0.0 15.0 >>END_MODULE >>Kmer Content pass >>END_MODULE