##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_92.3410000000a540.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.55 0.0 0.0 0.0 0.0 0.0 2 32.95 0.0 0.0 0.0 0.0 0.0 3 35.25 0.0 0.0 0.0 0.0 0.0 4 35.1 0.0 0.0 0.0 0.0 0.0 5 35.6 0.0 0.0 0.0 0.0 0.0 6 35.3 0.0 0.0 0.0 0.0 0.0 7 36.45 0.0 0.0 0.0 0.0 0.0 8 36.55 0.0 0.0 0.0 0.0 0.0 9 37.8 0.0 0.0 0.0 0.0 0.0 10 36.5 0.0 0.0 0.0 0.0 0.0 11 37.55 0.0 0.0 0.0 0.0 0.0 12 37.0 0.0 0.0 0.0 0.0 0.0 13 36.1 0.0 0.0 0.0 0.0 0.0 14 34.85 0.0 0.0 0.0 0.0 0.0 15 34.4 0.0 0.0 0.0 0.0 0.0 16 36.45 0.0 0.0 0.0 0.0 0.0 17 38.1 0.0 0.0 0.0 0.0 0.0 18 36.2 0.0 0.0 0.0 0.0 0.0 19 37.9 0.0 0.0 0.0 0.0 0.0 20 34.95 0.0 0.0 0.0 0.0 0.0 21 37.55 0.0 0.0 0.0 0.0 0.0 22 37.1 0.0 0.0 0.0 0.0 0.0 23 37.55 0.0 0.0 0.0 0.0 0.0 24 36.15 0.0 0.0 0.0 0.0 0.0 25 36.6 0.0 0.0 0.0 0.0 0.0 26 35.4 0.0 0.0 0.0 0.0 0.0 27 36.7 0.0 0.0 0.0 0.0 0.0 28 36.75 0.0 0.0 0.0 0.0 0.0 29 36.3 0.0 0.0 0.0 0.0 0.0 30 36.25 0.0 0.0 0.0 0.0 0.0 31 37.85 0.0 0.0 0.0 0.0 0.0 32 38.2 0.0 0.0 0.0 0.0 0.0 33 35.7 0.0 0.0 0.0 0.0 0.0 34 36.8 0.0 0.0 0.0 0.0 0.0 35 34.1 0.0 0.0 0.0 0.0 0.0 36 37.95 0.0 0.0 0.0 0.0 0.0 37 37.25 0.0 0.0 0.0 0.0 0.0 38 35.05 0.0 0.0 0.0 0.0 0.0 39 34.1 0.0 0.0 0.0 0.0 0.0 40 36.1 0.0 0.0 0.0 0.0 0.0 41 37.35 0.0 0.0 0.0 0.0 0.0 42 37.6 0.0 0.0 0.0 0.0 0.0 43 34.65 0.0 0.0 0.0 0.0 0.0 44 35.55 0.0 0.0 0.0 0.0 0.0 45 36.25 0.0 0.0 0.0 0.0 0.0 46 34.85 0.0 0.0 0.0 0.0 0.0 47 35.95 0.0 0.0 0.0 0.0 0.0 48 37.4 0.0 0.0 0.0 0.0 0.0 49 36.75 0.0 0.0 0.0 0.0 0.0 50 36.5 0.0 0.0 0.0 0.0 0.0 51 37.2 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2215 1 0.0 2215 2 0.0 2215 3 3.0 2215 4 0.0 2215 5 0.0 2215 6 9.5 2215 7 0.0 2215 8 -5.5 2215 9 0.0 2215 10 -12.5 2215 11 0.0 2215 12 -12.5 2215 13 -12.5 2215 14 -13.5 2215 15 12.5 2215 16 5.5 2215 17 0.0 2215 18 3.0 2215 19 -3.0 2215 20 -12.5 2215 21 -4.0 2215 22 -5.5 2215 23 0.0 2215 24 0.0 2215 25 12.5 2215 26 -9.5 2215 27 -12.5 2215 28 -9.5 2215 29 -5.5 2215 30 -3.0 2215 31 3.0 2215 32 0.0 2215 33 0.0 2215 34 0.0 2215 35 0.0 2215 36 -2.5 2215 37 -5.5 2215 38 -13.5 2215 39 -5.5 2215 40 0.0 2215 41 0.0 2215 42 0.0 2215 43 -12.5 2215 44 -12.5 2215 45 0.0 2215 46 12.5 2215 47 0.0 2215 48 -2.5 2215 49 0.0 2215 50 -12.5 2215 51 -5.5 1215 1 0.0 1215 2 0.0 1215 3 -3.0 1215 4 0.0 1215 5 0.0 1215 6 -9.5 1215 7 0.0 1215 8 5.5 1215 9 0.0 1215 10 12.5 1215 11 0.0 1215 12 12.5 1215 13 12.5 1215 14 13.5 1215 15 -12.5 1215 16 -5.5 1215 17 0.0 1215 18 -3.0 1215 19 3.0 1215 20 12.5 1215 21 4.0 1215 22 5.5 1215 23 0.0 1215 24 0.0 1215 25 -12.5 1215 26 9.5 1215 27 12.5 1215 28 9.5 1215 29 5.5 1215 30 3.0 1215 31 -3.0 1215 32 0.0 1215 33 0.0 1215 34 0.0 1215 35 0.0 1215 36 2.5 1215 37 5.5 1215 38 13.5 1215 39 5.5 1215 40 0.0 1215 41 0.0 1215 42 0.0 1215 43 12.5 1215 44 12.5 1215 45 0.0 1215 46 -12.5 1215 47 0.0 1215 48 2.5 1215 49 0.0 1215 50 12.5 1215 51 5.5 >>END_MODULE >>Per sequence quality scores pass #Quality Count 28 1.0 29 2.0 30 1.0 31 0.0 32 0.0 33 1.0 34 0.0 35 1.0 36 1.0 37 2.0 38 6.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.0 30.0 25.0 15.0 2 25.0 25.0 30.0 20.0 3 15.0 25.0 40.0 20.0 4 20.0 20.0 45.0 15.0 5 15.0 40.0 35.0 10.0 6 30.0 35.0 20.0 15.0 7 20.0 45.0 10.0 25.0 8 30.0 30.0 40.0 0.0 9 40.0 15.0 15.0 30.0 10 15.0 30.0 45.0 10.0 11 50.0 35.0 15.0 0.0 12 20.0 15.0 50.0 15.0 13 35.0 20.0 35.0 10.0 14 15.0 40.0 25.0 20.0 15 10.0 45.0 30.0 15.0 16 35.0 15.0 30.0 20.0 17 5.0 40.0 30.0 25.0 18 30.0 30.0 15.0 25.0 19 35.0 30.0 25.0 10.0 20 10.0 35.0 30.0 25.0 21 30.0 15.0 25.0 30.0 22 15.0 30.0 30.0 25.0 23 10.0 55.00000000000001 25.0 10.0 24 30.0 30.0 20.0 20.0 25 35.0 20.0 25.0 20.0 26 25.0 30.0 20.0 25.0 27 5.0 30.0 45.0 20.0 28 5.0 20.0 45.0 30.0 29 15.0 15.0 55.00000000000001 15.0 30 15.0 15.0 55.00000000000001 15.0 31 10.0 20.0 25.0 45.0 32 25.0 25.0 25.0 25.0 33 35.0 25.0 30.0 10.0 34 15.0 30.0 25.0 30.0 35 15.0 30.0 30.0 25.0 36 25.0 10.0 50.0 15.0 37 5.0 35.0 25.0 35.0 38 10.0 30.0 35.0 25.0 39 15.0 40.0 20.0 25.0 40 5.0 30.0 25.0 40.0 41 15.0 20.0 30.0 35.0 42 20.0 25.0 25.0 30.0 43 10.0 25.0 35.0 30.0 44 10.0 25.0 40.0 25.0 45 5.0 20.0 45.0 30.0 46 25.0 20.0 35.0 20.0 47 15.0 25.0 40.0 20.0 48 15.0 25.0 25.0 35.0 49 20.0 25.0 35.0 20.0 50 15.0 40.0 20.0 25.0 51 10.0 25.0 35.0 30.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.5 33 1.0 34 0.5 35 0.0 36 2.5 37 5.0 38 4.0 39 3.0 40 4.5 41 6.0 42 3.5 43 1.0 44 1.5 45 2.0 46 1.0 47 0.0 48 0.0 49 0.0 50 0.5 51 1.0 52 0.5 53 0.0 54 0.5 55 1.0 56 0.5 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 20.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGCCGGG 1 5.0 No Hit ATCTGATTCTGGGAAGACTTCCAAAGTCGTTACCATCACTGATTGTGGTCA 1 5.0 No Hit ATATTCAACATTCGTGTAAAGAGTGTAATCCAAATTATCATCACCGACATC 1 5.0 No Hit TGTTTGCTCAATTCTCAGAGCTTGTGAATGATTTCTACAACAACTGTCTCT 1 5.0 No Hit CGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTACTGTATCTTATA 1 5.0 No Hit GATACATAGGTTCAAAAGAAGAAGAGCTTTGAGTTGCAATGCAGACAATGA 1 5.0 No Hit CATAAAAGGTTCGTACTTGATAAAGATGCAAGGATCCCACATGGTAACGGT 1 5.0 No Hit GCCTTCAAGTGAGCAGCAGCCTTGTCTTTGTCAGTGAAGACACCAGTAGAC 1 5.0 No Hit AAAGTTGACGGTAAGTACCAGTACGAACTTCGTCGATAACCTGTCTCTTAT 1 5.0 No Hit ACAGAGAGGGATATCTACACTGGAGACAAGCTTGAGATTATGATACTTAAG 1 5.0 No Hit AGCTAAGAGTGTTAATCCGTTTAGACTTATCGGAGTCTGTCTCTTATACAC 1 5.0 No Hit GAGGAAATAAGTGCTGAGGTAATAAATCTTCGATCCTGTCTCTTATACACA 1 5.0 No Hit GTCTGAGTATGTTTCTGTCTCTCCGTACTTACTTATTCACGGATCTGAGAA 1 5.0 No Hit CCTCTGCATCAGTCATTTTCACATCACTGTCCTTTGAGACACTCTTAGCAC 1 5.0 No Hit TGTCCGAGTTGTAATTTGAAGAAGGTATCTTTGGGTCTGGCTCTTGTCTAT 1 5.0 No Hit GCGGAGATGAAAGATCAAGCTCATCGTCATCAACATCACCAACACTCTTCT 1 5.0 No Hit TTGTTTGGATGCTTAACATACCCCTCCTCAGTGACTGTCTCTTATACACAT 1 5.0 No Hit GGAAGTCTCTGCTTGGCCGTATTTGTTTTTTTGCTTATTAACCATATGATC 1 5.0 No Hit AATAAGATGTGGGATGTGTTGGAACGTATCACAACCTCTTTATCCTGTCTC 1 5.0 No Hit TTTCTAATGAAGAGCAAAACGCTGCCGTTTTCGAAGAAAATCCTTCTCTCT 1 5.0 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 5.0 36 0.0 0.0 10.0 37 0.0 0.0 15.0 38 0.0 0.0 15.0 39 0.0 0.0 15.0 >>END_MODULE >>Kmer Content pass >>END_MODULE