FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_88.3410000000a50c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_88.3410000000a50c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTATCCCATGCTAATGTATCCAGAGCGTCGGCATGCTTTGAGCACTCT13.8461538461538463No Hit
GACATGAGTAACGATAAATCCTGTGAAAAACACGATCGCTGTCTCTTATAC13.8461538461538463No Hit
GTATCTCATTTGAAATGTCATGATGGATGAGAGGAAAAAACGGTGGCCAGT13.8461538461538463No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACTGTCTCTTATA13.8461538461538463No Hit
GACTGAAAGAAGAGGAGAGGTGGCGACCATTTTCAAGAACCAAAAAGACTG13.8461538461538463No Hit
CTACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACTGTCTCTTATAC13.8461538461538463No Hit
CCATGAAATCAGCCAAAACGGTAAGTGCACCGATACAAAACCTGTCTCTTA13.8461538461538463No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG13.8461538461538463No Hit
AGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGCTGTCTCTT13.8461538461538463No Hit
CTTTAGGAATCTTCAGACAAGGTACGAAATAACTCGACCTGACTCTTATAT13.8461538461538463No Hit
GTATCACACACTGAATACATAGGTGTGTGACTGTCTCTTATACACATCTCC13.8461538461538463No Hit
AACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTG13.8461538461538463No Hit
GGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTCTCTTATG13.8461538461538463No Hit
GTCATAGGGATCTTTTCACCTTTCCCTCACGGTCTGTCTCTATACACATCT13.8461538461538463No Hit
CTTCACACAGGTGTAATCTAACATACTCAATTCTGATAGATAGCAACAAAG13.8461538461538463No Hit
GGAAATTAACTACTATGAGGAAAGGGTATGCATCTCATATTTCTCTGTCTC13.8461538461538463No Hit
GAAAGAGTGATACATAGGATCAGATCTCCAAAGAAGAGCCATGAGAAGATG13.8461538461538463No Hit
GTTCCAAAGATACTTAATGAAGAAGAAAAACTAATGAAGCTGTCTCTTATA13.8461538461538463No Hit
ATAGAAGCGACGTCGTTTTGTTAAAATTCCTAAGAGCTAGGGAGTTCAAGG13.8461538461538463No Hit
GAGTACGGGATATCCAGTGCCATTGCAATTTTCTCGAGAAAGTGACGGAAA13.8461538461538463No Hit
GTATCAACGCAGAGTACGGGAATGCATTGGATCTGTCTCTTATACACATCT13.8461538461538463No Hit
GTTCCAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATTCTGTCTCTTATAC13.8461538461538463No Hit
TAACAAGTAGAAGTAATAACCATAGCTACAAAAAATTCTCTGCACTCTTAG13.8461538461538463No Hit
AACTGAAACATCTAAGTAGCTAAAGGAAGGGAAATCAACCTGTCTCTTATA13.8461538461538463No Hit
CGATAGTTTATCTGCATTGGCTTGGATTGCTTTCAATGGAAAAGATTGTGG13.8461538461538463No Hit
GCCGAATGGTGAAGCCGATGGACAACAGGTAGAAATTCCTGTACCACCAAA13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)