FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_87.3410000000a4fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_87.3410000000a4fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGACCTGTCT11.282051282051282No Hit
GTGCATACCTAATTTCACTGCTGCCCTGAAATAAATGTACTCTGTACAAAC11.282051282051282No Hit
CTGTTTGATTGTTGCTTCCTGTTATTTTAGTTGTTAAAGGTGGCATTCTGT11.282051282051282No Hit
ATCCACAATAAATCGAATGAAACCTGGCGAAAAAGAAGCTGTCTCTTATAC11.282051282051282No Hit
GAGTAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACCTGTCTCTTATAC11.282051282051282No Hit
GTATCAACGCAGAGTACGGGATCCAATTCCAATTTCAAAATCAGATCCAAA11.282051282051282No Hit
GTTCTATAAATTGCTCGAAGTTCTTGCTAGAACAATAAGAAAACAAGTGAT11.282051282051282No Hit
TAATATGGGGATGTTAAAAGTATCAAGGTTATCCTATCTCTTGCTCTTGTA11.282051282051282No Hit
GAAACAAGATACACCACCACTTATTGTCTTCAAACATTATTGCACTTTAAC11.282051282051282No Hit
GTCTTAGGATTTCTTGTTTGGGCTCATCATATGTTTACCTGTCTCTTATAC11.282051282051282No Hit
CTAAGAACCTTGAAGCTGCTCAAAAATTTGTAGACTTCCTTGTTTCCACTG11.282051282051282No Hit
CATCCAATCAGTAGCTCTACCTCTAATAAACCTGTCTCTTATACACATCTC11.282051282051282No Hit
AAGCAGAGCGTCGCCGAGCATTCGGCAGAGCTCTTCGCGTGTCCGGTCCTC11.282051282051282No Hit
CTGACGCACCATAGCGGCGTGAATGGCCCCTGTCTCTTATACACATCTCCG11.282051282051282No Hit
AGCATAAGAAGGTTAAGCTCGCTGTTCTTCAGCTGTCTCTTATACACATCT11.282051282051282No Hit
GGCTCAAGGCCACCAGCGCACAGGCCAACAACCCCAGTACGCGATCCTCAT11.282051282051282No Hit
GTCGAAGATACCAGCTGGCTGCAACTGTTTATTAAAAACACAGCCTGTCTC11.282051282051282No Hit
GAATAAAATCAAAAAAGAAATCAAATAAAGAAAAGAGAGTTAAAATAAAGA11.282051282051282No Hit
AACCAAAACTCATGTTTGTTCACTCCAAACACAAACACAGCAGTAATCAAA11.282051282051282No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC11.282051282051282No Hit
GTAATAATATCGATTCATAATGGATTCATATCGATACCTGTCTCTTATACA11.282051282051282No Hit
CCCTAAAATAAGAACTTCAAAAGAAAATAATCCTGTTACCCCCAAAGTATC11.282051282051282No Hit
CTTCCCACATAACATAATGAGCAGCCAAAATGTCATAACACAACAAAATGG11.282051282051282No Hit
ATGAGCACAGAGACTAGATACGAAAATGGATCTGTCTCTTATACACATCTC11.282051282051282No Hit
CTCTAGAACAGTGGGAGAAATAAGTAACACACAAGGGTAATTGCTAATTAC11.282051282051282No Hit
ACCTGTATTTGTATCTCTTTATGCGCCAATTGAAGGTGATAAAGAATGGTT11.282051282051282No Hit
TTCCATATCATTGTTCATTTAAAGGCACTCGAGGATGTTAACCTGTCTCTT11.282051282051282No Hit
GATAATTTAGTAGGTGGGATTTTCCATTTAGCCCATGGCATACTTCTGAGT11.282051282051282No Hit
ACATCTATGTGAGCTTTTTTCCGTCTGGGTACTTTCACAGAAAATTTTGCC11.282051282051282No Hit
TTGTTAGAGTGTTTGGCGTGAGTTTTCTTCTTACTTGTTTGTTCAGAGCTT11.282051282051282No Hit
AATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAG11.282051282051282No Hit
GGGTAATCCCGCCTGACCTGGGGTCGCCCCGTACTCTGCGTTGATCTGTCT11.282051282051282No Hit
CTTGTAATATTTAGCTTTAGATGAAATCTATCACCTTATTAGAGTTGCATT11.282051282051282No Hit
ACTTAGATGTTTCCCCGTACTCTGCGTTGATACCTGTCTCATATACAAATA11.282051282051282No Hit
CCAGTATTCCCTTTTGCTGCCTCCAAAAGGCCTGTCCCTGCAGACATGAGA11.282051282051282No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG11.282051282051282No Hit
CCTACTTATCAACATGCCTAGTACCATTAAATAACCTTCAGATACGGGAAT11.282051282051282No Hit
GCTATAAGTAATGCAACTATGAATCTCATGGCTGTCTCTTATACACATCTC11.282051282051282No Hit
CACTTAATGATGGTCAAAAGAATTAAATAGAGAAATGATTGCGTGGAATAG11.282051282051282No Hit
GCTAGAGTCTCTGCCAAAACCAAGTATCTGATCATGTATCTCAGCTTCAGA11.282051282051282No Hit
ATGCAAAAGACATATATGGTAGAATCGTAACCATAGAATACGACCCTAATC11.282051282051282No Hit
CGTAATGGTAGTGTATTTTGGCAAGTACGTGCACTAAGAATCACACCTGTC11.282051282051282No Hit
AGTATGCTCCCCATACATAGACTACGTACAGGTCTGTCTCTTATACACATC11.282051282051282No Hit
GAATGGAATGGAACGGTACGGAATAGAATGGAATGGAATGGAATGCAATGG11.282051282051282No Hit
ATGATAACAATGCATCGTAAAACTTTCAGAAGGAAAGGCTGTCTCTTATAC11.282051282051282No Hit
ATCCACAATAAATCGAATGCAACCTGGCGAAAAAGAAGTGAAACCTGTCTC11.282051282051282No Hit
ATCAATTACAAAGCCAAATTAACAGATGAATATGAGGACTGTCTCTTATAC11.282051282051282No Hit
CGATAGTGAAGAGTAACGTGAGTGAAAGGTGAAAAGTAAGTGTATGACTGG11.282051282051282No Hit
CCCGTGAATCGAAAAAATATCATATCCTCCTGTCTCTTATACACATCTCCG11.282051282051282No Hit
ATACCAGTGAATGCCTTTTATTTAAAGGCATCGGCTGCATATCATGAGTTA11.282051282051282No Hit
TCGATCAGCAAGAAGAATATACGGCTCTTAGAGAATGTATCACTTTGGGAA11.282051282051282No Hit
GTGCTTATTCTGTCGTAACGTCAAAGTACTAACGTATTAGGTTAATACCCT11.282051282051282No Hit
CCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACACTGTCTCTTAT11.282051282051282No Hit
TATCAACGCAGAGTACGGGGCAATATTATCAGAAATGATGCGTATGAGACC11.282051282051282No Hit
GACAATATCAAGACTGACAATATTGTCGCTTGACTTGCTTATTTTGCCATT11.282051282051282No Hit
TATCAACGCAGAGTACGGGGAGCATATACCATGGTTAAGGAGACGAAACTA11.282051282051282No Hit
AATAAGGAGAGAGTATATGTCATATGTTTGCAAATTTCAAGGGATCCTGCC11.282051282051282No Hit
AATCAAAGCTTGCTTTGACTTCTACAAAGACTGTCTCTTATACACATCTCC11.282051282051282No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCTGTCTCTTATACACATC11.282051282051282No Hit
TATCAAAGCAGAGTACGGGCAATCATGTTTCAACGTTTAGAAGCTTTTCCT11.282051282051282No Hit
TAGTAAAACTACAACAATAGATACACCATGGAATTCTCTCTAACGGTCCTT11.282051282051282No Hit
GGAGAATTGAACATAAAGTTCCATAAGAAGCCCAATCTGTCTCTTATACAC11.282051282051282No Hit
GAGGAAAAGAAAGAAGAGGATTCAGAGAAGGAGGAAGCAGGAGGGTCTGGT11.282051282051282No Hit
GTTTTTCCATCATCCAATCCACAATAAATCGAATGCTGTCTCTTATACACA11.282051282051282No Hit
GGTTAAGAATAGGAGTTCCGAACAGAGAACCTGTCTCTTATACACATCTCC11.282051282051282No Hit
AGATGATTCGTCTAACATGTTCTTTTGTAACTCCGTATAGAGTGTCCTCTG11.282051282051282No Hit
GGTTATAATGGACCGAACAGCAAAATGTTGCAGTATTTTCTGACTGTCTCT11.282051282051282No Hit
CATCAGGCCCCACGACATCAACACCACCAACACTCAAAAAGTGAAAAGGTA11.282051282051282No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC11.282051282051282No Hit
GACCTAGATGGTGAAGAATATCAAGTTGAAAGAGACCTGTCTCTTATACAC11.282051282051282No Hit
GATATCAGAAGTGAGAATGCTGACATGAGTAACGATAAATCCCTGTCTCAT11.282051282051282No Hit
TCTTAAAAGAGTCCTCGACTGTATTGGAAAAACAAAAAGTTCAATATGGCG11.282051282051282No Hit
AATTAGAGGGAGGAGGATGGTGTGACAACATTAGGAACTGTGTATACCCTG11.282051282051282No Hit
GAGTTAGCCGATGCTTATTCCCCAGATACCGTCATTGCTTCTTCTCTGGGA11.282051282051282No Hit
GTACAGACTCTCACCTAAGCAGACTAAATTCCTAAGTTCCTTGGAGTCCCG11.282051282051282No Hit
GTGAAGCAGAATTCACCAAGTGTTGGATTGTTCACCCACCTGTCTCTTATA11.282051282051282No Hit
ATGCAATACACACCAGGAGATGTAGATAACTTGTTTTCTTTCAGTAACAAC11.282051282051282No Hit
CGAAAGGGTTTCATGGAAAAAGTGATCTGCCATGATTTATACGGTTTCTAA11.282051282051282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTT50.045.033
TTCATTT50.045.014
TTGTTCA50.045.011
CATTGTT50.045.09
GGCACTC50.045.024
ACTCGAG50.045.027
GAGGATG50.045.031
AGGATGT50.045.032
TGTCTCT50.045.044
CCTGTCT50.045.042
GATGTTA50.045.034
TGTTCAT50.045.012
GTTCATT50.045.013
AACCTGT50.045.040
CATTTAA50.045.016
ACCTGTC50.045.041
TGTTAAC50.045.036
CGAGGAT50.045.030
TAACCTG50.045.039
ATTGTTC50.045.010
Produced by FastQC (version 0.11.2)