FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_85.3410000000a4c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_85.3410000000a4c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATATTATTCTTGGGAGCTGCTAAATTAGCTGAAGATGAAGAAGGTG13.225806451612903No Hit
GGTAGCGGGCTTCGGCGCTGTTCGGCTCCGGCTTGAAGAACGGCCGGTTCT13.225806451612903No Hit
CTACATGCTTCAAGGTGCTAGTTCGTTTTTGCCAGTGATGGCCCTTGCCCC13.225806451612903No Hit
TCCGTGTTTCCAAGAAACCTAATATAACCGTAACCAATCCTGCCAATAGCT13.225806451612903No Hit
CTGTACGCCTATCCGTGCCTTTGAATATCATATGCTCCAAAAAATGAGCCG13.225806451612903No Hit
GCAGTGGACTGCTCATCGTTGATACTAAACATGGCTGATTGTTTGTCGTTT13.225806451612903No Hit
CCTGAATGAGGATATAAACGAAGAATGGATATCTGACAAAACCCGATTCCT13.225806451612903No Hit
GCCATGGAATATGCTGCTGGTGGTGAACTATTCGAGCGTATATGCAGTGCT13.225806451612903No Hit
ATCCCAAACCGTGAAGCTGATGTTCTTGTACTCAACCGTCTCCACATTAAA13.225806451612903No Hit
CTTCTAATCTTATGTCTAATGTCTCTAATACCGGGTTAGGAGGCTGGAACG13.225806451612903No Hit
CCTATGGTCACGTCCTCATTGCTAAACATCCTGAAACTGTTGTTCTTGAGG13.225806451612903No Hit
GGCCAATGGACTCGGCGTGCTGGAAGGCGAAGCGGAGGACGCGCTCGGAAG13.225806451612903No Hit
CCTCAAGAGACTAAAACTAGTTTGAGGAGATTTAGAGGAGTGTTTGGTTCT13.225806451612903No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTTCACCCACCA13.225806451612903No Hit
ACTATGGACAGTGTCAGAACTGGACCTTATGGTCAAAATCTTCAGGCCTGA13.225806451612903No Hit
ATGCTCTTCTCTCGGATGAAGCCCTTGCAACTGTCCCGTTTCTGATTCTTG13.225806451612903No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTGATCCCCC13.225806451612903No Hit
CCTGAGAATCTCTTTCACAGAAAGCAGCAATGATCAAGAACTGAATGATTC13.225806451612903No Hit
ATTCTCTCCTTATCCCCAAAAATTCAAACTTTTTTCTCTTTGGAAAATTCT13.225806451612903No Hit
TGGAGGAGGAGCCGGGTCTTATCCGGCTCCGAATCCAGAGTTCTCTTCAAC13.225806451612903No Hit
GCGTAGATTGACTGAACGCAGAAAGCCTTCCACTGGCAGGCGATCGTGTTT13.225806451612903No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT13.225806451612903No Hit
GTTTAGTAGCAATCCTGCAGCGATTGGTGCGATTACAACCCTGTCTCTTAT13.225806451612903No Hit
CAACCGATAGGTACATCGGCTCAGACGGACAAAGACTACAAGGAGCCACCA13.225806451612903No Hit
GGATGGAGACATCAGTGTGTTCCAAGAGAGAGTGAGTGGAGAGGACACTGT13.225806451612903No Hit
CTAGTGTCTATGTGTTGAAGACGGACTCTTTGGTCCTGTTTCCATGTCTTC13.225806451612903No Hit
CCCCTGTCCCGCTCCAGCTAGAGTACGAGTTCAGCTGCAGCAGCACCCCAC13.225806451612903No Hit
GGAAAAGAGAGATGAAGATCTCCTTGTCCTACCCACTGATGCTGCCATCTT13.225806451612903No Hit
TCCATGCACTTAACCTTGCCAGGGAGGAGTAACTCGTCTGTCTCTTATACA13.225806451612903No Hit
TCCTTAGCCTTCAACGCCTGTAACTTGCAGTGATTGTAATACCTGTCTCTT13.225806451612903No Hit
TCTTTTACCTTCTCCTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACA13.225806451612903No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)