##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_84.3410000000a4ba.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.714285714285715 0.0 0.0 0.0 0.0 0.0 2 36.42857142857143 0.0 0.0 0.0 0.0 0.0 3 37.142857142857146 0.0 0.0 0.0 0.0 0.0 4 35.42857142857143 0.0 0.0 0.0 0.0 0.0 5 38.0 0.0 0.0 0.0 0.0 0.0 6 37.57142857142857 0.0 0.0 0.0 0.0 0.0 7 35.0 0.0 0.0 0.0 0.0 0.0 8 33.42857142857143 0.0 0.0 0.0 0.0 0.0 9 37.57142857142857 0.0 0.0 0.0 0.0 0.0 10 34.0 0.0 0.0 0.0 0.0 0.0 11 34.0 0.0 0.0 0.0 0.0 0.0 12 39.142857142857146 0.0 0.0 0.0 0.0 0.0 13 32.57142857142857 0.0 0.0 0.0 0.0 0.0 14 35.285714285714285 0.0 0.0 0.0 0.0 0.0 15 38.57142857142857 0.0 0.0 0.0 0.0 0.0 16 39.42857142857143 0.0 0.0 0.0 0.0 0.0 17 39.42857142857143 0.0 0.0 0.0 0.0 0.0 18 39.42857142857143 0.0 0.0 0.0 0.0 0.0 19 39.714285714285715 0.0 0.0 0.0 0.0 0.0 20 39.714285714285715 0.0 0.0 0.0 0.0 0.0 21 39.42857142857143 0.0 0.0 0.0 0.0 0.0 22 38.57142857142857 0.0 0.0 0.0 0.0 0.0 23 38.285714285714285 0.0 0.0 0.0 0.0 0.0 24 38.57142857142857 0.0 0.0 0.0 0.0 0.0 25 39.42857142857143 0.0 0.0 0.0 0.0 0.0 26 38.0 0.0 0.0 0.0 0.0 0.0 27 36.142857142857146 0.0 0.0 0.0 0.0 0.0 28 38.57142857142857 0.0 0.0 0.0 0.0 0.0 29 39.142857142857146 0.0 0.0 0.0 0.0 0.0 30 38.57142857142857 0.0 0.0 0.0 0.0 0.0 31 38.285714285714285 0.0 0.0 0.0 0.0 0.0 32 38.57142857142857 0.0 0.0 0.0 0.0 0.0 33 37.57142857142857 0.0 0.0 0.0 0.0 0.0 34 39.142857142857146 0.0 0.0 0.0 0.0 0.0 35 38.285714285714285 0.0 0.0 0.0 0.0 0.0 36 39.714285714285715 0.0 0.0 0.0 0.0 0.0 37 39.42857142857143 0.0 0.0 0.0 0.0 0.0 38 37.57142857142857 0.0 0.0 0.0 0.0 0.0 39 38.285714285714285 0.0 0.0 0.0 0.0 0.0 40 39.142857142857146 0.0 0.0 0.0 0.0 0.0 41 38.285714285714285 0.0 0.0 0.0 0.0 0.0 42 38.57142857142857 0.0 0.0 0.0 0.0 0.0 43 39.42857142857143 0.0 0.0 0.0 0.0 0.0 44 39.142857142857146 0.0 0.0 0.0 0.0 0.0 45 39.42857142857143 0.0 0.0 0.0 0.0 0.0 46 38.857142857142854 0.0 0.0 0.0 0.0 0.0 47 38.57142857142857 0.0 0.0 0.0 0.0 0.0 48 35.857142857142854 0.0 0.0 0.0 0.0 0.0 49 37.857142857142854 0.0 0.0 0.0 0.0 0.0 50 38.142857142857146 0.0 0.0 0.0 0.0 0.0 51 38.57142857142857 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 32 1.0 33 0.0 34 1.0 35 0.0 36 0.0 37 0.0 38 1.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.57142857142857 0.0 42.857142857142854 28.57142857142857 2 0.0 28.57142857142857 57.14285714285714 14.285714285714285 3 0.0 14.285714285714285 57.14285714285714 28.57142857142857 4 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 5 28.57142857142857 28.57142857142857 42.857142857142854 0.0 6 0.0 71.42857142857143 14.285714285714285 14.285714285714285 7 14.285714285714285 42.857142857142854 0.0 42.857142857142854 8 14.285714285714285 71.42857142857143 0.0 14.285714285714285 9 42.857142857142854 0.0 14.285714285714285 42.857142857142854 10 0.0 42.857142857142854 28.57142857142857 28.57142857142857 11 42.857142857142854 28.57142857142857 28.57142857142857 0.0 12 0.0 14.285714285714285 71.42857142857143 14.285714285714285 13 14.285714285714285 14.285714285714285 42.857142857142854 28.57142857142857 14 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 15 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 16 28.57142857142857 28.57142857142857 42.857142857142854 0.0 17 28.57142857142857 28.57142857142857 14.285714285714285 28.57142857142857 18 28.57142857142857 42.857142857142854 28.57142857142857 0.0 19 42.857142857142854 28.57142857142857 14.285714285714285 14.285714285714285 20 28.57142857142857 14.285714285714285 0.0 57.14285714285714 21 28.57142857142857 28.57142857142857 0.0 42.857142857142854 22 0.0 28.57142857142857 42.857142857142854 28.57142857142857 23 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 24 0.0 14.285714285714285 42.857142857142854 42.857142857142854 25 28.57142857142857 0.0 57.14285714285714 14.285714285714285 26 0.0 28.57142857142857 14.285714285714285 57.14285714285714 27 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 28 14.285714285714285 42.857142857142854 42.857142857142854 0.0 29 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 30 0.0 14.285714285714285 14.285714285714285 71.42857142857143 31 28.57142857142857 57.14285714285714 14.285714285714285 0.0 32 14.285714285714285 14.285714285714285 57.14285714285714 14.285714285714285 33 14.285714285714285 28.57142857142857 14.285714285714285 42.857142857142854 34 57.14285714285714 42.857142857142854 0.0 0.0 35 28.57142857142857 57.14285714285714 14.285714285714285 0.0 36 28.57142857142857 28.57142857142857 14.285714285714285 28.57142857142857 37 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 38 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 39 0.0 28.57142857142857 57.14285714285714 14.285714285714285 40 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 41 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 42 42.857142857142854 57.14285714285714 0.0 0.0 43 57.14285714285714 0.0 28.57142857142857 14.285714285714285 44 0.0 42.857142857142854 28.57142857142857 28.57142857142857 45 0.0 28.57142857142857 28.57142857142857 42.857142857142854 46 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 47 14.285714285714285 14.285714285714285 71.42857142857143 0.0 48 28.57142857142857 14.285714285714285 28.57142857142857 28.57142857142857 49 14.285714285714285 28.57142857142857 14.285714285714285 42.857142857142854 50 28.57142857142857 28.57142857142857 14.285714285714285 28.57142857142857 51 42.857142857142854 14.285714285714285 14.285714285714285 28.57142857142857 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 1.0 31 2.0 32 1.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.5 39 1.0 40 0.5 41 0.0 42 0.5 43 1.0 44 1.5 45 2.0 46 1.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 7.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCAATCACTTCAAATCTCACCATCCCACCATCAACCAAAAGTTCATCCCC 1 14.285714285714285 No Hit TATCTAGCTAATCCGTAAGCGTTCTCATGAATGGAATGCTCAGATGGCTCG 1 14.285714285714285 No Hit TTTCTAAACAATCTCATAACCATCTATATTGTCAAGTTTGTGGTCAAGCTT 1 14.285714285714285 No Hit CTATGCAACAGTTGTGGGACCCCTGCAGGCATTGTTACATAATAACTTAAG 1 14.285714285714285 No Hit GCCTAACGGCGTGCCTCGGCATCAGCGTGCTCCGGGCGTCGGCCTGTGGGC 1 14.285714285714285 No Hit TTTGGAAAGTTATACAGAGGGACTTACAACGGAGAAGATGTAGCCATTAAG 1 14.285714285714285 No Hit GATATACAGCGTTTTGATTGATGCTTATTCAAAAGCCATTAAGAATTACGA 1 14.285714285714285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE