FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_83.3410000000a4ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_83.3410000000a4ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTTCATTTGCTAAATTCTCAACAATGGAAACACGCAATTTGGTGAAA18.333333333333332No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC18.333333333333332No Hit
ACTAAATCTCAAACATAAACCTTAAACTTGGAGTTGACCATCAGTCGCTAT18.333333333333332No Hit
CATGTATACATATGTAACAAACCTGCACGTTGTGCACATGTACCCCTGTCT18.333333333333332No Hit
TGCTACTGTTGCTCAGAATACTGAGAAAGCTAAAGATGCAGTAAAAGTAAA18.333333333333332No Hit
GCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCG18.333333333333332No Hit
CAGAGGGAGAGAAGTATAAGGAAAATTTCCTGAGGAAGTTTGTTCGCAACA18.333333333333332No Hit
GCTTTTTGTGAGCTTTCCCTAGTTTTCGTTCACTTACGTCCTTCTTCTGTC18.333333333333332No Hit
ACTTCATAGTGTCTTAACTAGTCTTCTTGATTTTGTTGTCATTGTCCTGTC18.333333333333332No Hit
GTACAAGCTTGTTCATATGCTTCACTTGCCTGGTGATAAAACTTGGCTAAA18.333333333333332No Hit
GCATATGAATTTTTGGCTACAGAGTCTTATTATGGTAGTTTATAGTAGCTG18.333333333333332No Hit
GTAGTGCTCTCTTTTCTCATCTGATTACCTTCTTCCATCAGACTCAGATGC18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)