##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_81.3410000000a484.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.714285714285715 0.0 0.0 0.0 0.0 0.0 2 35.857142857142854 0.0 0.0 0.0 0.0 0.0 3 36.285714285714285 0.0 0.0 0.0 0.0 0.0 4 36.285714285714285 0.0 0.0 0.0 0.0 0.0 5 36.714285714285715 0.0 0.0 0.0 0.0 0.0 6 39.42857142857143 0.0 0.0 0.0 0.0 0.0 7 39.42857142857143 0.0 0.0 0.0 0.0 0.0 8 39.57142857142857 0.0 0.0 0.0 0.0 0.0 9 39.285714285714285 0.0 0.0 0.0 0.0 0.0 10 36.0 0.0 0.0 0.0 0.0 0.0 11 37.92857142857143 0.0 0.0 0.0 0.0 0.0 12 38.357142857142854 0.0 0.0 0.0 0.0 0.0 13 38.07142857142857 0.0 0.0 0.0 0.0 0.0 14 36.714285714285715 0.0 0.0 0.0 0.0 0.0 15 37.785714285714285 0.0 0.0 0.0 0.0 0.0 16 38.714285714285715 0.0 0.0 0.0 0.0 0.0 17 38.57142857142857 0.0 0.0 0.0 0.0 0.0 18 36.785714285714285 0.0 0.0 0.0 0.0 0.0 19 39.0 0.0 0.0 0.0 0.0 0.0 20 37.785714285714285 0.0 0.0 0.0 0.0 0.0 21 37.42857142857143 0.0 0.0 0.0 0.0 0.0 22 38.285714285714285 0.0 0.0 0.0 0.0 0.0 23 36.785714285714285 0.0 0.0 0.0 0.0 0.0 24 38.214285714285715 0.0 0.0 0.0 0.0 0.0 25 38.642857142857146 0.0 0.0 0.0 0.0 0.0 26 38.214285714285715 0.0 0.0 0.0 0.0 0.0 27 37.5 0.0 0.0 0.0 0.0 0.0 28 36.785714285714285 0.0 0.0 0.0 0.0 0.0 29 38.357142857142854 0.0 0.0 0.0 0.0 0.0 30 39.142857142857146 0.0 0.0 0.0 0.0 0.0 31 38.357142857142854 0.0 0.0 0.0 0.0 0.0 32 39.42857142857143 0.0 0.0 0.0 0.0 0.0 33 38.642857142857146 0.0 0.0 0.0 0.0 0.0 34 38.214285714285715 0.0 0.0 0.0 0.0 0.0 35 39.0 0.0 0.0 0.0 0.0 0.0 36 39.142857142857146 0.0 0.0 0.0 0.0 0.0 37 39.0 0.0 0.0 0.0 0.0 0.0 38 39.285714285714285 0.0 0.0 0.0 0.0 0.0 39 39.285714285714285 0.0 0.0 0.0 0.0 0.0 40 38.714285714285715 0.0 0.0 0.0 0.0 0.0 41 37.357142857142854 0.0 0.0 0.0 0.0 0.0 42 39.0 0.0 0.0 0.0 0.0 0.0 43 35.0 0.0 0.0 0.0 0.0 0.0 44 37.42857142857143 0.0 0.0 0.0 0.0 0.0 45 38.57142857142857 0.0 0.0 0.0 0.0 0.0 46 39.285714285714285 0.0 0.0 0.0 0.0 0.0 47 38.285714285714285 0.0 0.0 0.0 0.0 0.0 48 39.0 0.0 0.0 0.0 0.0 0.0 49 39.57142857142857 0.0 0.0 0.0 0.0 0.0 50 38.714285714285715 0.0 0.0 0.0 0.0 0.0 51 38.714285714285715 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2116 1 0.0 2116 2 0.0 2116 3 0.0 2116 4 0.0 2116 5 0.0 2116 6 0.0 2116 7 0.0 2116 8 0.0 2116 9 0.0 2116 10 0.0 2116 11 0.0 2116 12 0.0 2116 13 0.0 2116 14 0.0 2116 15 0.0 2116 16 0.0 2116 17 0.0 2116 18 0.0 2116 19 0.0 2116 20 0.0 2116 21 0.0 2116 22 0.0 2116 23 0.0 2116 24 0.0 2116 25 0.0 2116 26 0.0 2116 27 0.0 2116 28 0.0 2116 29 0.0 2116 30 0.0 2116 31 0.0 2116 32 0.0 2116 33 0.0 2116 34 0.0 2116 35 0.0 2116 36 0.0 2116 37 0.0 2116 38 0.0 2116 39 0.0 2116 40 0.0 2116 41 0.0 2116 42 0.0 2116 43 0.0 2116 44 0.0 2116 45 0.0 2116 46 0.0 2116 47 0.0 2116 48 0.0 2116 49 0.0 2116 50 0.0 2116 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 32 1.0 33 0.0 34 1.0 35 0.0 36 1.0 37 1.0 38 3.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.714285714285715 7.142857142857142 0.0 57.14285714285714 2 7.142857142857142 14.285714285714285 71.42857142857143 7.142857142857142 3 28.57142857142857 21.428571428571427 21.428571428571427 28.57142857142857 4 0.0 21.428571428571427 42.857142857142854 35.714285714285715 5 14.285714285714285 21.428571428571427 50.0 14.285714285714285 6 28.57142857142857 64.28571428571429 7.142857142857142 0.0 7 21.428571428571427 14.285714285714285 50.0 14.285714285714285 8 14.285714285714285 50.0 14.285714285714285 21.428571428571427 9 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 10 14.285714285714285 42.857142857142854 28.57142857142857 14.285714285714285 11 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 12 21.428571428571427 35.714285714285715 28.57142857142857 14.285714285714285 13 14.285714285714285 21.428571428571427 42.857142857142854 21.428571428571427 14 28.57142857142857 7.142857142857142 35.714285714285715 28.57142857142857 15 14.285714285714285 28.57142857142857 35.714285714285715 21.428571428571427 16 28.57142857142857 14.285714285714285 14.285714285714285 42.857142857142854 17 14.285714285714285 42.857142857142854 21.428571428571427 21.428571428571427 18 28.57142857142857 64.28571428571429 0.0 7.142857142857142 19 21.428571428571427 50.0 7.142857142857142 21.428571428571427 20 28.57142857142857 21.428571428571427 14.285714285714285 35.714285714285715 21 28.57142857142857 14.285714285714285 35.714285714285715 21.428571428571427 22 0.0 50.0 14.285714285714285 35.714285714285715 23 7.142857142857142 21.428571428571427 50.0 21.428571428571427 24 14.285714285714285 28.57142857142857 21.428571428571427 35.714285714285715 25 7.142857142857142 28.57142857142857 42.857142857142854 21.428571428571427 26 21.428571428571427 21.428571428571427 42.857142857142854 14.285714285714285 27 21.428571428571427 42.857142857142854 21.428571428571427 14.285714285714285 28 7.142857142857142 42.857142857142854 21.428571428571427 28.57142857142857 29 35.714285714285715 21.428571428571427 21.428571428571427 21.428571428571427 30 35.714285714285715 35.714285714285715 14.285714285714285 14.285714285714285 31 14.285714285714285 14.285714285714285 21.428571428571427 50.0 32 28.57142857142857 21.428571428571427 28.57142857142857 21.428571428571427 33 21.428571428571427 14.285714285714285 35.714285714285715 28.57142857142857 34 14.285714285714285 14.285714285714285 42.857142857142854 28.57142857142857 35 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 36 14.285714285714285 28.57142857142857 50.0 7.142857142857142 37 21.428571428571427 42.857142857142854 28.57142857142857 7.142857142857142 38 28.57142857142857 7.142857142857142 42.857142857142854 21.428571428571427 39 14.285714285714285 42.857142857142854 7.142857142857142 35.714285714285715 40 7.142857142857142 21.428571428571427 50.0 21.428571428571427 41 21.428571428571427 21.428571428571427 35.714285714285715 21.428571428571427 42 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 43 35.714285714285715 14.285714285714285 28.57142857142857 21.428571428571427 44 21.428571428571427 28.57142857142857 14.285714285714285 35.714285714285715 45 14.285714285714285 14.285714285714285 35.714285714285715 35.714285714285715 46 14.285714285714285 42.857142857142854 21.428571428571427 21.428571428571427 47 7.142857142857142 21.428571428571427 35.714285714285715 35.714285714285715 48 35.714285714285715 14.285714285714285 28.57142857142857 21.428571428571427 49 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 50 7.142857142857142 21.428571428571427 21.428571428571427 50.0 51 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.5 31 1.0 32 0.5 33 0.0 34 0.0 35 0.0 36 1.0 37 2.0 38 1.5 39 1.0 40 1.5 41 2.0 42 2.0 43 2.0 44 1.0 45 0.0 46 0.5 47 1.0 48 1.5 49 2.0 50 1.5 51 1.0 52 0.5 53 0.0 54 1.0 55 2.0 56 1.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 14.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGCCAGCTCGGAGACGATCTCTTGTAAGGCAACGCAGAGGCTGAAATACC 1 7.142857142857142 No Hit CTCTTGAAGATGATGATGAGGATGATGATGAGGTTGGTGATTGGGAATTCG 1 7.142857142857142 No Hit CTCTTATACACATATCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG 1 7.142857142857142 No Hit GATCTGTTGTAATGTCTGATGATATTCAGATCATAAAGCAAGGCTGTGCTA 1 7.142857142857142 No Hit GTGCAATTGGTTGCTCGACAAATCTTTCAGAAGCTGTGTCATAATCCTGTC 1 7.142857142857142 No Hit GTACAGTAGACAAGGGCAAAGATCATACCACCGAAAGCCCAAGCAATGCCT 1 7.142857142857142 No Hit CTAATATAGCTCCTTCAACCATACCAACGACTCTGTCTCTTATACACATCT 1 7.142857142857142 No Hit CCTTTAGCTTATGCACCAACTCATTCTTCTCTTGCTTAATGGCTTCTCCAA 1 7.142857142857142 No Hit CTCTTACGATAATTCTTAAGCCTCTTGACATTTTCATCATTTCCCTTGGAC 1 7.142857142857142 No Hit GGATAATATTGTCCAGACGAGCGCCAAATCCTTCCTTCAAGTGACACAACC 1 7.142857142857142 No Hit ATGTTAGCTAGTCTTTAAGTTAAATGCTTGGGTATTATGTCTGTCTCTTAT 1 7.142857142857142 No Hit CTCAGGAAGGAATTCAAAAGTATTACGAAGCCCTTAAGATTGACCCACACT 1 7.142857142857142 No Hit CTGCGTTGAAGGTGAGAAGCTTCATATTGTTGTCATGTCCTCTCGTGGTGC 1 7.142857142857142 No Hit CTTACACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 1 7.142857142857142 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 7.142857142857143 34 0.0 0.0 7.142857142857143 35 0.0 0.0 7.142857142857143 36 0.0 0.0 7.142857142857143 37 0.0 0.0 7.142857142857143 38 0.0 0.0 7.142857142857143 39 0.0 0.0 7.142857142857143 >>END_MODULE >>Kmer Content pass >>END_MODULE