FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_78.3410000000a45e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_78.3410000000a45e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATCTTTTTCATCCCAATCTGTGCCTCTGTTTAAGAACTGTCTCTTA17.6923076923076925No Hit
GAGTATGATGGAGGATGAAATGCCGTTCTCCACTTTAAGACGCTTGATTAC17.6923076923076925No Hit
CACTAGCTCAAGAAGAGAGAAAACACAACAACGACGAAAACAATGAGTAAC17.6923076923076925No Hit
CTTCTATTCTCTCACTCCTTAATGCCTGTGTCTTCCTTTCCCGTACTCTGC17.6923076923076925No Hit
GTTATAGGTAAGGCTATTAACATTCCAAATATGACCCCAGTGCTTAGAATC17.6923076923076925No Hit
CTGATGGATCGTCTTCTTCGTCATCTGAGTTGTTGTTTGTAAGATTTAGAG17.6923076923076925No Hit
GTCTTTTTTCCTTGCAACAATTGGTTGGCCAATGGTCCCATTGTTACATAA17.6923076923076925No Hit
GTCCAGATCTGAGACTATCCATAGTCCCAGGCTCCAAATCCATGAGCACTG17.6923076923076925No Hit
GGTTCCTTCTTCATGGAAGAATGTAAGAGTTCCACCTATAGCTGTCTCTTA17.6923076923076925No Hit
GTGTTAATTGCTCTTTTTTTTCTTACGATGAAATACATTGTTCAGTTTCTT17.6923076923076925No Hit
GGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTACTGTC17.6923076923076925No Hit
CTATCACACTCATTGCATCTCTTATGATTAGTTCCCTCTCCACCAGTGTCC17.6923076923076925No Hit
ATCATAATACGACCTTGCAGCTTGTAGACTGTCTCTTATACACATCTCCGA17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)