##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_78.3410000000a45e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.76923076923077 0.0 0.0 0.0 0.0 0.0 2 37.07692307692308 0.0 0.0 0.0 0.0 0.0 3 37.53846153846154 0.0 0.0 0.0 0.0 0.0 4 37.07692307692308 0.0 0.0 0.0 0.0 0.0 5 36.15384615384615 0.0 0.0 0.0 0.0 0.0 6 39.53846153846154 0.0 0.0 0.0 0.0 0.0 7 39.38461538461539 0.0 0.0 0.0 0.0 0.0 8 39.38461538461539 0.0 0.0 0.0 0.0 0.0 9 38.76923076923077 0.0 0.0 0.0 0.0 0.0 10 38.69230769230769 0.0 0.0 0.0 0.0 0.0 11 38.30769230769231 0.0 0.0 0.0 0.0 0.0 12 38.61538461538461 0.0 0.0 0.0 0.0 0.0 13 38.23076923076923 0.0 0.0 0.0 0.0 0.0 14 38.07692307692308 0.0 0.0 0.0 0.0 0.0 15 39.38461538461539 0.0 0.0 0.0 0.0 0.0 16 38.92307692307692 0.0 0.0 0.0 0.0 0.0 17 37.30769230769231 0.0 0.0 0.0 0.0 0.0 18 38.38461538461539 0.0 0.0 0.0 0.0 0.0 19 39.07692307692308 0.0 0.0 0.0 0.0 0.0 20 38.53846153846154 0.0 0.0 0.0 0.0 0.0 21 38.69230769230769 0.0 0.0 0.0 0.0 0.0 22 38.76923076923077 0.0 0.0 0.0 0.0 0.0 23 38.92307692307692 0.0 0.0 0.0 0.0 0.0 24 39.38461538461539 0.0 0.0 0.0 0.0 0.0 25 39.23076923076923 0.0 0.0 0.0 0.0 0.0 26 39.38461538461539 0.0 0.0 0.0 0.0 0.0 27 37.46153846153846 0.0 0.0 0.0 0.0 0.0 28 39.23076923076923 0.0 0.0 0.0 0.0 0.0 29 38.76923076923077 0.0 0.0 0.0 0.0 0.0 30 39.23076923076923 0.0 0.0 0.0 0.0 0.0 31 38.61538461538461 0.0 0.0 0.0 0.0 0.0 32 39.07692307692308 0.0 0.0 0.0 0.0 0.0 33 38.61538461538461 0.0 0.0 0.0 0.0 0.0 34 39.38461538461539 0.0 0.0 0.0 0.0 0.0 35 39.53846153846154 0.0 0.0 0.0 0.0 0.0 36 38.76923076923077 0.0 0.0 0.0 0.0 0.0 37 37.0 0.0 0.0 0.0 0.0 0.0 38 38.92307692307692 0.0 0.0 0.0 0.0 0.0 39 36.69230769230769 0.0 0.0 0.0 0.0 0.0 40 38.38461538461539 0.0 0.0 0.0 0.0 0.0 41 37.61538461538461 0.0 0.0 0.0 0.0 0.0 42 36.0 0.0 0.0 0.0 0.0 0.0 43 38.30769230769231 0.0 0.0 0.0 0.0 0.0 44 38.15384615384615 0.0 0.0 0.0 0.0 0.0 45 37.61538461538461 0.0 0.0 0.0 0.0 0.0 46 38.61538461538461 0.0 0.0 0.0 0.0 0.0 47 37.15384615384615 0.0 0.0 0.0 0.0 0.0 48 36.84615384615385 0.0 0.0 0.0 0.0 0.0 49 36.0 0.0 0.0 0.0 0.0 0.0 50 37.38461538461539 0.0 0.0 0.0 0.0 0.0 51 37.61538461538461 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2110 1 0.0 2110 2 0.0 2110 3 0.0 2110 4 0.0 2110 5 0.0 2110 6 0.0 2110 7 0.0 2110 8 0.0 2110 9 0.0 2110 10 0.0 2110 11 0.0 2110 12 0.0 2110 13 0.0 2110 14 0.0 2110 15 0.0 2110 16 0.0 2110 17 0.0 2110 18 0.0 2110 19 0.0 2110 20 0.0 2110 21 0.0 2110 22 0.0 2110 23 0.0 2110 24 0.0 2110 25 0.0 2110 26 0.0 2110 27 0.0 2110 28 0.0 2110 29 0.0 2110 30 0.0 2110 31 0.0 2110 32 0.0 2110 33 0.0 2110 34 0.0 2110 35 0.0 2110 36 0.0 2110 37 0.0 2110 38 0.0 2110 39 0.0 2110 40 0.0 2110 41 0.0 2110 42 0.0 2110 43 0.0 2110 44 0.0 2110 45 0.0 2110 46 0.0 2110 47 0.0 2110 48 0.0 2110 49 0.0 2110 50 0.0 2110 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 32 1.0 33 0.0 34 0.0 35 1.0 36 1.0 37 0.0 38 3.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 61.53846153846154 7.6923076923076925 0.0 30.76923076923077 2 15.384615384615385 15.384615384615385 61.53846153846154 7.6923076923076925 3 23.076923076923077 15.384615384615385 30.76923076923077 30.76923076923077 4 7.6923076923076925 23.076923076923077 53.84615384615385 15.384615384615385 5 0.0 38.46153846153847 46.15384615384615 15.384615384615385 6 30.76923076923077 46.15384615384615 15.384615384615385 7.6923076923076925 7 23.076923076923077 30.76923076923077 30.76923076923077 15.384615384615385 8 7.6923076923076925 23.076923076923077 61.53846153846154 7.6923076923076925 9 0.0 7.6923076923076925 46.15384615384615 46.15384615384615 10 15.384615384615385 23.076923076923077 38.46153846153847 23.076923076923077 11 30.76923076923077 15.384615384615385 23.076923076923077 30.76923076923077 12 15.384615384615385 30.76923076923077 46.15384615384615 7.6923076923076925 13 30.76923076923077 15.384615384615385 23.076923076923077 30.76923076923077 14 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 15 23.076923076923077 23.076923076923077 30.76923076923077 23.076923076923077 16 7.6923076923076925 30.76923076923077 46.15384615384615 15.384615384615385 17 23.076923076923077 30.76923076923077 30.76923076923077 15.384615384615385 18 0.0 23.076923076923077 46.15384615384615 30.76923076923077 19 23.076923076923077 30.76923076923077 15.384615384615385 30.76923076923077 20 15.384615384615385 46.15384615384615 30.76923076923077 7.6923076923076925 21 0.0 38.46153846153847 38.46153846153847 23.076923076923077 22 15.384615384615385 23.076923076923077 46.15384615384615 15.384615384615385 23 23.076923076923077 23.076923076923077 38.46153846153847 15.384615384615385 24 30.76923076923077 7.6923076923076925 46.15384615384615 15.384615384615385 25 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 26 7.6923076923076925 15.384615384615385 38.46153846153847 38.46153846153847 27 46.15384615384615 23.076923076923077 23.076923076923077 7.6923076923076925 28 15.384615384615385 46.15384615384615 7.6923076923076925 30.76923076923077 29 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 30 23.076923076923077 23.076923076923077 38.46153846153847 15.384615384615385 31 23.076923076923077 30.76923076923077 23.076923076923077 23.076923076923077 32 15.384615384615385 23.076923076923077 30.76923076923077 30.76923076923077 33 23.076923076923077 15.384615384615385 38.46153846153847 23.076923076923077 34 15.384615384615385 23.076923076923077 46.15384615384615 15.384615384615385 35 23.076923076923077 7.6923076923076925 15.384615384615385 53.84615384615385 36 7.6923076923076925 15.384615384615385 38.46153846153847 38.46153846153847 37 0.0 46.15384615384615 38.46153846153847 15.384615384615385 38 0.0 46.15384615384615 30.76923076923077 23.076923076923077 39 23.076923076923077 15.384615384615385 53.84615384615385 7.6923076923076925 40 15.384615384615385 46.15384615384615 7.6923076923076925 30.76923076923077 41 7.6923076923076925 15.384615384615385 23.076923076923077 53.84615384615385 42 15.384615384615385 38.46153846153847 23.076923076923077 23.076923076923077 43 23.076923076923077 15.384615384615385 23.076923076923077 38.46153846153847 44 30.76923076923077 23.076923076923077 38.46153846153847 7.6923076923076925 45 15.384615384615385 23.076923076923077 61.53846153846154 0.0 46 23.076923076923077 30.76923076923077 15.384615384615385 30.76923076923077 47 15.384615384615385 7.6923076923076925 53.84615384615385 23.076923076923077 48 7.6923076923076925 30.76923076923077 30.76923076923077 30.76923076923077 49 15.384615384615385 15.384615384615385 46.15384615384615 23.076923076923077 50 15.384615384615385 30.76923076923077 46.15384615384615 7.6923076923076925 51 15.384615384615385 30.76923076923077 7.6923076923076925 46.15384615384615 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.5 27 1.0 28 0.5 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.5 37 1.0 38 2.5 39 4.0 40 3.5 41 3.0 42 2.0 43 1.0 44 1.0 45 1.0 46 1.0 47 1.0 48 0.5 49 0.0 50 0.5 51 1.0 52 0.5 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 13.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTGAATCTTTTTCATCCCAATCTGTGCCTCTGTTTAAGAACTGTCTCTTA 1 7.6923076923076925 No Hit GAGTATGATGGAGGATGAAATGCCGTTCTCCACTTTAAGACGCTTGATTAC 1 7.6923076923076925 No Hit CACTAGCTCAAGAAGAGAGAAAACACAACAACGACGAAAACAATGAGTAAC 1 7.6923076923076925 No Hit CTTCTATTCTCTCACTCCTTAATGCCTGTGTCTTCCTTTCCCGTACTCTGC 1 7.6923076923076925 No Hit GTTATAGGTAAGGCTATTAACATTCCAAATATGACCCCAGTGCTTAGAATC 1 7.6923076923076925 No Hit CTGATGGATCGTCTTCTTCGTCATCTGAGTTGTTGTTTGTAAGATTTAGAG 1 7.6923076923076925 No Hit GTCTTTTTTCCTTGCAACAATTGGTTGGCCAATGGTCCCATTGTTACATAA 1 7.6923076923076925 No Hit GTCCAGATCTGAGACTATCCATAGTCCCAGGCTCCAAATCCATGAGCACTG 1 7.6923076923076925 No Hit GGTTCCTTCTTCATGGAAGAATGTAAGAGTTCCACCTATAGCTGTCTCTTA 1 7.6923076923076925 No Hit GTGTTAATTGCTCTTTTTTTTCTTACGATGAAATACATTGTTCAGTTTCTT 1 7.6923076923076925 No Hit GGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTACTGTC 1 7.6923076923076925 No Hit CTATCACACTCATTGCATCTCTTATGATTAGTTCCCTCTCCACCAGTGTCC 1 7.6923076923076925 No Hit ATCATAATACGACCTTGCAGCTTGTAGACTGTCTCTTATACACATCTCCGA 1 7.6923076923076925 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 7.6923076923076925 30 0.0 0.0 7.6923076923076925 31 0.0 0.0 7.6923076923076925 32 0.0 0.0 7.6923076923076925 33 0.0 0.0 7.6923076923076925 34 0.0 0.0 7.6923076923076925 35 0.0 0.0 7.6923076923076925 36 0.0 0.0 7.6923076923076925 37 0.0 0.0 7.6923076923076925 38 0.0 0.0 7.6923076923076925 39 0.0 0.0 7.6923076923076925 >>END_MODULE >>Kmer Content pass >>END_MODULE