FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_77.3410000000a441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_77.3410000000a441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAATACTACCAATAAAATCAATATAGCACAGTAGCTGTTCAGTTTTAG19.090909090909092No Hit
TCTCCAGTACCAGTGCAAGAAAGCCTTCCTCATGAACATGGCTGTGAACAC19.090909090909092No Hit
CTAGTAGAGCACCGGAGATTCCGTAGAGATTCATGTAGAGCATCAATTTCC19.090909090909092No Hit
GTACTTTGTGTCTTGTTATGTTTTTGAGTCTATGACTCTTTCTTGCTGTCT19.090909090909092No Hit
CTTCATCTCTTCCATTGTCATCCTCATTCAAAAGCCAACTACCTGTCTCTT19.090909090909092No Hit
CAGAGTGAGGTTTCTGCAGACAATCAAGGATATAGCTAGTGCTGTCTCTTA19.090909090909092No Hit
GACATCATCGAGGCTATTGAGGATCAGAGGGATTCAGACTTTTACGCTTCC19.090909090909092No Hit
CTTCCATTAGTTTCCCCCACATATTTAACTTCCCATAATTTACTGCCCAAG19.090909090909092No Hit
GTAGAGATACCTCATAAACAGAAGATTCAAGCTAAGGCTTACCAAGATCTG19.090909090909092No Hit
GTATGTACCAGTGTCGTTGACGCAGGGAATGCAGACCAAGCTTATCAGTGA19.090909090909092No Hit
CCCTTTACCTTGGCTACTCACTCTTCATGGCTGTCTTTTATACACATCTCC19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)