##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_76.3410000000a434.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.142857142857146 0.0 0.0 0.0 0.0 0.0 2 38.0 0.0 0.0 0.0 0.0 0.0 3 38.0 0.0 0.0 0.0 0.0 0.0 4 37.142857142857146 0.0 0.0 0.0 0.0 0.0 5 37.142857142857146 0.0 0.0 0.0 0.0 0.0 6 39.42857142857143 0.0 0.0 0.0 0.0 0.0 7 39.42857142857143 0.0 0.0 0.0 0.0 0.0 8 39.714285714285715 0.0 0.0 0.0 0.0 0.0 9 39.714285714285715 0.0 0.0 0.0 0.0 0.0 10 35.57142857142857 0.0 0.0 0.0 0.0 0.0 11 38.142857142857146 0.0 0.0 0.0 0.0 0.0 12 38.857142857142854 0.0 0.0 0.0 0.0 0.0 13 38.285714285714285 0.0 0.0 0.0 0.0 0.0 14 36.142857142857146 0.0 0.0 0.0 0.0 0.0 15 38.142857142857146 0.0 0.0 0.0 0.0 0.0 16 36.142857142857146 0.0 0.0 0.0 0.0 0.0 17 38.142857142857146 0.0 0.0 0.0 0.0 0.0 18 37.857142857142854 0.0 0.0 0.0 0.0 0.0 19 36.714285714285715 0.0 0.0 0.0 0.0 0.0 20 35.285714285714285 0.0 0.0 0.0 0.0 0.0 21 34.714285714285715 0.0 0.0 0.0 0.0 0.0 22 37.0 0.0 0.0 0.0 0.0 0.0 23 35.57142857142857 0.0 0.0 0.0 0.0 0.0 24 39.142857142857146 0.0 0.0 0.0 0.0 0.0 25 35.57142857142857 0.0 0.0 0.0 0.0 0.0 26 34.42857142857143 0.0 0.0 0.0 0.0 0.0 27 35.285714285714285 0.0 0.0 0.0 0.0 0.0 28 35.857142857142854 0.0 0.0 0.0 0.0 0.0 29 35.57142857142857 0.0 0.0 0.0 0.0 0.0 30 35.857142857142854 0.0 0.0 0.0 0.0 0.0 31 37.57142857142857 0.0 0.0 0.0 0.0 0.0 32 37.57142857142857 0.0 0.0 0.0 0.0 0.0 33 38.285714285714285 0.0 0.0 0.0 0.0 0.0 34 39.42857142857143 0.0 0.0 0.0 0.0 0.0 35 35.857142857142854 0.0 0.0 0.0 0.0 0.0 36 35.857142857142854 0.0 0.0 0.0 0.0 0.0 37 37.857142857142854 0.0 0.0 0.0 0.0 0.0 38 36.714285714285715 0.0 0.0 0.0 0.0 0.0 39 35.57142857142857 0.0 0.0 0.0 0.0 0.0 40 37.57142857142857 0.0 0.0 0.0 0.0 0.0 41 39.142857142857146 0.0 0.0 0.0 0.0 0.0 42 39.142857142857146 0.0 0.0 0.0 0.0 0.0 43 35.857142857142854 0.0 0.0 0.0 0.0 0.0 44 38.857142857142854 0.0 0.0 0.0 0.0 0.0 45 36.142857142857146 0.0 0.0 0.0 0.0 0.0 46 37.57142857142857 0.0 0.0 0.0 0.0 0.0 47 35.57142857142857 0.0 0.0 0.0 0.0 0.0 48 37.857142857142854 0.0 0.0 0.0 0.0 0.0 49 37.57142857142857 0.0 0.0 0.0 0.0 0.0 50 35.857142857142854 0.0 0.0 0.0 0.0 0.0 51 37.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 2.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.57142857142857 42.857142857142854 0.0 28.57142857142857 2 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 3 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 4 0.0 28.57142857142857 57.14285714285714 14.285714285714285 5 0.0 57.14285714285714 14.285714285714285 28.57142857142857 6 42.857142857142854 28.57142857142857 0.0 28.57142857142857 7 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 8 0.0 57.14285714285714 14.285714285714285 28.57142857142857 9 0.0 42.857142857142854 0.0 57.14285714285714 10 42.857142857142854 0.0 28.57142857142857 28.57142857142857 11 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 12 28.57142857142857 57.14285714285714 0.0 14.285714285714285 13 0.0 28.57142857142857 57.14285714285714 14.285714285714285 14 0.0 42.857142857142854 57.14285714285714 0.0 15 0.0 28.57142857142857 14.285714285714285 57.14285714285714 16 28.57142857142857 14.285714285714285 57.14285714285714 0.0 17 28.57142857142857 14.285714285714285 42.857142857142854 14.285714285714285 18 28.57142857142857 14.285714285714285 57.14285714285714 0.0 19 0.0 28.57142857142857 42.857142857142854 28.57142857142857 20 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 21 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 22 42.857142857142854 14.285714285714285 28.57142857142857 14.285714285714285 23 42.857142857142854 0.0 14.285714285714285 42.857142857142854 24 14.285714285714285 57.14285714285714 0.0 28.57142857142857 25 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 26 28.57142857142857 14.285714285714285 28.57142857142857 28.57142857142857 27 14.285714285714285 57.14285714285714 28.57142857142857 0.0 28 0.0 42.857142857142854 42.857142857142854 14.285714285714285 29 0.0 28.57142857142857 42.857142857142854 28.57142857142857 30 0.0 14.285714285714285 0.0 85.71428571428571 31 0.0 14.285714285714285 57.14285714285714 28.57142857142857 32 28.57142857142857 0.0 42.857142857142854 28.57142857142857 33 0.0 28.57142857142857 42.857142857142854 28.57142857142857 34 28.57142857142857 14.285714285714285 14.285714285714285 42.857142857142854 35 0.0 42.857142857142854 57.14285714285714 0.0 36 14.285714285714285 28.57142857142857 14.285714285714285 42.857142857142854 37 0.0 42.857142857142854 14.285714285714285 42.857142857142854 38 0.0 14.285714285714285 42.857142857142854 42.857142857142854 39 42.857142857142854 28.57142857142857 28.57142857142857 0.0 40 0.0 28.57142857142857 57.14285714285714 14.285714285714285 41 28.57142857142857 14.285714285714285 14.285714285714285 42.857142857142854 42 14.285714285714285 14.285714285714285 42.857142857142854 28.57142857142857 43 0.0 28.57142857142857 14.285714285714285 57.14285714285714 44 0.0 14.285714285714285 57.14285714285714 28.57142857142857 45 14.285714285714285 28.57142857142857 57.14285714285714 0.0 46 0.0 28.57142857142857 42.857142857142854 28.57142857142857 47 14.285714285714285 28.57142857142857 28.57142857142857 28.57142857142857 48 14.285714285714285 28.57142857142857 42.857142857142854 14.285714285714285 49 0.0 42.857142857142854 0.0 57.14285714285714 50 0.0 28.57142857142857 42.857142857142854 28.57142857142857 51 14.285714285714285 42.857142857142854 14.285714285714285 28.57142857142857 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.5 35 1.0 36 1.5 37 2.0 38 1.0 39 0.0 40 0.5 41 1.0 42 1.0 43 1.0 44 1.0 45 1.0 46 0.5 47 0.0 48 0.5 49 1.0 50 0.5 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 7.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGTAGGACGAGTTCTTGTTCTGAACATTCAGCATCCTGTCTCTTTACACA 1 14.285714285714285 No Hit GTCCAGATACCATTTTCTTGAGTAGAGAAATCACAAAATACTCATCCTCAC 1 14.285714285714285 No Hit CGTTCATACTTACACATGCATGGCTTAATCTTTGAGACAAGCATATGACTA 1 14.285714285714285 No Hit CTTTCCTAATCAAAAGATAGGAGAGCACCCCTTCTCCTGTCTCTTATACAC 1 14.285714285714285 No Hit AGCAAGCCCCTCTTCTTTCAGTCGAGTTACTTCGTACCTTGACTGAAGATT 1 14.285714285714285 No Hit GCATTATACGGAATATGAACAGCATTATTCCCATATACGCTGTCTCTTATA 1 14.285714285714285 No Hit ATCAACGCAGAGTACGGTTAACCCCGTACCTGTCTCTTATACACATCTCCG 1 14.285714285714285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 14.285714285714286 31 0.0 0.0 14.285714285714286 32 0.0 0.0 14.285714285714286 33 0.0 0.0 14.285714285714286 34 0.0 0.0 14.285714285714286 35 0.0 0.0 14.285714285714286 36 0.0 0.0 14.285714285714286 37 0.0 0.0 28.571428571428573 38 0.0 0.0 28.571428571428573 39 0.0 0.0 28.571428571428573 >>END_MODULE >>Kmer Content pass >>END_MODULE