FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_74.3410000000a41a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_74.3410000000a41a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGCTATAGCATCAGAAACATCAGAATCTCCCTCAGACCCTTCTCCAG12.857142857142857No Hit
TCCATAAAGACTTCAAGCTCTATACTGGCACTAAGCCTTAGCATACAATTC12.857142857142857No Hit
GATCTGAAAAGTCTTCAAGTTCGATCCTCTTCCGCCGCCAGAGGAAGCTTT12.857142857142857No Hit
AACGTATTCAGTTCTTTGATTCATTCTGTGGCGCGAAATCCATCATTGGCT12.857142857142857No Hit
CTTTTAATACAAAGCTTTTATCACACAAATGGCATGTGGTTACTTTTATCA12.857142857142857No Hit
GTGAAAGCTGCTGCTCCAACGAAGAAGAAAACAACTTCCTCTCACCCTACC12.857142857142857No Hit
CAATAGTCTGAAGACGACGCTCAAGAAAGTTCTCAACAGTCAAAGCCAAGA12.857142857142857No Hit
CATATAGCAGGTGGTATTGATATCAAAAGTGATGAACGTCAATCAAAAGGC12.857142857142857No Hit
CTTTAAATAGGTGTTCATAATTCTTCTGGACCTCCTCGGGGCTCAGCTTGG12.857142857142857No Hit
GACTAGGAGAGGGATACCATTAAGCGACGTCTTGCTCAACAAATCATGGAG12.857142857142857No Hit
GCTCTGAGTCTGCTGCTGCAGTCATAGTGATGTGCTTTTCAGCTCAGAACT12.857142857142857No Hit
CAATGTTTCTTTGGACTTTAAAACAAACGACAACAACCTCTATGTCTGTCT12.857142857142857No Hit
CTACTCATTCTCGCCGTTCATAACTGGTCCGTCGCCGTATCTTCTCAATCC12.857142857142857No Hit
TTGTGTGTGTTTCTTGTTGCAAGACAACCATATCTATTGGTTTTGGATTGT12.857142857142857No Hit
GTGTTTACCCTTGGTCCTTATCATCAGGAACAGGTTGAAGTATGCTCTGAC12.857142857142857No Hit
AGCTTCTTGTGAGGGAAAATTACTATTCTGACAGGTAAATCTGGAATTGGA12.857142857142857No Hit
AAGAAACCATGCCGTACATGACACTCGAAGAAACCCAGTAGTACCCATCCG12.857142857142857No Hit
GGTATATTGCTTGTCTACGATGTAACGGATGAGTCATCTTTTAACAATATT12.857142857142857No Hit
CTGATGGGACCATAAGCATCAATTGCCAAACCAGTGGCAATGGTACTGAGC12.857142857142857No Hit
CTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATT12.857142857142857No Hit
GAATGAGACAGATTCTGTTTCTTCTCTTTTGCTTCATCTCTCTCTATCTGT12.857142857142857No Hit
AAGTAATTTATTTGCTACTTTAAGTACTTTGTGTCTTGTTACTGTCTCTTA12.857142857142857No Hit
GAGCAATTTGTGAAGGAGATGGAAGTGACGAGGCGAAATGGGGAGCTGGTG12.857142857142857No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATAT12.857142857142857No Hit
GCGATGACAAAACTACTTCGGTTGCAGAGGGTCAGAGTCTGTCTCTTATAC12.857142857142857No Hit
ATCGTAGACAAAGAAGTGCAGATATTTTAGCGGAGTCGATGTTGGTTATAG12.857142857142857No Hit
CTTCTGACCCTTTCCAAACACTGCTTTGATATCTGCTGTTCCCATATTCGT12.857142857142857No Hit
ATCTAAAACTGTGCTCTACAATCTCATAACAAATCTATTACTGCTGTCTCT12.857142857142857No Hit
GTTCTTCAGTAAACATATCACCATTTGATGATTCAACATCTAAAATGAGAT12.857142857142857No Hit
TGCCCGCACTCCATACAACTAACAATGCTCGACGTTTCGCCTCCAAATACA12.857142857142857No Hit
CCTTAAACTCCTCAAGTGCCTTCTTCTGAACTTCCTCAGCAGTTAAAACAA12.857142857142857No Hit
GAGTAATGAAAGCTTCTCACTTGTTTCATGTTTCTATGCTTACTAGGAAAA12.857142857142857No Hit
AAGAAAATCCAAGACAAAAACATTGTACCATAATGGGAGTTTCAAAGAAAT12.857142857142857No Hit
GTTTATAGAAGCTTTGAGCGTGGTTCCTGCCTGGCACAGAGGTCGTTCGAC12.857142857142857No Hit
ACTATGCACACAAGTTAAAAAGAAAGTGTTACAATTTTCAAATTAGACACA12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)