##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l01n01 kb2_2_73.3410000000a3ff.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.142857142857146 0.0 0.0 0.0 0.0 0.0 2 37.57142857142857 0.0 0.0 0.0 0.0 0.0 3 37.57142857142857 0.0 0.0 0.0 0.0 0.0 4 37.57142857142857 0.0 0.0 0.0 0.0 0.0 5 36.285714285714285 0.0 0.0 0.0 0.0 0.0 6 39.57142857142857 0.0 0.0 0.0 0.0 0.0 7 38.07142857142857 0.0 0.0 0.0 0.0 0.0 8 39.285714285714285 0.0 0.0 0.0 0.0 0.0 9 38.42857142857143 0.0 0.0 0.0 0.0 0.0 10 39.57142857142857 0.0 0.0 0.0 0.0 0.0 11 39.0 0.0 0.0 0.0 0.0 0.0 12 39.42857142857143 0.0 0.0 0.0 0.0 0.0 13 39.42857142857143 0.0 0.0 0.0 0.0 0.0 14 39.42857142857143 0.0 0.0 0.0 0.0 0.0 15 38.57142857142857 0.0 0.0 0.0 0.0 0.0 16 39.285714285714285 0.0 0.0 0.0 0.0 0.0 17 37.785714285714285 0.0 0.0 0.0 0.0 0.0 18 38.857142857142854 0.0 0.0 0.0 0.0 0.0 19 39.57142857142857 0.0 0.0 0.0 0.0 0.0 20 38.57142857142857 0.0 0.0 0.0 0.0 0.0 21 37.642857142857146 0.0 0.0 0.0 0.0 0.0 22 37.785714285714285 0.0 0.0 0.0 0.0 0.0 23 38.92857142857143 0.0 0.0 0.0 0.0 0.0 24 38.57142857142857 0.0 0.0 0.0 0.0 0.0 25 38.714285714285715 0.0 0.0 0.0 0.0 0.0 26 38.07142857142857 0.0 0.0 0.0 0.0 0.0 27 39.142857142857146 0.0 0.0 0.0 0.0 0.0 28 37.07142857142857 0.0 0.0 0.0 0.0 0.0 29 38.357142857142854 0.0 0.0 0.0 0.0 0.0 30 39.285714285714285 0.0 0.0 0.0 0.0 0.0 31 38.857142857142854 0.0 0.0 0.0 0.0 0.0 32 37.5 0.0 0.0 0.0 0.0 0.0 33 38.642857142857146 0.0 0.0 0.0 0.0 0.0 34 39.42857142857143 0.0 0.0 0.0 0.0 0.0 35 39.42857142857143 0.0 0.0 0.0 0.0 0.0 36 39.57142857142857 0.0 0.0 0.0 0.0 0.0 37 38.857142857142854 0.0 0.0 0.0 0.0 0.0 38 39.57142857142857 0.0 0.0 0.0 0.0 0.0 39 39.285714285714285 0.0 0.0 0.0 0.0 0.0 40 39.57142857142857 0.0 0.0 0.0 0.0 0.0 41 37.07142857142857 0.0 0.0 0.0 0.0 0.0 42 35.714285714285715 0.0 0.0 0.0 0.0 0.0 43 36.857142857142854 0.0 0.0 0.0 0.0 0.0 44 37.07142857142857 0.0 0.0 0.0 0.0 0.0 45 38.642857142857146 0.0 0.0 0.0 0.0 0.0 46 39.0 0.0 0.0 0.0 0.0 0.0 47 39.42857142857143 0.0 0.0 0.0 0.0 0.0 48 38.57142857142857 0.0 0.0 0.0 0.0 0.0 49 36.785714285714285 0.0 0.0 0.0 0.0 0.0 50 38.714285714285715 0.0 0.0 0.0 0.0 0.0 51 33.214285714285715 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2115 1 0.0 2115 2 0.0 2115 3 0.0 2115 4 0.0 2115 5 0.0 2115 6 0.0 2115 7 0.0 2115 8 0.0 2115 9 0.0 2115 10 0.0 2115 11 0.0 2115 12 0.0 2115 13 0.0 2115 14 0.0 2115 15 0.0 2115 16 0.0 2115 17 0.0 2115 18 0.0 2115 19 0.0 2115 20 0.0 2115 21 0.0 2115 22 0.0 2115 23 0.0 2115 24 0.0 2115 25 0.0 2115 26 0.0 2115 27 0.0 2115 28 0.0 2115 29 0.0 2115 30 0.0 2115 31 0.0 2115 32 0.0 2115 33 0.0 2115 34 0.0 2115 35 0.0 2115 36 0.0 2115 37 0.0 2115 38 0.0 2115 39 0.0 2115 40 0.0 2115 41 0.0 2115 42 0.0 2115 43 0.0 2115 44 0.0 2115 45 0.0 2115 46 0.0 2115 47 0.0 2115 48 0.0 2115 49 0.0 2115 50 0.0 2115 51 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 31 1.0 32 0.0 33 0.0 34 0.0 35 0.0 36 1.0 37 1.0 38 2.0 39 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.857142857142854 14.285714285714285 7.142857142857142 35.714285714285715 2 14.285714285714285 21.428571428571427 50.0 14.285714285714285 3 35.714285714285715 42.857142857142854 14.285714285714285 7.142857142857142 4 7.142857142857142 21.428571428571427 21.428571428571427 50.0 5 0.0 71.42857142857143 14.285714285714285 14.285714285714285 6 28.57142857142857 64.28571428571429 7.142857142857142 0.0 7 14.285714285714285 28.57142857142857 21.428571428571427 35.714285714285715 8 14.285714285714285 57.14285714285714 21.428571428571427 7.142857142857142 9 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 10 14.285714285714285 28.57142857142857 14.285714285714285 42.857142857142854 11 14.285714285714285 35.714285714285715 7.142857142857142 42.857142857142854 12 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 13 35.714285714285715 21.428571428571427 28.57142857142857 14.285714285714285 14 21.428571428571427 7.142857142857142 35.714285714285715 35.714285714285715 15 28.57142857142857 21.428571428571427 14.285714285714285 35.714285714285715 16 14.285714285714285 21.428571428571427 50.0 14.285714285714285 17 7.142857142857142 35.714285714285715 35.714285714285715 21.428571428571427 18 14.285714285714285 28.57142857142857 50.0 7.142857142857142 19 14.285714285714285 35.714285714285715 35.714285714285715 14.285714285714285 20 7.142857142857142 57.14285714285714 21.428571428571427 14.285714285714285 21 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 22 21.428571428571427 21.428571428571427 35.714285714285715 21.428571428571427 23 14.285714285714285 35.714285714285715 21.428571428571427 28.57142857142857 24 35.714285714285715 35.714285714285715 21.428571428571427 7.142857142857142 25 28.57142857142857 21.428571428571427 28.57142857142857 21.428571428571427 26 21.428571428571427 28.57142857142857 21.428571428571427 28.57142857142857 27 42.857142857142854 21.428571428571427 35.714285714285715 0.0 28 21.428571428571427 14.285714285714285 42.857142857142854 21.428571428571427 29 14.285714285714285 35.714285714285715 42.857142857142854 7.142857142857142 30 57.14285714285714 14.285714285714285 14.285714285714285 14.285714285714285 31 21.428571428571427 35.714285714285715 28.57142857142857 14.285714285714285 32 0.0 35.714285714285715 50.0 14.285714285714285 33 21.428571428571427 28.57142857142857 14.285714285714285 35.714285714285715 34 35.714285714285715 35.714285714285715 14.285714285714285 14.285714285714285 35 28.57142857142857 35.714285714285715 21.428571428571427 14.285714285714285 36 35.714285714285715 14.285714285714285 14.285714285714285 35.714285714285715 37 21.428571428571427 21.428571428571427 28.57142857142857 28.57142857142857 38 14.285714285714285 42.857142857142854 35.714285714285715 7.142857142857142 39 21.428571428571427 21.428571428571427 21.428571428571427 35.714285714285715 40 28.57142857142857 21.428571428571427 35.714285714285715 14.285714285714285 41 14.285714285714285 14.285714285714285 28.57142857142857 42.857142857142854 42 28.57142857142857 21.428571428571427 50.0 0.0 43 28.57142857142857 35.714285714285715 7.142857142857142 28.57142857142857 44 21.428571428571427 57.14285714285714 7.142857142857142 14.285714285714285 45 7.142857142857142 50.0 28.57142857142857 14.285714285714285 46 14.285714285714285 28.57142857142857 50.0 7.142857142857142 47 28.57142857142857 28.57142857142857 28.57142857142857 14.285714285714285 48 35.714285714285715 35.714285714285715 21.428571428571427 7.142857142857142 49 28.57142857142857 42.857142857142854 14.285714285714285 14.285714285714285 50 14.285714285714285 42.857142857142854 21.428571428571427 21.428571428571427 51 28.57142857142857 14.285714285714285 35.714285714285715 21.428571428571427 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.5 29 1.0 30 1.0 31 1.0 32 0.5 33 0.0 34 1.5 35 3.0 36 2.0 37 1.0 38 1.0 39 1.0 40 0.5 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.5 53 1.0 54 1.5 55 2.0 56 1.5 57 1.0 58 1.0 59 1.0 60 1.0 61 1.0 62 0.5 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 14.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGATACGTGGCGAGGTTAACCCGTGTGGGGAAGCCGTAGCGAAAGCGAGT 1 7.142857142857142 No Hit CTTCAAAATCAAGCCTATCTAGAGAAGAAAATCAGTTTGTGACATGTCAAG 1 7.142857142857142 No Hit GTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGCTGTCTCTTATAC 1 7.142857142857142 No Hit GGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTCACACCTTGGGCG 1 7.142857142857142 No Hit GTGAAAATTCCGGCGGAGGATTCGGAGTGGATGGAGGAGGCGGATTCGGAT 1 7.142857142857142 No Hit ATATAAGAGAAGTTTCAAAACTGAGCAGAGTACCAAAAGAAACGAAGTGAG 1 7.142857142857142 No Hit CTACTATCACCGGCATCTCCGTCACCGTCGGCTATCTCTCTGGGATTAAGC 1 7.142857142857142 No Hit CATCAACAGCTTTCCTCTTAGTTGCAGCAGCAGTGGCACGAGGAACGGGCT 1 7.142857142857142 No Hit TACCAAGACCAAGTGATAGATATACCTTTGTTCCTGCATCCTACCTTTCTT 1 7.142857142857142 No Hit GGAGATCAACCATGCTGGTGCCGGTGGATTATGGGCCGAACTTGTTAGCAA 1 7.142857142857142 No Hit CAATAATAATACATCAAATAAGCTTCTCTCCTAAGCCTCTCTAAATATATC 1 7.142857142857142 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAA 1 7.142857142857142 No Hit CCACCACACACACCATTAAACCAAATGCAAAAAAAAAAAGTTAAGAAAACT 1 7.142857142857142 No Hit GTGTAGTTCTGAATACTTAAAAACGATTTGGTCGCCGACTTTGAAAGATTG 1 7.142857142857142 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.0 29 0.0 0.0 0.0 30 0.0 0.0 0.0 31 0.0 0.0 0.0 32 0.0 0.0 0.0 33 0.0 0.0 0.0 34 0.0 0.0 0.0 35 0.0 0.0 0.0 36 0.0 0.0 0.0 37 0.0 0.0 0.0 38 0.0 0.0 0.0 39 0.0 0.0 7.142857142857143 >>END_MODULE >>Kmer Content pass >>END_MODULE