FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_71.3410000000a3d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_71.3410000000a3d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC26420.52877138413686No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT241.8662519440124419No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC50.38880248833592534No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG30.23328149300155523No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATTATATCTCGTATGCCGTC20.15552099533437014No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAA20.15552099533437014No Hit
CTTATACACTCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT20.15552099533437014Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCAGTC20.15552099533437014No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCCATATCTCGTATGCCGTC20.15552099533437014No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC401.4042598E-945.045
AGCCCAC454.0017767E-940.017
GTATGCC454.0017767E-940.042
ACGCTAT454.0017767E-940.030
ATCTCGT454.0017767E-940.037
GCCCACG454.0017767E-940.018
CCACGAG454.0017767E-940.020
TATGCCG454.0017767E-940.043
GAGCCCA454.0017767E-940.016
CACGAGA454.0017767E-940.021
CCCACGA454.0017767E-940.019
CGCTATA454.0017767E-940.031
TCTCGTA454.0017767E-940.038
GACGCTA454.0017767E-940.029
ACGAGAC454.0017767E-940.022
AGACGAC454.0017767E-940.025
ATGCCGT454.0017767E-940.044
CGTATGC454.0017767E-940.041
GACGACG454.0017767E-940.026
CGAGCCC454.0017767E-940.015