FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_69.3410000000a3bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_69.3410000000a3bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2052661
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC261321.2730791884290684No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT33330.16237459570771792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29800.042.8106145
ATGCCGT32500.039.18480744
GCCCACG33650.038.11125618
GACGCTA33650.038.0443929
CGCTATA33600.038.03404631
TATGCCG33550.038.0255243
CGACGCT33750.037.99833328
ACGACGC34050.037.66354827
CCCACGA34200.037.6299319
GTATGCC34600.036.93479542
CGAGACG34900.036.81070723
CGTATGC34900.036.61730641
ACGCTAT34950.036.5649230
CGAGCCC35050.036.52478815
AGCCCAC35350.036.40575817
CCACGAG35500.036.25193420
CTCGTAT35350.036.21481739
TATCTCG35450.035.92226436
ACGAGAC35800.035.88529622
CCGAGCC35700.035.8597714
Produced by FastQC (version 0.11.2)