FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_64.3410000000a36b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_64.3410000000a36b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC19118.0188679245283No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT90.8490566037735849No Hit
CTTATACACACTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT50.4716981132075472Illumina PCR Primer Index 11 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG30.2830188679245283No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGACGTC30.2830188679245283No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC30.2830188679245283No Hit
CTTCAGTATCGAATCTGAGGTTCCGAAACTGATTGCTTGGGTTAAGAAGCT20.18867924528301888No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCATC20.18867924528301888No Hit
CTTATACACATCTCCGGCCCACGAGACGACGCTATATCTCGTATGCCGTCT20.18867924528301888Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT20.18867924528301888Illumina PCR Primer Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG150.00953857644.99999610
CGAGCCC150.00953857644.99999615
CCGAGCC150.00953857644.99999614
TCTCCGA150.00953857644.99999611
TCCGAGC150.00953857644.99999613
CTCCGAG150.00953857644.99999612
Produced by FastQC (version 0.11.2)