FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_62.3410000000a342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_62.3410000000a342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC24917.887931034482758No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT211.5086206896551724No Hit
CTTATACAATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT30.21551724137931033Illumina PCR Primer Index 11 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG30.21551724137931033No Hit
ACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAA20.14367816091954022No Hit
CTCATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC20.14367816091954022No Hit
CTTATACACATCTCCGAGCCCACGAGAGACGCTATATCTCGTATGCCGTCT20.14367816091954022Illumina PCR Primer Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC456.184564E-1145.045
CATCTCC601.6370905E-1137.4999969
ACATCTC601.6370905E-1137.4999968
ATCTCCG554.4383341E-1036.81818410
TCTCCGA554.4383341E-1036.81818411
CACATCT653.8198777E-1134.6153837
GTATGCC601.0477379E-933.7542
CTATATC601.0477379E-933.7533
ACGCTAT601.0477379E-933.7530
ATCTCGT601.0477379E-933.7537
TATATCT601.0477379E-933.7534
CCACGAG601.0477379E-933.7520
TATGCCG601.0477379E-933.7543
CACGAGA601.0477379E-933.7521
CCCACGA601.0477379E-933.7519
CGCTATA601.0477379E-933.7531
GCTATAT601.0477379E-933.7532
TCTCGTA601.0477379E-933.7538
GACGCTA601.0477379E-933.7529
TATCTCG601.0477379E-933.7536