Basic Statistics
Measure | Value |
---|---|
Filename | H23V3BCXX l01n01 kb2_2_62.3410000000a342.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1392 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC | 249 | 17.887931034482758 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT | 21 | 1.5086206896551724 | No Hit |
CTTATACAATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT | 3 | 0.21551724137931033 | Illumina PCR Primer Index 11 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG | 3 | 0.21551724137931033 | No Hit |
ACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAA | 2 | 0.14367816091954022 | No Hit |
CTCATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC | 2 | 0.14367816091954022 | No Hit |
CTTATACACATCTCCGAGCCCACGAGAGACGCTATATCTCGTATGCCGTCT | 2 | 0.14367816091954022 | Illumina PCR Primer Index 11 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 45 | 6.184564E-11 | 45.0 | 45 |
CATCTCC | 60 | 1.6370905E-11 | 37.499996 | 9 |
ACATCTC | 60 | 1.6370905E-11 | 37.499996 | 8 |
ATCTCCG | 55 | 4.4383341E-10 | 36.818184 | 10 |
TCTCCGA | 55 | 4.4383341E-10 | 36.818184 | 11 |
CACATCT | 65 | 3.8198777E-11 | 34.615383 | 7 |
GTATGCC | 60 | 1.0477379E-9 | 33.75 | 42 |
CTATATC | 60 | 1.0477379E-9 | 33.75 | 33 |
ACGCTAT | 60 | 1.0477379E-9 | 33.75 | 30 |
ATCTCGT | 60 | 1.0477379E-9 | 33.75 | 37 |
TATATCT | 60 | 1.0477379E-9 | 33.75 | 34 |
CCACGAG | 60 | 1.0477379E-9 | 33.75 | 20 |
TATGCCG | 60 | 1.0477379E-9 | 33.75 | 43 |
CACGAGA | 60 | 1.0477379E-9 | 33.75 | 21 |
CCCACGA | 60 | 1.0477379E-9 | 33.75 | 19 |
CGCTATA | 60 | 1.0477379E-9 | 33.75 | 31 |
GCTATAT | 60 | 1.0477379E-9 | 33.75 | 32 |
TCTCGTA | 60 | 1.0477379E-9 | 33.75 | 38 |
GACGCTA | 60 | 1.0477379E-9 | 33.75 | 29 |
TATCTCG | 60 | 1.0477379E-9 | 33.75 | 36 |