FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l01n01 kb2_2_6.34100000009f7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l01n01 kb2_2_6.34100000009f7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21.9047619047619049TruSeq Adapter, Index 12 (95% over 21bp)
ACTCAGAAGACGAGGAGGATATTTTAGCCCGAGTGGATCTAGAAACTACCA10.9523809523809524No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTA10.9523809523809524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGTTT10.9523809523809524No Hit
GTCAAATGATGATGATATGTTTCTTGCTCGTCTTGTTCATCATCCTATTCC10.9523809523809524No Hit
CCAAGAAGGTTGTGAAGAAGAGGTCTGCTAAGTTCATCAGACCCCAGAGTG10.9523809523809524No Hit
GTTGCGTCCAATATCCCTTTTAGAATGGCCTAGTGTGGCGGCCTTTAGCCT10.9523809523809524No Hit
CATTTCGGGGAGAACTAGCTATCTCCAGGTTCGGTTAGCTTTTCACTCCTA10.9523809523809524No Hit
GACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACTAATTAG10.9523809523809524No Hit
ATCCAGATCAGTCCGTGATGGTTCTGGAAGCTGGCCGCATGGATTCCATCT10.9523809523809524No Hit
CAATACACCGATAGATCTTCAAGAACAGGACAAATAGATAGAAGCTCCTGA10.9523809523809524No Hit
ATCCAAAGTCGAGAGGGAAACAGCCCAGATCGTACGCTAAGGTCCCTAAGC10.9523809523809524No Hit
GCTCTATCCCCAGCACGACAAAGTTTCACAAGATTACCCAGTCCTTTCGGA10.9523809523809524No Hit
TCAATGTACACATCACAGAATTGGTATTGCCACCATCTGTCTCTTATACAC10.9523809523809524No Hit
CACTGAGTGACTACTATGTGCAAAGCACTATGCTAGGTCTGTCTCTTATAC10.9523809523809524No Hit
CTTCAAGCATCCGAAATACGCCGGGGTTGTAAATGACATAGCGTTCCTGAT10.9523809523809524No Hit
CCCTACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG10.9523809523809524No Hit
TGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGATGCGTCCTGG10.9523809523809524No Hit
CTCGTCGTAGTTTGGCGACCTTCAGTAGGGGCCTTTAGTCTTTTGATTAGA10.9523809523809524No Hit
GTGTACATCAGATTATGTTGGATCACGTTCTCTTCTGTTGTTTCACTTATC10.9523809523809524No Hit
AACCTCTTCAATTTCCCGAATAAGTGAAAACGTTTGCGTTTTACCATCCAT10.9523809523809524No Hit
TTGTAGAACAGGATTTAATTCTGCAAGCGGTTCGGTACAAACAACTGTCTC10.9523809523809524No Hit
GCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT10.9523809523809524No Hit
GGTGTACTAAGTGGAAAAGGATGTGGGATCGCGAAGACAGCCAGGAGGTTG10.9523809523809524No Hit
ACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAATATCTTTTT10.9523809523809524No Hit
CGATACCACGAAGTAAACAACTCTAGCATACCACCTAAAACAATTGTCATG10.9523809523809524No Hit
CCAATATCCATGTCGATGTTGTTGTATTGAACTTAAAGAGCTGTCTCTTAT10.9523809523809524No Hit
ATCTTTCCACTTGTGATGGCGACAAATAGTTAACCAAATCACTCACTTCTC10.9523809523809524No Hit
ACTTCAACAACCTCTGAAAATCTTGAGAGAAATTTCCCCCAAGAAAACATG10.9523809523809524No Hit
GACCTGTGTGCCCACTGTGGTCAACACTCAGATCAAGGACTGTCTCTTATA10.9523809523809524No Hit
CTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTGTCTCTTATAC10.9523809523809524No Hit
GCGTAATGCGTCCCTCCATCACTCCACATGATCGGTGCAGGAACTGACTCT10.9523809523809524No Hit
GTTCCTTTCTTATTGTATATCCGACACAAAACCCAATCATCAAGTCTTAAG10.9523809523809524No Hit
CAGATGTTCAGATTCGTTTCTATATGCAAAGTGATGATATTCTAGCTATTC10.9523809523809524No Hit
GCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGA10.9523809523809524No Hit
CTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTC10.9523809523809524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT10.9523809523809524No Hit
AACTTCATAAAACAAAAGAGCAAACATCTGAGATACCTGTCTCTTATACAC10.9523809523809524No Hit
ATGTCATTCTCGAATCTCAACAACCCAGGAATCATCAACTTAGCACCTGTC10.9523809523809524No Hit
ATTCCAATTTGATAGCTGCATCAAGCATATCCTGTCTCTTATACACATCTC10.9523809523809524No Hit
AGTCTGGAGAAGCGTCCTCAGCGACGGACCGGGCATAAGTTCCCTGGAAAG10.9523809523809524No Hit
CTTTATCGCTGATAATATTCCTTGTCCTAAAGTTCAAAGAGCATTGAGGTG10.9523809523809524No Hit
CCCAATGCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTC10.9523809523809524No Hit
GAGTACATAAATGCTTCAGGGGAAAAGACTCTGATATTACCCTGTCTCTTA10.9523809523809524No Hit
ATCTTGGATTTTCTGGGTAATGATACTCCACATTCAACTTTCCTTCATCAG10.9523809523809524No Hit
ATTCGTTACTCATGCCTGCATTCTCACTCGCACACAGTCCACGCCTCCTTA10.9523809523809524No Hit
ACGCAGAGTACGGGAAGCAGTGGTACCAAAAAAAAAAAAAAAAAAAAAAAA10.9523809523809524No Hit
GTCTGGGAAGGCGTACCGGAGTGGGTGAGAGTCCTGTAACTGTAAGCGTGG10.9523809523809524No Hit
ATGTGAATGTGGGTGGTTTTCTAAATAGATAGTTGGATTAGATACTGTCTC10.9523809523809524No Hit
ACGAAGTCCTTGGCTTTATCTAGCAAGCTCGCCATAATCTGTCTCTTATAC10.9523809523809524No Hit
CTTTTCTTTCAAACAACAAATCGAACTTATTCTCTATTGCAACTGTCTCTT10.9523809523809524No Hit
CAGTAATGGTCATTAGGATCTGTATTTCCATCATCAGTATTCCCTGATCCA10.9523809523809524No Hit
GTTGCAAACCGGATGGTACAAAACTGGGTTTTGGAAGATATGGCATTAAAA10.9523809523809524No Hit
ATCTGAATGGCCTGCGGTTGATATACCACTTGATCATCATGTTTTCAAAGT10.9523809523809524No Hit
GAGATATCAGAAGTGAGAATGCTGACATGAGTAACGATAAATCCTGTCTCT10.9523809523809524No Hit
CTCTGGCGCCCCTTTCCAGGGAACTTAGGCCCGGTCCGTCGCTGAGGACGC10.9523809523809524No Hit
GCTATTGACCATGGAGGGTCAACAGGCATGAAAAAAACCATCTGGCGGGGC10.9523809523809524No Hit
AGGTAGAGCACTCAATGGGCTAGGGTGGCCCCATTTCGCCTTACCAACCCC10.9523809523809524No Hit
GTACATGGGGAGTTCAGAATAAGATCGACTTTCTCTCATTATCTTATTGTC10.9523809523809524No Hit
AATCTGTACTGAGTTTTTTCAACAACTCTGGCGGAAATCTGTCTCTTATAC10.9523809523809524No Hit
TGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAA10.9523809523809524No Hit
ACATTGATGGGAATATGGTTCCACCTGGTGCTCTTATCAAATTTGTTCAGA10.9523809523809524No Hit
ACACAAGTATCCAGTGTATACAAAAAACATAAAGAGGCACCAAACCTGTCT10.9523809523809524No Hit
CACTCTAACGGCGTACCTTTTGCATGATGGGTCAGCGAGGAAATGGGAAGA10.9523809523809524No Hit
GCGGAGCAGAATATGATGGTGTCAGCCCCGGCGATGTTGTCTAGGTCAAAG10.9523809523809524No Hit
GATAATTAATTGCTGCTCATAAGTCATAACTGTTAAGAACAGCTAGTTACT10.9523809523809524No Hit
GCCTCTAATCATTGGCTTTACCCGATAGAACTCGCGTCCGAGCTCCAGCTA10.9523809523809524No Hit
ACAATGTTATGGCAGTAGGATTTCAATGTGATGCAAAATGGATGTATTCAG10.9523809523809524No Hit
ACTCGAAGCATCATATAATCTCTTAAAAGTACACAAGGACATAAGAACCTG10.9523809523809524No Hit
ACTTTTTAGTTTGTTGTGTATCATACAAATGTTTAATCCCTGTCTCTTATA10.9523809523809524No Hit
GTTTAGATAGTTGACCGTCTCATCTTCGACACAAACATCCACAGATTCTCC10.9523809523809524No Hit
CTGCATCCCACTTCGAAACAGCTGTAGAAAAATATGTTTTCGTTTTGCCTG10.9523809523809524No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.9523809523809524No Hit
GAGAATGCTGACATGAGTAACGATAAATCCTGTGAAAAACACGATCGCCTG10.9523809523809524No Hit
CCCAAAGGCTGTGCTGTACAATATTTTACTCTTTGAACTTTCAGGACTTAC10.9523809523809524No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC10.9523809523809524RNA PCR Primer, Index 27 (95% over 21bp)
GGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCACACTCGAACC10.9523809523809524No Hit
ATTCCAGGGTTTGCAGTATCTGCTGAAAGAATTTTGATGTTTTTTGATGCA10.9523809523809524No Hit
CTGTAGGACAAGCCACCGGACGGTGGTCCGATGGTTCTATCGTTCCTGATT10.9523809523809524No Hit
CTTTCGCCTTTTGAAGCGCCAGTAGCGTAGGGCGACCGGGCCAGGCCGAGT10.9523809523809524No Hit
ACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGA10.9523809523809524No Hit
CAGTTGACGTAAGAACTGATGTTCTCATCCAAAAGACCATCCGAGAAGAAT10.9523809523809524No Hit
GAGTACGGGTCAAGCATTCACAACCGTATTTGAAACAGCCTTGTCCAAATT10.9523809523809524No Hit
AAACAGATTCAATAACGTAACCAAATAACATTGGAGGCTTTTTAAAAGAAA10.9523809523809524No Hit
GTATAATGAAGAACATGCGTGGTTCCAATGAAGATCATCTTGGTGATCATT10.9523809523809524No Hit
GCTTTGGACAAGATTAGATTCGAGTCCTTGACGGACAAGAGCAAGCTCGAT10.9523809523809524No Hit
GTTCGCAAGCATTAGAGAAAACAAGAATCTGACAGTGCCTGTCTCTTATAC10.9523809523809524No Hit
CTTATGTGTCAAGGCTTGTAGTAAGACTAGTGGTGTTGAGAGCTGTCTCTT10.9523809523809524No Hit
ATTCTACCTACTCCGATTCAATCGATCTTATCGAGACCTGTCTCTTATACA10.9523809523809524No Hit
TTAGAATACAACTTTAAATGCCCATTCTCTTGAACCCTGTCTCTTATACAC10.9523809523809524No Hit
TACTTGAAGAGCACCATGGGACCACCACAAAGAATCTTTTGAGCTCTCTCT10.9523809523809524No Hit
ACTGAAATCAGCCAAACTCCGAATGCTGGCAAGTGTAGCGTGGCAGTGAGA10.9523809523809524No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10.9523809523809524No Hit
CTCGGGAGCTTTCCCCGGGCGTCGAACAGTCAGCTCAGAACTGGTACGGAC10.9523809523809524No Hit
CTCTACTTGTTCACCTGTGTCGGTTTGGGGTACGGTCAGTTCACCGGGAGG10.9523809523809524No Hit
GTCCCTAAGCAATCACTTAGTGGAAAAGGAAGTGATCGAGCGATGACAACC10.9523809523809524No Hit
GGTAATATTAACGTCTAAAACACATAAGTGGTTGTTACTCCTGTCTCTTAT10.9523809523809524No Hit
GTTCGAGAAATACTAAGCAGACATCAACTCACTGAGTTCTGTCTCTTATAC10.9523809523809524No Hit
CGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCG10.9523809523809524No Hit
AGCATTCATCATCTTGCAGTTATTCTCTATCCCTTTAGTAATGGTCCAACA10.9523809523809524No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.9523809523809524No Hit
CTATTTGACAATCACTCACTAGAGACTCCCACATTGATCCCTGTCTCTTAT10.9523809523809524No Hit
TGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAA10.9523809523809524No Hit
GTATCTCTCTTAGTCTCTTGGTGAAAGATTGGATTCTTTTTTTTTCGAGTG10.9523809523809524No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers